RESUMEN
Objective: To evaluate the role of exosomal long non-coding RNA (lncRNA) colon cancer associated transcript-2(CCAT2) in angiogenesis in nasopharyngeal carcinoma(NPC). Methods: Human umbilical vein endothelial cells (HUVECs) were divided into CNE2 supernatant coculture group and NP69 supernatant coculture group. The proliferation ability of HUVEC in each group was examined by CCK8. The lncRNA CCAT2 level in HUVEC was detected using real-time fluorescent quantitative PCR (qRT-PCR). HUVECs were divided into coculture group of serum in patients with NPC and coculture group of serum in healthy donors. The proliferation ability of HUVEC in each group was examined by CCK8. The lncRNA CCAT2 level in each group was detected using qRT-PCR. Exosomes from CNE2,NP69 supernatant were obtained by ultracentrifugation. HUVECs were divided into coculture group of CNE2's supernatant exosomes and coculture group of NP69's supernatant exosomes. Biological experiments like CCK8 were conducted to compare the angiogenesis ability of HUVEC in each group. shRNA transfection was used in HUVEC to suppress the expression of lncRNA CCAT2. Biological experiments like CCK8were conducted to compare the HUVEC's function with different expression of lncRNA CCAT2. CNE2 was transfected with shRNA, the total supernatant exosomal RNA was extracted, and the difference of exosomal lncRNA CCAT2 in CNE2 supernatant with low expression and normal expression of lncRNA CCAT2 was detected by qRT-PCR. The above mentioned two groups of exosomes were cocultured with HUVEC respectively. The difference of lncRNA CCAT2 expression in each group's HUVEC was detected by qRT-PCR and the function of HUVEC was examined with migration assay. GraphPad Prism 8 software was used for graphing and statistical analysis. Results: Compared with those in the NP69 supernatant coculture group, HUVECs in the CNE2 supernatant coculture group showed a higher proliferation ability and higher expression of lncRNA CCAT2(1 vs. 1.40±0.01, t=42.23, P=0.000). Compared with those in serum of healthy donors, HUVECs in serum of patients with NPC showed a higher proliferation ability and the expression of lncRNA CCAT2 increased (1 vs. 1.25±0.03, t=14.43,P=0.001). Compared with those in the coculture group of NP69 cells' supernatant exosomes, HUVEC in the coculture group of CNE2 cells' supernatant exosomes showed a higher proliferation ability and there were more cells that had passed the well during the migration experiment (53.12±2.13 vs. 154.74±4.17, t=37.73, P=0.000) and more knots formed in the tube-formation experiment(10.72±1.02 vs. 53.65±3.21, t=22.63, P=0.000). Compared with those in the sh-NC group, HUVEC in the sh-CCAT2 group showed a lower proliferation ability, and there were less cells that had passed the cell during the migration experiment(401.34±22.15 vs.138.25±6.85, t=23.19, P=0.000) and less MVC in the experiment of subcutaneous stroma embolization in nude mice(41.00±0.32 vs. 27.15±0.23, t=61.53, P=0.000). Compared with the supernatant exosomes in the sh-NC exosome group, the expression of lncRNA CCAT2 was lower in the supernatant exosomes of sh-CCAT2 group (1 vs. 0.65±0.02, t=30.31, P=0.000). Compared with those of the exosomes of sh-NC exosome group, the expression of lncRNA CCAT2 was lower in the HUVEC cocultured with exosomes of the sh-CCAT2 exosome group (1 vs. 0.73±0.01, t=46.77, P=0.000) and the exosomes of sh-CCAT2 exosome group showed a lower migration ability (389.73±26.34 vs. 190.54±8.36, t=12.47, P=0.001) after cocultured with HUVEC. Conclusion: Nasopharyngeal carcinoma-derived exosomal lncRNA CCAT2 could promote angiogenesis.
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Neoplasias del Colon , Exosomas , Neoplasias Nasofaríngeas , ARN Largo no Codificante , Animales , Línea Celular Tumoral , Proliferación Celular , Células Endoteliales , Exosomas/genética , Humanos , Ratones , Ratones Desnudos , Carcinoma Nasofaríngeo , ARN Largo no Codificante/genéticaRESUMEN
The aim of this study was to investigate the mechanism of hepatocyte apoptosis and regeneration after partial hepatectomy in obstructive jaundice (OJ) rats under different drainage methods of bile acid intervention. Forty male Sprague Dawley rats were randomly divided into five groups. An OJ rat model was established by the following protocols. Seven days after obstruction, an SD rats model with 70% partial hepatectomy was established by different drainage methods of OJ. Blood and liver tissue samples were collected from rats 72 h after surgery; 72 h after partial hepatectomy (PH), the liver regeneration rate, the expression of proliferating cell nuclear antigen (PCNA) and the level of mitotic index (MI) in the internal biliary drainage (IBD) group were higher than those in external biliary drainage (EBD) group (P less than 0.05). Those in the EBD group were higher compared to the OJ group (P less than 0.05). There was no significant difference among the IBD group, EBD+CA group and (SO) sham operation group (P>0.05). Bax expressions had the same trend as AI in the five groups. The expression of Bcl-2 was increased in the IBD group and EBD+CA group, which was statistically higher compared to the SO group (P less than 0.05). In conclusion, both internal and external drainage can relieve biliary obstruction. The difference in liver regeneration caused by external drainage and internal drainage may be attributed to the destruction of bile acid enterohepatic circulation, which increases hepatocyte apoptosis and affects liver regeneration.
