RESUMEN

Ovarian cancer (OC) is a highly lethal disease among all gynecologic malignant tumor types. Accumulating studies have indicated that certain long non-coding RNAs (lncRNAs) serve important roles in the development and progression of OC. In a previous study by our group, lncRNA BC041954 was identified as one of the most upregulated lncRNAs in OC. In the present study, the clinical significance of lncRNA BC041954 in OC was evaluated. The expression of BC041954 was detected in OC and non-tumor tissue (NT) samples using reverse transcription-quantitative PCR. Furthermore, the association between BC041954 and clinicopathological variables was analyzed using the Chi-square test. Survival was determined using Kaplan-Meier analysis. The prognostic significance of BC041954 was evaluated using univariate and multivariate logistic regression analyses. MicroRNA (miRNA)-lncRNA and miRNA-mRNA pairs were used to construct the lncRNA-miRNA-mRNA competing endogenous RNA network with an in-house Perl script. BC041954 expression was increased in 103 OC tissues as compared with that in NT tissues. Upregulated BC041954 expression was significantly associated with the International Federation of Gynecology and Obstetrics stage and distant metastasis. Kaplan-Meier analysis demonstrated that patients with high BC041954 expression had lower overall survival (OS). In the multivariate logistic regression analysis, BC041954 was also identified as an independent poor prognostic factor for OS in patients with OC. The results suggested that overexpression of the lncRNA BC041954 is a poor prognostic indicator in patients with OC.

RESUMEN

BACKGROUND: The adverse effects of TI exposure on pregnant women are still unclear, especially regarding the risk of gestational diabetes mellitus (GDM) Objective: We explored the association between maternal urinary Tl burden and the risk of GDM. METHODS: A subsample of 1789 pregnant women were enrolled who provided spot urine samples before the diagnostic 75-g oral glucose tolerance test. Urinary Tl concentration was measured using inductively coupled plasma mass spectrometry. Logistic regression and covariance analysis were carried out to estimate the association between Tl exposure and GDM risk. RESULTS: The median of urinary Tl concentration was 0.382 µg/L or 0.525 µg/g creatinine (CC-Tl). There were 437 (24.4%) participants who were diagnosed with GDM, and the urinary CC-Tl concentrations of pregnant women with GDM were higher than that of pregnant women without GDM [0.548 (0.402, 0.788) vs 0.518 (0.356, 0.724), p = 0.014]. After adjusting for the relevant covariates, an association between urinary Tl concentrations and GDM was found. In comparison to the pregnant women in the lowest quartile of urinary CC-Tl concentration, the pregnant women in the highest quartile had a higher risk of GDM [OR (95% CI) = 1.44 (1.03, 2.02), p-trend = 0.055]. If limited to the pregnant women without family history of diabetes, the results were still robust [OR (95% CI) = 1.59 (1.11, 2.30), p-trend = 0.012]. CONCLUSION: Urinary CC-Tl concentration was associated with GDM among Chinese pregnant women. Our findings provide evidence that moderately high Tl exposure may be a novel risk factor for pregnant women health.

Asunto(s)

Diabetes Gestacional , China , Estudios de Cohortes , Diabetes Gestacional/inducido químicamente , Diabetes Gestacional/epidemiología , Femenino , Humanos , Exposición Materna/efectos adversos , Embarazo , Factores de Riesgo , Talio

RESUMEN

STUDY OBJECTIVE: Cesarean scar pregnancy (CSP) is a rare type of ectopic pregnancy, and a significant concern in the management of this condition is the control and prevention of bleeding. We aimed to determine the efficacy and value of an indwelling, intrauterine Foley balloon catheter in controlling and preventing intraoperative and postoperative bleeding in patients with CSP. DESIGN: Retrospective case series. SETTING: University-affiliated hospital. PATIENTS: Between January 1, 2015 and May 31, 2017, 70 patients presented with CSP. INTERVENTIONS: All patients underwent uterine curettage under hysteroscopic guidance and ultrasound monitoring. Patients were then assigned to 2 groups: the study group, with an indwelling Foley balloon catheter placed in the uterine cavity during surgery and retained for 24 to 48 hours, and the control group, without catheter placement. Data were collected to compare the 2 groups in terms of intraoperative and postoperative complications, surgical time, and status of menstruation resumption. MEASUREMENTS AND MAIN RESULTS: The average daily volume of postoperative blood loss during the first 3 postoperative days in the study group was 23.1 mL compared with 31.5 mL observed in the control group (p = .041). Moreover, the study group had significantly shorter average duration of bleeding (p = .027) and fewer menstruation abnormalities than the control group. Uterine ultrasonography performed after resumption of menstruation showed that none of the enrolled patients had any intrauterine abnormalities. CONCLUSIONS: The use of an indwelling, intrauterine Foley balloon catheter has positive results in the management of CSP.

