RESUMEN

Cellular clone (MAK 14-7), producing antibodies against the virus of Venezuela equine encephalomyelitis (VEE), strain 230, was isolated using the standard hybridomata technology. Monoclonal antibodies neutralized the viral hemagglutinating activity leaving the infectious one intact. Monoclonal antibodies from MAD 14-7 reacted specifically with viral glycoprotein E1 as registered by the immunoprecipitation technique. The topography of antigenic determinants of viral E1/E2 glycoprotein dimer forming the virions outer spikes is discussed in connection with the results obtained.

Asunto(s)

Anticuerpos Monoclonales/aislamiento & purificación , Anticuerpos Antivirales/inmunología , Virus de la Encefalitis Equina Venezolana/inmunología , Glicoproteínas/inmunología , Hemaglutininas Virales/inmunología , Animales , Anticuerpos Monoclonales/inmunología , Unión Competitiva , Línea Celular , Virus de la Encefalitis Equina Venezolana/patogenicidad , Pruebas de Hemaglutinación , Ratones , Virión/inmunología , Virión/patogenicidad