RESUMEN
The aim of the study was to reveal the mastication forces effect on the microstructure of mandible bone tissue of mini-pigs by Fouirier harmonic imaging analysis of bone sections images of back scattered electrons and assessment of calcium and phosphorous distribution maps obtained by roentgenofluorescence technique. The results showed that by higher functional loads not only the total content of mineral elements in the bone matrix increased but also the of the low-frequency harmonics in the image spectrum indicating structural heterogeneity decrease in bone mineralization.
Asunto(s)
Proceso Alveolar/ultraestructura , Matriz Ósea/ultraestructura , Calcificación Fisiológica , Procesamiento de Imagen Asistido por Computador/métodos , Mandíbula/ultraestructura , Masticación , Proceso Alveolar/diagnóstico por imagen , Proceso Alveolar/metabolismo , Animales , Matriz Ósea/diagnóstico por imagen , Matriz Ósea/metabolismo , Mandíbula/diagnóstico por imagen , Mandíbula/metabolismo , Minerales/metabolismo , Radiografía , Espectrometría por Rayos X , Porcinos , Porcinos EnanosRESUMEN
The mandible alveolar bone tissue of mini-pig was evaluated by means of radiospectral analysis. Fourier analysis of mineral content distribution in bone slices showed that loss of masticatory load leads to focal demineralization. With time the demineralization foci increased in amount, not in size.
Asunto(s)
Absorciometría de Fotón/métodos , Proceso Alveolar/diagnóstico por imagen , Análisis de Fourier , Desmineralización Dental/diagnóstico por imagen , Animales , Densidad Ósea , Esmalte Dental/diagnóstico por imagen , Esmalte Dental/patología , Dentina/diagnóstico por imagen , Dentina/patología , Porcinos , Porcinos Enanos , Desmineralización Dental/patologíaRESUMEN
The destruction of human serum albumin was studied under the influence of the preparations used for chemical-mechanic removal of carious dentine (GK-101 and Caricleans, gel #2) and model systems containing sodium hypochlorite and amino compounds. Control contained hydroxide and sodium chloride but not hypochlorite. The degree of albumin destruction was determined by the amount of low molecular weight fragments precipitated by phosphotungstic acid but not by trichloracetic acid. From the results of the study the conclusion was made that in order to reduce the injuring action of hypochlorite upon dental tissues and soft tissues of oral cavity it is more preferable to use systems containing the addition of neutral and basic amino compounds.
Asunto(s)
Cloraminas/química , Caries Dental/tratamiento farmacológico , Dentina , Glicina/análogos & derivados , Aminas/química , Cloraminas/uso terapéutico , Glicina/química , Glicina/uso terapéutico , Humanos , Hidróxidos/química , Ácido Hipocloroso/química , Albúmina Sérica/química , Cloruro de Sodio/químicaRESUMEN
Study for determination of human individual sensitivity to fluoride was performed on 140 patients of CRIS clinics of the age from 20 to 65 years. It was established that even in cases of F-concentrations analogous to high physiological F-concentrations in blood serum (0.19 ppm) in 11.4% of the examined patients the signs of higher F-sensitivity were observed. Human individual sensitivity to fluoride was dependant upon the severity of patient's status. The results received can be used as a base for tactics development of timely and adequate correction of F-dosage.
Asunto(s)
Cariostáticos/administración & dosificación , Evaluación de Medicamentos/métodos , Tolerancia a Medicamentos , Fluoruros/administración & dosificación , Adulto , Anciano , Cariostáticos/análisis , Femenino , Fluoruros/sangre , Humanos , Masculino , Persona de Mediana EdadRESUMEN
Differences in the levels of tissue respiration in the rat skin flaps incubated in perfluothane (PF) and normal saline (NS) are regarded as an evidence of local PF effect on tissue substrate. The complex of metabolic changes in the skin fragments under the effect of PF in comparison with skin fragments incubated in NS indicate a protective effect of PF on tissue substrata. Decreased content of malonic dialdehyde and a slower accumulation of tissue liquid in skin flaps incubated in PF, in comparison with the fragments incubated in NS, is worthy of note. It indicates that incubation in PF (in comparison with incubation in NS) is less harmful for cell membranes and induces lesser disorders in water-salt metabolism and edema which are usually associated with tissue lesions developing under conditions of trophic deficiency.
Asunto(s)
Sustitutos Sanguíneos/farmacología , Fluorocarburos/farmacología , Piel/efectos de los fármacos , Piel/metabolismo , Colgajos Quirúrgicos , Animales , Respiración de la Célula/efectos de los fármacos , Respiración de la Célula/fisiología , Citoprotección/efectos de los fármacos , Citoprotección/fisiología , Glucólisis/efectos de los fármacos , Peroxidación de Lípido/efectos de los fármacos , Masculino , Ratas , Ratas Wistar , Colgajos Quirúrgicos/fisiología , Conservación de Tejido/métodosRESUMEN
Relationship between alkaline phosphatase immobilized on apatite and remineralization processes has been studied. The enzyme notably accelerated the remineralization. The course of enzymatic remineralization depended on the source of phosphorus (inorganic phosphorus or beta-glycerophosphate). Calcium incorporation in remineralizing surface was the most rapid in the presence of beta-glycerophosphate. This may be explained by a higher rate of formation of apatite under the effect of immobilized alkaline phosphatase, than of brushite or vitlokite.
Asunto(s)
Fosfatasa Alcalina/farmacología , Enzimas Inmovilizadas/farmacología , Saliva/enzimología , Calcificación de Dientes/efectos de los fármacos , Fosfatasa Alcalina/antagonistas & inhibidores , Apatitas , Esmalte Dental/efectos de los fármacos , Inhibidores Enzimáticos/farmacología , Enzimas Inmovilizadas/antagonistas & inhibidores , Humanos , Técnicas In Vitro , Levamisol/farmacología , Soluciones , Remineralización Dental/métodosRESUMEN
The kinetic method revealed that by the rate of adsorption on apatite, serum alkaline phosphatase (AlP) is homologous and salivary AlP consists of two fractions: "slow" with the constant of adsorption rate approximating that of serum AlP and "fast" with 5-6 times greater constant. A mechanism of phosphatase immobilization on apatite by two-stage sequential reaction is proposed. The constants of rates of both stages for the serum phosphatase and fast salivary fraction are determined. Difference of the products of both stages by the Michaelis constant (KM) is demonstrated for the fast fraction. The KM of the second-stage immobilization product is close to that of AlP immobilized on dental enamel, which confirms the hypothesis about their identity. In contrast to AlP, both serum and salivary alpha-amylase react with apatite at the same rate and, probably, by the same mechanism as the bulk of salivary and serum protein.