Asunto(s)

Oclusión con Balón , Cesárea/efectos adversos , Cicatriz/cirugía , Hemorragia Posoperatoria/terapia , Embarazo Ectópico/terapia , Cateterismo Urinario , Adulto , Oclusión con Balón/efectos adversos , Oclusión con Balón/instrumentación , Oclusión con Balón/métodos , Estudios de Casos y Controles , Catéteres de Permanencia/efectos adversos , Cicatriz/complicaciones , Femenino , Humanos , Persona de Mediana Edad , Tempo Operativo , Complicaciones Posoperatorias/etiología , Complicaciones Posoperatorias/terapia , Hemorragia Posoperatoria/etiología , Embarazo , Embarazo Ectópico/etiología , Estudios Retrospectivos , Resultado del Tratamiento , Cateterismo Urinario/efectos adversos , Cateterismo Urinario/instrumentación , Cateterismo Urinario/métodos , Útero/cirugía

RESUMEN

Shift work and jet lag, characterized by circadian misalignment, can disrupt several physiological activities, but whether they affect the rhythm of glucose uptake and insulin sensitivity remain unclear. In the present study, female C57BL/6J mice were maintained for four weeks under the condition of 8-hour phase advance and delay every 3-4 days to mimic shift work. Intraperitoneal glucose tolerance test (IPGTT) and intraperitoneal insulin tolerance test (IPITT) were performed repeatedly at Zeitgeber time (ZT) 0, ZT6, ZT12, and ZT18. Glucose-stimulated insulin secretion (GSIS) test was performed at ZT6. We found that the average level of daily glucose tolerance did not decrease but the phase of glucose tolerance advanced by 2.27 hours and the amplitude attenuated by 20.4% in shift work mice. At ZT6, IPITT showed blood glucose at 30 min after insulin injection decreased faster in shift work mice (-3.50±0.74mmol/L, -61.58±7.89%) than that in control mice (-2.11±1.10mmol/L, -33.72±17.24%), but IPGTT and GSIS test showed no significant difference between the two groups. Food intake monitor showed that the feeding time of shift work mice continued to advance. Restricting feed to a fixed 12-hour period alleviated the increase of insulin sensitivity induced by shift-work. We also observed that an increase of blood glucose and liver glycogen at ZT0, as well as a phase advance of liver clock genes and some glucose metabolism-related genes such as forkhead box O1 (Foxo1) and peroxisome proliferator activated receptor alpha (Pparα) in shift work mice. Our results showed that light change-simulated shift work altered insulin sensitivity during the light phase and shifted glucose tolerance rhythms in female mice, suggesting a causal association between long-term shift work and type 2 diabetes.

Asunto(s)

Glucemia/metabolismo , Ritmo Circadiano/fisiología , Conducta Alimentaria/fisiología , Resistencia a la Insulina/fisiología , Insulina/sangre , Horario de Trabajo por Turnos/psicología , Animales , Femenino , Prueba de Tolerancia a la Glucosa/métodos , Ratones , Ratones Endogámicos C57BL

RESUMEN

The dysregulation of long non­coding RNAs (lncRNAs) is associated with cancer development. The present study profiled differentially expressed lncRNAs in ovarian cancer (OC) versus normal ovarian tissues (NT) and investigated their potential functions in gene expression. OC tissues from 30 patients and NT specimens from 20 non­tumor patients were collected, and 5 cases of tumor and NT were subjected to lncRNA and mRNA microarray analysis. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway analyses were performed. Reverse transcription­quantitative polymerase chain reaction (RT­qPCR) was used to verify microarray data in all 30 cases. There were 2,870 differentially expressed lncRNAs (795 upregulated and 2,075 downregulated) and 2,658 differentially expressed mRNAs (1,014 upregulated and 1,644 downregulated) in OC. A total of 4 upregulated and 4 downregulated lncRNAs were validated using RT­qPCR. The data demonstrated that, with the exception of ENST00000453838 and ENST00000505048, the lncRNAs were consistent with the microarray data. Another differentially expressed lncRNA (BC041954) was assessed using independent tissue samples, and results further supported the microarray data. Moreover, GO analysis showed that the upregulated genes were involved in the 'development of the cell anatomical structure' (GO: 0048856; P=5.46x10­6), 'embryo and system development' and 'multicellular organismal development' in biological processes. By contrast, the downregulated genes were involved in 'gene expression' (GO: 0010476; P=1.81x10­6), 'nitrogen compound metabolic process', 'kidney development' and the 'cellular nitrogen compound metabolic process'. These differentially expressed lncRNAs could be classified into four classes, namely, the enhancer lncRNA nearby coding gene, HOX cluster, long­intergenic non­coding RNAs (lincRNAs) nearby coding gene and Rinn lincRNAs. Coding­non­coding gene co­expression network analysis showed the interregulation of lncRNAs and mRNAs in OC development. In conclusion, dysregulated lncRNA and mRNA expression could promote OC development. Further study may validate a number as OC markers and provide novel insights into ovarian cancer biology.

Asunto(s)

Regulación Neoplásica de la Expresión Génica , Neoplasias Ováricas/genética , ARN Largo no Codificante/metabolismo , ARN Mensajero/metabolismo , Adulto , Anciano , Conjuntos de Datos como Asunto , Regulación hacia Abajo , Femenino , Perfilación de la Expresión Génica/métodos , Redes Reguladoras de Genes , Humanos , Persona de Mediana Edad , Análisis de Secuencia por Matrices de Oligonucleótidos , Ovario/patología , ARN Largo no Codificante/genética , ARN Mensajero/genética , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Transducción de Señal , Regulación hacia Arriba