RESUMEN
Traditional Chinese medicine has great potential to improve wound healing. ANBP, the mixture of 4 Chinese herbs- Agrimoniapilosa, Nelumbonucifera, Boswelliacarteri, and Pollen typhae-is effective in trauma treatment while its mechanism is still elusive. In this study, quantitative proteomics and bioinformatics analyses were performed to decipher the possible roles of ANBP in accelerated wound healing of mouse skin. Among all 3171 identified proteins, 90, 71, 80, and 140 proteins were found to be differently expressed in 6 hours, 3 days, 7 days, and 14 days ANBP-treated tissues compared with corresponding control tissues, respectively. The result showed that different biological processes and pathways were activated at different healing stages. At the early healing stage, ANBP treatment mainly affected several biological processes, including immune and defense response, vascular system restoration, hemostasis and coagulation regulation, lipid metabolism and signal transduction, while muscle tissue, hair, epidermis, extracellular matrix and tissue remodeling related activities were the major events in ANBP promoted later wound healing. This is the first quantitative proteome study of ANBP-treated wound tissues, which provide a new perspective for the mechanism of ANBP accelerated wound healing and is of guiding significance for clinical application of ANBP in trauma disorders cure.
Asunto(s)
Medicamentos Herbarios Chinos/uso terapéutico , Proteómica , Cicatrización de Heridas/efectos de los fármacos , Heridas y Lesiones/tratamiento farmacológico , Heridas y Lesiones/patología , Animales , Biopsia con Aguja , Modelos Animales de Enfermedad , Inmunohistoquímica , Masculino , Ratones , Ratones Endogámicos C57BL , Distribución Aleatoria , Valores de Referencia , Sensibilidad y Especificidad , Piel/efectos de los fármacos , Piel/patología , Cicatrización de Heridas/genética , Heridas y Lesiones/genéticaRESUMEN
The diastereoselectively switchable enantioselective Mannich reaction of isatin imines with hydroxyacetone is reported. The chiral primary amino acid catalyzed this Mannich reaction to afford both anti- and syn-Mannich adducts in high yields, good diastereoselectivities, and enantioselectivities. The reason for the solvent control of the diastereoselectivity phenomenon was investigated.
Asunto(s)
Acetona/análogos & derivados , Iminas/química , Indoles/síntesis química , Isatina/química , Solventes/química , Acetona/química , Catálisis , Indoles/química , Estructura Molecular , Oxindoles , EstereoisomerismoRESUMEN
In the title compound, C(23)H(19)NO(3), there is an intra-molecular O-Hâ¯N hydrogen bond, which forms a six-membered ring, and inter-molecular O-Hâ¯O hydrogen bonds stabilize the crystal structure.
RESUMEN
In the title compound, C(24)H(21)NO(2)·C(2)H(6)O, the dihedral angle between the two aromatic ring systems is 87.00â (6)°. There is an intra-molecular O-Hâ¯N hydrogen bond, which forms a six-membered ring. Inter-molecular O-Hâ¯O hydrogen bonds stabilize the crystal structure.