RESUMEN

In this study, we developed a model of sperm in vitro differentiation to study the mechanism of spermatogenesis. We isolated newborn male germ cells for in vitro differentiation. We found that after 4-5 weeks of culture, sperm-like cells were occasionally observed in cell co-culture and the feed-layer. After 1-2 weeks of culture followed by 4-5 weeks of co-culture, sperm-like cells were observed.

Asunto(s)

Espermatogénesis/fisiología , Espermatozoides/citología , Animales , Animales Recién Nacidos , Bovinos , Diferenciación Celular/fisiología , Técnicas de Cocultivo/métodos , Células Germinativas , Masculino , Células de Sertoli/citología , Espermatogonias/citología , Células Madre/citología , Técnicas de Cultivo de Tejidos/métodos