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1.
Braz J Med Biol Res ; 48(8): 743-50, 2015 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-26108097

RESUMEN

The radial approach is widely used in the treatment of patients with coronary artery disease. We conducted a meta-analysis of published results on the efficacy and safety of the left and right radial approaches in patients undergoing percutaneous coronary procedures. A systematic search of reference databases was conducted, and data from 14 randomized controlled trials involving 6870 participants were analyzed. The left radial approach was associated with significant reductions in fluoroscopy time [standardized mean difference (SMD)=-0.14, 95% confidence interval (CI)=-0.19 to -0.09; P<0.00001] and contrast volume (SMD=-0.07, 95%CI=-0.12 to -0.02; P=0.009). There were no significant differences in rate of procedural failure of the left and the right radial approaches [risk ratios (RR)=0.98; 95%CI=0.77-1.25; P=0.88] or procedural time (SMD=-0.05, 95%CI=0.17-0.06; P=0.38). Tortuosity of the subclavian artery (RR=0.27, 95%CI=0.14-0.50; P<0.0001) was reported more frequently with the right radial approach. A greater number of catheters were used with the left than with the right radial approach (SMD=0.25, 95%CI=0.04-0.46; P=0.02). We conclude that the left radial approach is as safe as the right radial approach, and that the left radial approach should be recommended for use in percutaneous coronary procedures, especially in percutaneous coronary angiograms.


Asunto(s)
Enfermedad de la Arteria Coronaria/cirugía , Intervención Coronaria Percutánea/métodos , Arteria Radial/cirugía , Enfermedad de la Arteria Coronaria/diagnóstico por imagen , Fluoroscopía/métodos , Humanos , Intervención Coronaria Percutánea/efectos adversos , Arteria Radial/diagnóstico por imagen , Ensayos Clínicos Controlados Aleatorios como Asunto , Arteria Subclavia/anatomía & histología , Factores de Tiempo
2.
Genet Mol Res ; 13(3): 7356-64, 2014 Sep 12.
Artículo en Inglés | MEDLINE | ID: mdl-25222234

RESUMEN

Numerous studies have evaluated the association between MTHFR C677T polymorphism and osteoporotic fracture risk in postmenopausal women. However, the results have been inconsistent. We performed a meta-analysis of the association between MTHFR C677T polymorphism and osteoporotic fracture risk in postmenopausal women. Only seven case-control studies were retrieved, with a total of 4258 patients and 3454 healthy controls. Meta-analysis results showed no significant association between MTHFR C677T polymorphism and osteoporotic fracture risk in postmenopausal women for all genetic models (for TT vs CC: OR=0.99, 95%CI=0.72-1.39; for TT vs TC: OR=1.02, 95%CI=0.87-1.20; for CC+TC vs TT: OR=0.96, 95%CI=0.71-1.28; for TT+TC vs CC: OR=0.93, 95%CI=0.84-1.03). In the subgroup analysis by ethnicity, the results also showed no significant association between MTHFR C677T polymorphism and susceptibility to osteoporotic fracture in postmenopausal women in both Caucasian and Asian populations. In conclusion, this meta-analysis suggests that MTHFR C677T polymorphism may not be associated with susceptibility to osteoporotic fracture in postmenopausal women.


Asunto(s)
Metilenotetrahidrofolato Reductasa (NADPH2)/genética , Fracturas Osteoporóticas/etiología , Polimorfismo de Nucleótido Simple , Posmenopausia , Alelos , Estudios de Casos y Controles , Femenino , Estudios de Asociación Genética , Genotipo , Humanos , Oportunidad Relativa , Sesgo de Publicación
3.
Genet Mol Res ; 13(2): 4326-35, 2014 Jun 09.
Artículo en Inglés | MEDLINE | ID: mdl-25036177