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Apoptosis , Ácidos y Sales Biliares/sangre , Drenaje/métodos , Hepatocitos/patología , Ictericia Obstructiva/patología , Regeneración Hepática , Animales , Hepatectomía , Hígado/patología , Masculino , Distribución Aleatoria , Ratas , Ratas Sprague-DawleyRESUMEN
BACKGROUND: Eosinophilic gastroenteritis (EG) is uncommon disease, and the pathogenesis of this disease have yet to be fully clarified. AIM: This study was to describe the clinical manifestations, endoscopic features and treatment outcomes of a cohort of patients with EG. METHOD: This retrospective study was included 28 consecutive patients who were diagnosed EG between January 2011 and December 2015 in Taizhou Hospital. The patients' clinical manifestations, endoscopic features and treatment outcomes were reviewed from a prospectively maintained database. RESULTS: Twenty-eight patients with EG were enrolled in the study (median age 54 years). The main symptoms were abdominal pain (78.6%), abdominal distension (50.0%), nausea and vomiting (28.6%) and diarrhea (25.0%). Laboratory examinations showed the elevation of blood eosinophil count (85.7%), serum IgE (71.4%). Endoscopic findings included small patchy mucosal erythema or erosions (75.0%), mucosal fold thickening (17.9%), submucosal nodules (21.4%), small gastroduodenal ulcers (14.3%). Twenty patients were treated and responded to prednisolone but five patients (25.0%) relapsed during the follow-up. The other 8 patients were treated with loratadine, proton pump inhibitors and dietary modification, 5 patients had clinical resolution during the follow-up. The other 3 patients did not achieve clinical remission, and then were given prednisone treatment. CONCLUSION: For some patients with gastrointestinal symptoms and peripheral eosinophilia, a high suspicion of EG is necessary and multiple endoscopic examinations might be helpful in diagnosis of EG. Most patients with EG could achieve remission after with the treatment of steroid or dietary elimination therapy.
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Enteritis/diagnóstico , Enteritis/tratamiento farmacológico , Eosinofilia/diagnóstico , Eosinofilia/tratamiento farmacológico , Gastritis/diagnóstico , Gastritis/tratamiento farmacológico , Dolor Abdominal/etiología , Adulto , Anciano , Femenino , Glucocorticoides/uso terapéutico , Humanos , Masculino , Persona de Mediana Edad , Prednisolona/uso terapéutico , Inhibidores de la Bomba de Protones/uso terapéutico , Estudios Retrospectivos , Resultado del Tratamiento , Vómitos/etiologíaRESUMEN
BACKGROUND: In the concept of cloud-computing-based systems, various authorized users have secure access to patient records from a number of care delivery organizations from any location. This creates a growing need for remote visualization, advanced image processing, state-of-the-art image analysis, and computer aided diagnosis. OBJECTIVES: This paper proposes a system of algorithms for automatic detection of anatomical landmarks in 3D volumes in the cloud computing environment. The system addresses the inherent problem of limited bandwidth between a (thin) client, data center, and data analysis server. METHODS: The problem of limited bandwidth is solved by a hierarchical sequential detection algorithm that obtains data by progressively transmitting only image regions required for processing. The client sends a request to detect a set of landmarks for region visualization or further analysis. The algorithm running on the data analysis server obtains a coarse level image from the data center and generates landmark location candidates. The candidates are then used to obtain image neighborhood regions at a finer resolution level for further detection. This way, the landmark locations are hierarchically and sequentially detected and refined. RESULTS: Only image regions surrounding landmark location candidates need to be trans- mitted during detection. Furthermore, the image regions are lossy compressed with JPEG 2000. Together, these properties amount to at least 30 times bandwidth reduction while achieving similar accuracy when compared to an algorithm using the original data. CONCLUSIONS: The hierarchical sequential algorithm with progressive data transmission considerably reduces bandwidth requirements in cloud-based detection systems.
