RESUMEN
The gene family GAGE is characterized to be expressed in testis and a portion of tumors, which is considered to be a candidate for diagnostic marker and immunotherapy target. The present study revealed that GAGE gene is unique to primate lineage. At least 15 duplicates with low divergence were found to be clustered in human X chromosome, while chimpanzee and macaque has 3 and 4 in a similar manner. The phylogenetic tree for the duplicates was constructed and the age of the duplication events was estimated, which was ranged from 4 million years ago to present. The Ka/Ks value of the duplicates was significantly greater than 1, indicating that GAGE family is under positive selection. Based on this study, GAGE may contribute to the characteristics of primate species, and the role of GAGE gene in evolution and in the genesis of gametid and tumor deserves further investigation.
Asunto(s)
Antígenos de Neoplasias/genética , Evolución Molecular , Familia de Multigenes/genética , Secuencia de Aminoácidos , Animales , Antígenos de Neoplasias/química , Secuencia de Bases , Biología Computacional , Duplicación de Gen , Humanos , Macaca mulatta/genética , Datos de Secuencia Molecular , Neoplasias/genética , Pan troglodytes/genética , Filogenia , Selección Genética , Cromosoma X/genéticaRESUMEN
OBJECTIVE: To investigate the complex differences between high metastatic and low metastatic cells of the Adenoid cystic Carcinoma. METHODS: Gene expression patterns were examined in high metastatic cell ACC-M strain and low metastatic ACC-2 strain with the method SSH (suppression subtractive hybridization). RESULTS: although extensive similarity was noted between the expression profiles, twelve genes were highly expressed of, in low metastatic cell ACC-2 tester, compared with driver, high metastatic cell ACC-M. These genes were cysteine-rich angiogenic-inducer protein (cyr61), chromosome7 clone RP11-52501, G protein, was family member Iferritin heavy polypeptide I, jumping translocation breakpoint, eukaryotic translation elongation, folate receptor, ribosomal proteins L7a, S21, P0 and other two novel genes-ACC metastasis-associated RNH and ACC metastasis-associated suspected protein. GenBank accession number were AF522024 and AF522025 respectively. CONCLUSIONS: the result suggests that the obtainment of the ability of metastasis is related to the low expression or mutation of these genes. These data provide insight into the extent of expression differences underlying metastasis-related genes that may prove useful as diagnostic or prognostic markers.
Asunto(s)
Carcinoma Adenoide Quístico/genética , Carcinoma Adenoide Quístico/secundario , Secuencia de Bases , Northern Blotting , Línea Celular Tumoral , Perfilación de la Expresión Génica , Humanos , Datos de Secuencia Molecular , Reacción en Cadena de la Polimerasa de Transcriptasa InversaRESUMEN
Metastasis and invasion, the important characteristics of malignant tumors, are closely associated with a series of changes in the expression of genes and proteins. In this study, we compare mRNA and protein expression in high and low metastasis adenoid cystic carcinoma cell lines by mRNA suppression subtractive hybridization and two-dimensional electrophoresis combined with peptide mass fingerprint analysis. 34 differentially expressed genes were obtained using suppression subtractive hybridization experiments including 6 highly expressed gene sequences in the high metastasis cell line, and 28 in the low metastasis cell line. RNA dot blot hybridization further confirmed the results after excluding false positives. For protein analysis, ten significantly different protein spots were detected using two-dimensional gel electrophoresis technique combined with matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI- TOF-MS). The results then compare with the SWISS PROT database. These results suggest that high tumor metastasis of adenoid cystic carcinoma is associated with multiple genes whose function include angiogenesis, protein synthesis, signal transduction, modulation of cell cycle, molecular chaperones, and immune co-stimulating molecule. Moreover, the results of the phenotypic function-related expression mapping analysis at the mRNA and protein level revealed obvious complementarities, providing important clues for further study of the molecular mechanism of metastasis, metastasis control and possible targets for cancer gene therapy.