RESUMEN
To study the triterpenoids from the roots of Rosa laevigata. The silica gel column chromatography was used to separate the chemical constituents from the roots of Rosa laevigata Michx. HPLC was used to analyze its purity, chemical and spectroscopy methods were used to determine their structures. 12 constituents were isolated and identified as(2R, 19R)methyl 2-acetyloxy-19- hydroxyl-3-oxo-urs-12-en-28-carboxylate(1), pomonic acid (2), 18, 19-seco, 2α, 3α-dihydroxy-19-oxo-urs-11, 13(18)-dien-28-oic acid(3), swinhoeic acid (4), myrianthic acid(5), 2α, 3ß, 19α-trihydroxy-24-oxo-urs-12-en-oic acid (6), tormentic acid(7), arjunic acid (8), 1ß-hydroxyeuscaphic acid(9), quadranoside â § (10), alpinoside(11), rubuside B (12). Compounds 1-4, 6, 9, 11-12 were obtained from this plant for the first time. Compounds 2-4, 6, 11-12 were obtained from the genus Rosa for the first time.
Asunto(s)
Raíces de Plantas/química , Rosa/química , Triterpenos/aislamiento & purificación , Cromatografía Líquida de Alta Presión , Estructura Molecular , Fitoquímicos/aislamiento & purificaciónRESUMEN
OBJECTIVE: To study the extraction technology with CO2 supercritical fluid in Chinese Traditional Medicine by extracting the anthraquinones ingredients from Salvia miltiorrhiza and Polygonum caspidatum. METHOD: Extractions were made under different pressure, temperature, extraction time, entrainer, and the extracts were analyzied by TLC and HPLC. RESULT: The technology could be used in extraction and isolation. Under certain conditions, different contents of the extracts could be obtained. CONCLUSION: CO2 supercritical fluid is good at extracing low polarity ingredients, and it shows the evident individurity in extraction of Chinese herb. The technological factors in industrial usage must be thought of all over.