RESUMEN
Dental pulp inflammation is a common and significant factor related to poor dental prognosis. Current treatment strategies primarily concentrate on managing the inflammatory response, with specific targets for intervention still under investigation. Triggering receptors expressed on myeloid cells (TREMs) are a group of receptor molecules extensively present on myeloid cell surfaces, crucial in the regulation of inflammatory process. Our analysis of transcriptomic sequencing data from clinical pulp samples of dataset GSE77459 and animal models revealed up-regulation of Trem1 during pulpitis. Administration of the Trem1-blocking peptide LP17 led to lower (more than 1-fold) levels of several pro-inflammatory factors and inhibition of M1 macrophage polarization both in vivo and in vitro. This study of the expression patterns and functions of Trem1 in the development of dental pulp inflammation provides novel insights into the therapeutic strategies for clinical pulpitis.
RESUMEN
Garlic (Allium sativum L.), which is a widely distributed plant, is globally used as both spice and food. This study identified five novel phenolic compounds, namely, 8-(3-methyl-(E)-1-butenyl)diosmetin, 8-(3-methyl-(E)-1-butenyl)chrysin, 6-(3-methyl-(E)-1-butenyl)chrysin, and Alliumones A and B, along with nine known compounds 6-14 from the ethanol extract of garlic. The structures of these five novel phenolic compounds were established via extensive 1D- and 2D-nuclear magnetic resonance spectroscopy experiments. The effects of the phenolic compounds isolated from garlic on the enzymatical or nonenzymatical formation of sulfur-containing compounds produced during garlic processing were examined. Compound 12 significantly reduced the thermal decomposition of alliin, whereas compound 4 exhibited the highest percentage of alliinase inhibition activity (36.6%).
Asunto(s)
Manipulación de Alimentos , Ajo/química , Fenoles/farmacología , Compuestos de Azufre/antagonistas & inhibidores , Compuestos Orgánicos Volátiles/antagonistas & inhibidores , Liasas de Carbono-Azufre/antagonistas & inhibidores , Cisteína/análogos & derivados , Cisteína/química , Calor , Espectroscopía de Resonancia Magnética , Estructura Molecular , Fenoles/análisis , Fenoles/química , Extractos Vegetales/química , Compuestos de Azufre/análisis , Compuestos Orgánicos Volátiles/químicaRESUMEN
(R)-3-(allylthio)-2-((R)-3-(allylthio)-2-aminopropanamido)propanoic acid was isolated from the bulb of garlic, together with four known amino acids. Its structure was elucidated on the basis of 2D NMR and MS techniques. To the best of our knowledge, (R)-3-(allylthio)-2-((R)-3-(allylthio)-2-aminopropanamido)propanoic acid, which showed antibacterial activity against the Staphylococcus aureus antibiotic resistant strain, was the first example of dipeptide from garlic.
Asunto(s)
Antibacterianos/aislamiento & purificación , Dipéptidos/aislamiento & purificación , Medicamentos Herbarios Chinos/aislamiento & purificación , Ajo/química , Staphylococcus aureus Resistente a Meticilina/efectos de los fármacos , Antibacterianos/química , Antibacterianos/farmacología , Dipéptidos/química , Dipéptidos/farmacología , Medicamentos Herbarios Chinos/química , Medicamentos Herbarios Chinos/farmacología , Pruebas de Sensibilidad Microbiana , Estructura Molecular , Resonancia Magnética Nuclear Biomolecular , Raíces de Plantas/química , EstereoisomerismoRESUMEN
The title mol-ecule, C(16)H(18)Se(2), features a diselenide bridge between two 4-methyl-benzyl units, in which the central C-Se-Se-C torsion angle is 88.1â (3)°, while the two Se-Se-C-C fragments assume gauche conformations, with torsion angles of -51.8â (5) and 59.1â (4)°. The dihedral angle between the benzene rings is 78.9â (2)°.
RESUMEN
The title mol-ecule, C(14)H(10)Cl(4)Se, features a selenide bridge between two dichloro-benzyl units. The dihedral angle between the two benzene rings is 107.9â (16)°. In the crystal, weak π-π face-to-face aromatic inter-actions are observed [centroid-centroid distance between two adjacent (but crystallographically different) phenyl rings = 3.885â (5)â Å], providing some packing stability. Short Clâ¯Cl contacts of 3.41â (2)â Å are observed.
RESUMEN
The title Schiff base compound, C(13)H(16)N(3)O(+)·PF(6) (-), was derived from the condensation of 2-hydroxy-benaldehyde with the ionic liquid 1-(2-amino-ethyl)-3-methyl-imidazolium hexa-fluoro-phosphate in an ethanol solution. The asymmetric unit comprises one cation and two PF(6) (-) anions. The dihedral angle between the aromatic and imidazole rings is 15.2â (2)°. An intra-molecular O-Hâ¯N hydrogen bond is found which generates an S(6) ring motif.
RESUMEN
OBJECTIVE: To observe the effects of Xuanyinning Recipe (XYNR) in inhibiting SPC-A-1 cellular infiltration and on the pathomorphological changes of peritoneum in mice inoculated with sarcoma 180 (S(180)). METHODS: On the bases of isolated culture of mouse peritoneal mesothelial cells, we adjusted and added the human lung adenocarcinoma cell line SPC-A-1 into the double-layer culture medium to observe the number of clones formed. We also took out the peritoneum from the mice administered with three different dosages of XYNR and observed its pathomorphological changes with transmission electron microscope. RESULTS: In the in vitro experiment, the number of clones of SPC-A-1 in culture medium with XYNR (50 microg/ml) decreased distinctly. In the in vivo experiment, it was observed that, in the peritoneum from the XYNR-treated mice inoculated with S(180), the mesothelial cells arranged more and more regularly with the increasing of the dosage of XYNR, while the mesothelial cells in the peritoneum of the mice in the control group necrosed and arranged loosely. CONCLUSION: XYNR can inhibit the invasion of SPC-A-1 cells. It also can improve the loose arrangement of the peritoneal mesothelial cells in mice inoculated with S(180), so as to inhibit the malignant effusion.