RESUMEN
Butyl benzyl phthalate (BBP), a common phthalate plasticizer, is frequently detected in aquatic environments. However, there has been relatively little research on its effects on gill-related responses. This study exposed adult zebrafish to BBP concentrations ranging from 5 to 500 µg/L for 28 days, specifically investigating its toxicity in the gills. Assessment of oxidative stress biomarkers and gene expression related to apoptosis and mitochondria was conducted. Results demonstrated that exposure to 500 µg/L of BBP disrupted the antioxidant defense system, leading to lipid peroxidation and DNA damage. Moreover, the expression level of the caspase-3 gene exhibited an approximate two-fold increase, whereas the expression of 18rs-rrn decreased by 50 % on day 28. Gene Ontology enrichment analysis indicated suppressed expression of antioxidant and metabolic process terms, alongside inhibition of metabolism, immune, and signal transduction-related pathways. This study offers novel insights into the toxic effects and mechanisms of BBP on fish, providing valuable data for assessing environmental risks linked to BBP contamination and advocating for its management in aquatic ecosystems.