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OBJECTIVE: Deep learning algorithms were used to develop a model for predicting the staging and grading of renal clear cell carcinoma to inform clinicians' treatment plans. METHODS: Clinical and pathological information was collected from 878 patients diagnosed with renal clear cell carcinoma in the Department of Urology, Peking University First Hospital. The patients were randomly assigned to the test set (n = 702) or the verification set (n = 176). Pathological staging and grading of renal clear cell carcinoma were predicted by preoperative clinical variables using deep learning algorithms. Receiver operating characteristic curves were used to evaluate the predictive accuracy as measured by the area under the receiver operating characteristic curve (AUC). RESULTS: For tumor pathological staging, AUC values of 0.933, 0.947, and 0.948 were obtained using the BiLSTM, CNN-BiLSTM, and CNN-BiGRU models, respectively. For tumor pathological grading, the AUC values were 0.754, 0.720, and 0.770, respectively. CONCLUSIONS: The proposed model for predicting renal clear cell carcinoma allows for accurate projection of the staging and grading of renal clear cell carcinoma and helps clinicians optimize individual treatment plans.
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Carcinoma de Células Renales , Aprendizaje Profundo , Neoplasias Renales , Humanos , Carcinoma de Células Renales/patología , Algoritmos , Curva ROC , Neoplasias Renales/patologíaRESUMEN
Objective To analyze the clinical features,pathogenic factors and sites of thrombus involvement in patients with cerebral venous/venous sinus thrombosis (CVST) complicated with cerebral hemorrhage.Methods Eighty-seven patients with CVST,admitted to our hospital from January 2013 to December 2016,were selected and divided into observation group (n=39) and control group (n=48) according to cerebral hemorrhage.The demographic data,clinical features,pathogenic factors,and location of the involved venous/venous sinus were compared and analyzed between the two groups.The independent influencing factors of CVST combined with intracerebral hemorrhage were assessed using multivariable Logistic regression.Results Percentages of patients with decreased visual acuity (35.9％),epileptic seizure (48.7％),motor/sensory disorders (46.2％),consciousness changes (25.6％) and aphasia (12.8％) of the observation group were significantly higher than those of the control group (12.5％,16.7％,18.8％,8.3％,and 0％,P＜0.05).In the aspect of potential pathogenic factors,the proportion of patients at pregnancy/puerperium in the observation group (20.6％) was significantly higher than that in the control group (6.3％,P＜0.05).In the aspect of involving venous/venous sinus area,the proportion of patients involved in straight sinus in the observation group (30.8％) was significantly higher than that in the control group (12.5％,P＜0.05).Multivariate Logistic analysis showed that pregnancy/puerperium and involvement of straight sinus were the independent influencing factors of CVST combined with cerebral hemorrhage (OR=6.752,P=0.017,95％CI:2.295-16.213;OR=4.573,P=0.029,95％CI:1.316-11.751).Conclusion CVST patients at pregnancy/postpartum or with straight sinus thrombosis are more prone to cerebral hemorrhage,and targeted treatment should be given as soon as possible.
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Objective To investigate the effect of human urinary kallidinogenase (HUK) on cerebral ischemia reperfusion injury in mice.Methods One hundred and ten male ICR mice were randomly divided into sham operation,control and HUK groups.A cerebral ischemia-reperfusion model was induced by transient middle cerebral artery occlusion.The infarct volume was detected by triphenyltetrazolium chloride staining.Bcl-2,Bax,and caspase-3 expression levels in the ischemic cortex were detected by Western blot.Bcl-2 and Bax positive cells in the hippocampal CA1 area on the ischemic side were detected using Immunohistochemical staining.Apoptotic cells in the ischemic cortex were detected by TUNEL staining.Results No infarction and neurological deficits were found in the sham operation group.At 24 h after ischemia-reperfusion,the infarction voltne (P ＜0.01) and neurological deficit score (P =0.02) in the HUK group were significantly lower than those in the control group;at 72 h after ischemia-reperfusion,the infarction volume (P ＜ 0.01) and neurologic deficit score (P =0.03) in the HUK group were also significantly lower than those in the control group.Westem blot analysis showed that the expression level of Bcl-2 in the ischemic cortex in the HUK group was significantly higher than that in the control group (P ＜ 0.001),and the expression levels of caspase-3 (P ＜ 0.001) and Bax (P ＜ 0.001) in the cerebral cortex in the HUK group were significantly lower than those in the control group.No apoptotic cells were found in the sham operation group.The number of apoptotic cells in hippocampal CA1 area (P ＜ 0.01) and the number of Bax positive cells (P ＜0.01) in the HUK group were significantly less than those in the control group,while the number of Bcl-2 positive cells was significantly more in the control group (P ＜ 0.01).Conclusions HUK has a certain protective effect on ischemia-reperfusion injury in mice,its mechanism may be associated with the upregulation of Bcl-2 protein expression and downregulation of caspase-3 and Bax protein expression,thus inhibiting cell apoptosis.
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A total of 3696 Ixodid ticks, collected from Hebei Province, China, were examined by a nested polymerase chain reaction for the presence of Anaplasma phagocytophilum. Forty-three (15.4%) of 280 pools tested, including 39 (14.6%) of 267 Haemaphysalis longicornis and four (30.8%) of 13 Dermacentor nuttalli, were positive, but no significant difference was found between D. nuttalli and H. longicornis (p>0.05). Sequence and phylogenetic analyses of 16S rRNA gene indicated that A. phagocytophilum in China is genetically diverse. To our knowledge, this is the first evidence of A. phagocytophilum in ticks from Hebei Province, China, and the first documentation of Anaplasma infection in D. nuttalli.