RESUMEN
This paper reports the immunohistochemical changes in kidneys of anaphylactic shock animals byAvidin-biotin-per-oxidase Complex Technique (ABC), Peroxidase - anti - perox idase Complex Technique(PAP)and Lablled Avidin-biotin Technique (LAB). The immediate naphylaxis was always accompanied by The immunocomplex formation in kidney. the immunocomplex adhered to the mesangial cells,the endothelial cells of capillaries and small arteries,and also to the adventitia of blnd vessels. In addition to allegens,such as penicillin and exogenous serum, IgE and IgG were observed in theimmuno-complex. This finding suggested that the IgE in the immunocomplex might be characteristicof type I anaphylaxis and could be distingushed from other kind of immunocomtplexs. Animals keptedat the room temperature (22 - 25℃)for 6 or 12 hours, or in refrigerator (4 ℃ )for 48 hours would notinfluence the positive reactions. This method seems feasible for practical use in the casework of anaphylactic shock.
RESUMEN
Having collected the skull of a fallen adult victim,and made artificial fractures on an autopsled adult skull as well as on two living dog skulls,we observed the edges of skull fractures under the scanning electron microscope in order to find out the submicroscopic morphologic features of skull fractures aiming at elucidating the mechanism of fracture,and differentiating ante-from post-mortem fractures.Many microfractures were found along the main fracture lines,they extended into the interior of the skull. The width of the microfractures varied from 5 to 100?m.Some of them were located in the external compact bone and some between the outer compact bone and diploe. The rest were found in the diploe in the form of transverse,longitudinal or spiral fractures of bony trabeculae.Collagenous fibre bunches embeded in the bony matrix were broken and separated. The blood vessels inside the skull were crosscut or longitudinary torn by the fracture lines. Sometimes the torn blood vessels were pulled out of the Harversian canals.In antemortem fractures as a results of hemorrhage, there were not only blood clot on the edges of the broken skull, but also hemorrhage into the microfractures. The fibrin networks and red blood cells were easily found in the depths of these cracks.A deductive mechanism of formation of the microfractures and the possibility of differentiation between ante-and post-mortem fractures are discussed.
RESUMEN
This paper reports the measurement of thromboxane B2 (TXB2) levels in guinea pig died of anaphylactic shock by the radioimmunassay test (RIST). 1. Statistically significant difference of plasma TXB2 level was existed between the control group (9.63?1.77ng/ml), test group before shock (9.26?3.01ng/ml) and the test group after shock (penicillin group 30.36?10.72ng/ml;serum group 40.10?6.51ng/ml) (P