RESUMEN

Bone morphological protein7 (BMP7), a member of the transforming growth factor-ß (TGF-ß) family, was first identified because of its ability to induce ectopic chondro-osteogenesis in vivo. It also plays a crucial role in the growth, development, and physiological functioning of the reproductive system. Among intra-ovarian growth factors, many studies have shown that BMP7 plays a pivotal role in regulating the early phases of follicular growth. We detected a 5-bp insertion-deletion at 602 bp upstream from the transcription start site of the BMP7 gene promoter among 258 pigs of 3 breeds. Along with 2 homoduplex DNAs, another 4 previously unknown bands (named A, B, C, and D) were detected by non-denaturing polyacrylamide gel electrophoresis. By DNA sequencing, we found that PCR products from heterozygotes contained 2 homoduplexes and 4 heteroduplexes. Genetic polymorphism analysis revealed 3 genotypes (AA, AB, and BB) at this site; the distribution of these genotypes followed Hardy-Weinberg equilibrium. A was the dominant allele (0.715), and AA was the dominant genotype (0.500). The polymorphism information content value was calculated to be 0.325, the expected heterozygosity was 0.407, and effective number of alleles was 1.688, indicating an intermediate degree of polymorphism and good potential for selection and breeding. Highly significant differences were found between different breeds and distributions of genotypes. Based on correlation analysis, the 5-bp indel site does not significantly affect porcine reproductive traits (total number of births, number of piglets born alive, litter birth weight, and litter weight at 21 days; P>0.05), which was consistent with the results of genetic variation analysis.


Asunto(s)
Peso al Nacer , Proteína Morfogenética Ósea 7/genética , Mutación INDEL , Regiones Promotoras Genéticas , Sus scrofa/genética , Porcinos/genética , Animales , Cruzamiento , Genotipo , Polimorfismo Genético , Análisis de Secuencia de ADN , Sus scrofa/clasificación , Sus scrofa/fisiología , Porcinos/clasificación
4.
Genet Mol Res ; 13(2): 4617-26, 2014 Jun 18.
Artículo en Inglés | MEDLINE | ID: mdl-25036511

RESUMEN

This study aimed to investigate the effects of thrombin released in hematoma after intracerebral hemorrhage (ICH) on the cerebral injury of perihematomal tissues and to evaluate the protection effect of hirudin on the cerebral injury after ICH. We used the autologous uncoagulated blood injection method to prepare the ICH rat model, and all rats were randomly divided into a normal group, an ICH group, or a hirudin group. At different time points, rat heads were cut to harvest brain sections. Immunohistochemical staining, histochemical staining, and hematoxylin and eosin staining were conducted for CD34, microglia, and neutrocytes. CD34-positive microvessels were most abundant in brain tissues of the sham-operation group. At 12 h after ICH, CD34 expression reduced and reached the minimum level at 72 h (P<0.01). At 6 h after ICH, microglia expression was visible and reached a peak at 48 h (P<0.01). At 12 h after ICH, neutrocyte infiltration was visible and the number was greatest at 48 h (P<0.01). The early application of hirudin after ICH could significantly reduce microglia and neutrocyte expression and could significantly slow down the CD34 decrease trend (P<0.01). However, hirudin application in the edematization stage after ICH did not significantly increase CD34- positive microvessel abundance (P>0.05). A thrombin-mediated inflammatory reaction is involved in the cerebral injury after ICH, and the early application of hirudin has a protective effect.


Asunto(s)
Edema Encefálico/complicaciones , Edema Encefálico/metabolismo , Hemorragia Cerebral/metabolismo , Trombina/metabolismo , Animales , Antígenos CD34/metabolismo , Edema Encefálico/patología , Hemorragia Cerebral/patología , Modelos Animales de Enfermedad , Hirudinas/farmacología , Leucocitos/metabolismo , Masculino , Microglía/metabolismo , Microvasos/metabolismo , Ratas , Ratas Sprague-Dawley , Trombina/antagonistas & inhibidores
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