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Anatomía/instrumentación , Inteligencia Artificial , Interpretación de Imagen Asistida por Computador/instrumentación , Patología/instrumentación , Acceso a la Información , Algoritmos , Humanos , Interpretación de Imagen Asistida por Computador/métodos , Imagen por Resonancia Magnética/instrumentación , Informática Médica/instrumentación , Tomografía Computarizada por Rayos X/instrumentación , Tomografía Computarizada por Rayos X/métodosRESUMEN
It is generally believed that glycoprotein hormone exists in granules of the gonadotrophin (GTH) cells, and recent studies on the formation and secretion of glycoprotein hormones also concern much on the changes in the pituitary granules. In our previous studies, it was found that the secretion of luteinizing hormone (LH) was closely related to the morphological changes in pituitary vacuoles of rats, and the morphology of GTH cells was also closely related to the changes in vacuolar morphology. Therefore, it appears that there exists an inseparable relationship between the storage and secretion of LH and pituitary vacuoles. In order to examine whether the pituitary vacuoles contain LH in rats, and to further explore if LH is stored and released by the pituitary vacuoles, the vacuoles were separated and purified from pituitary, cortex and liver tissues by density gradient centrifugation. The vacuolar protein components, LH and glycoproteins were determined by the methods of SDS-PAGE, Western immunoblot and Con A/HRP, respectively. The results showed that (1) abundant protein components with various molecular masses were present in the vacuoles of the pituitary, cortex and liver in male, female and ovariectomized rats, and some of the protein components were similar in the molecular mass; (2) both pituitary tissues and vacuoles contained LH, and their LH levels had no significant difference by quantitative analysis of extracted protein samples; and (3) glycoproteins of various molecular masses were found in the vacuoles of the pituitary, cortex and liver, but the same position of stained glycoprotein band and LH molecular mass was only observed in the pituitary vacuoles. From the results given above, it can be concluded that LH specifically exists in the pituitary vacuoles, although there are some similar protein components in various cellular vacuoles. The possibility was further demonstrated at the molecular level that the pituitary vacuoles may have the function of storing and releasing LH due to the pituitary vacuoles containing LH with assembled glycone.
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Hormona Luteinizante/análisis , Hipófisis/metabolismo , Vacuolas/metabolismo , Animales , Femenino , Glicoproteínas/análisis , Gonadotropinas/análisis , Masculino , Hipófisis/citología , Ratas , Ratas Sprague-DawleyRESUMEN
OBJECTIVE: To evaluate the MRI in the differential diagnosis of acute non-traumatic vertebral collapse. METHODS: Sixty-eight cases were retrospected. All cases were studied with sagittal T1-weighted MR images, T2-weighted MR images, gadolinium-enhanced T1-weighted MR images, and axial T1-weighted MR images. Twelve of 68 were performed extra coronal T1-weighted MR images and 22 with additional axial gadolinium-enhanced T1-weighted images. RESULTS: The causes of vertebral collapse were: (1) osteoporosis (n = 31), their images showed retropulsion of bony fragments of collapsed vertebral body which kept the normal signal into the spinal canal as the distinctive sign; (2) metastatic tumor (n = 30), their images showed destructions of pedicles of vertebral and epidural mass as the distinctive sign, and hyperintense or mixed hyperintense signal on T2WI and enhanced T1WI of compressed vertebrae; and (3) multiple myeloma (n = 7), their images showed long T1WI and long T2WI, without destruction of pedicle and epidural mass in compressed vertebrae. CONCLUSION: MRI is useful to the differentiations of the non-traumatic vertebral collapse in aged.
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Imagen por Resonancia Magnética , Osteoporosis/complicaciones , Fracturas de la Columna Vertebral/diagnóstico , Anciano , Anciano de 80 o más Años , Femenino , Humanos , Vértebras Lumbares , Masculino , Persona de Mediana Edad , Mieloma Múltiple/complicaciones , Estudios Retrospectivos , Fracturas de la Columna Vertebral/etiología , Vértebras TorácicasRESUMEN
The relationship between the peak of LH release induced by exogenous LHRH and morphological change of LH cells in male rats was investigated by ABC Affinity histochemistry and qualitatively characterized by Medical Image Processing System-I. The serum LH concentration was determined by RIA. During the period of basal secretion of LH (3.76 +/- 0.39 ng/ml), the LH cells might be assigned at a storage state. Most of such cells (56.7%) are round and large, and their cross sectional areas are larger then 340 micron 2, usually containing large vacuoles, while the smaller cells of X-sectional area less then 190 micron 2 counted only 2% of the total population. Thirty minutes after injection of LHRH the serum LH level increased significantly (6.46 +/- 1.6 ng/ml, P < 0.01), there appeared a lot of small angular cells, the average cross sectional areas of LH cells decreased significantly (P < 0.0001). When LHRH was injected at sixty minutes, the LH level reached a peak (18.78 +/- 0.79 ng/ml), the number of the large cells decreased to only 4%, while the small cells increased to 66%, vacuoles were almost disapperanced and a large part of cells (52.6%) became irregular. Thereafter, the LH serum gradually subsided to its original basal level with attendant resumption of the morphology of the LH cells at storage state. It appears that the morphological changes, such as size, shape, vacuoles and so on, of the LH cells may serve as an important index for showing the effect of LHRH on LH secretion.