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Antonie Van Leeuwenhoek ; 102(1): 99-104, 2012 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-22407501

RESUMEN

Two strains of a basidiomycetous yeast were derived from an insect trypanosomatid culture isolated from the intestine of a plant bug, Collaria oleosa (Heteroptera: Miridae), collected in Costa Rica. The yeast did not form ballistoconidia but reproduced only by budding. Teliospores were not observed in individual and crossed cultures of each strain. Morphological and other taxonomic characteristics of the yeast were similar to those of the species in the polyphyletic genus Rhodotorula. However, molecular phylogeny inferred from the internal transcribed spacers and D1/D2 region of the large subunit rRNA gene showed that the strains represent a new species placed among the smut fungi in the family Ustilentylomataceae, which includes Aurantiosporium subnitens, Fulvisporium restifaciens, Ustilentyloma fluitans, and Rhodotorula hordea. Given the well distinguished phylogenetic position of this novel species within the Ustilentylomataceae, we propose Microbotryozyma collariae gen. nov., sp. nov. to accommodate the yeast isolated from C. oleosa, with strain American Type Culture Collection MYA-4666(T) (= PRA303-1S = CBS 12537) designated as the type strain.


Asunto(s)
Basidiomycota/clasificación , Basidiomycota/aislamiento & purificación , Heterópteros/microbiología , Animales , Basidiomycota/genética , Basidiomycota/fisiología , Análisis por Conglomerados , Costa Rica , ADN de Hongos/química , ADN de Hongos/genética , ADN Espaciador Ribosómico/química , ADN Espaciador Ribosómico/genética , Intestinos/microbiología , Datos de Secuencia Molecular , Filogenia , Saccharomycetales , Análisis de Secuencia de ADN
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