RESUMEN
Quantitative magnetic resonance image (MRI) in individual muscles may be useful for monitoring disease progression in Duchenne muscular dystrophy (DMD). The purpose of this study was to measure T2 relaxation time of thigh muscles in children with DMD and healthy boys, and to correlate the T2 relaxation time of muscles with the fat fraction (FF) at quantitative magnetic resonance and results of clinical assessment. Thirty-two boys with DMD and 18 healthy boys were evaluated with T2 mapping and three-point Dixon MRI. Age, body mass index (BMI), muscle strength assessment, timed functional tests (time to walk or run 10 metres, rise from the floor and ascend four stairs), and the North Star Ambulatory Assessment (NSAA) were evaluated. Spearman's correlation was used to assess the relationships between FF and clinical assessments and T2 relaxation time. The mean T2 relaxation time of thigh muscles in DMD was significantly longer than that in the control group (P<0.05), except for the gracilis (P=0.952). The gracilis, sartorius and adductor longus were relatively spared by fatty infiltration in DMD patients. The T2 relaxation time was correlated significantly with the mean FF in all muscles. Age, BMI, total muscle strength score, timed functional tests and NSAA were significantly correlated with the overall mean T2 relaxation time. T2 mapping may prove clinically useful in monitoring muscle changes as a result of the disease process and in predicting the outcome of DMD patients.
Asunto(s)
Tejido Adiposo/diagnóstico por imagen , Músculo Esquelético/diagnóstico por imagen , Distrofia Muscular de Duchenne/diagnóstico por imagen , Muslo/diagnóstico por imagen , Adolescente , Índice de Masa Corporal , Estudios de Casos y Controles , Niño , Preescolar , Progresión de la Enfermedad , Humanos , Imagen por Resonancia Magnética , Masculino , Estudios ProspectivosRESUMEN
This study aimed to assess coronary microvascular dysfunction (CMD) differences in hypertrophic cardiomyopathy (HCM) patients using cardiac magnetic resonance (CMR) first-pass perfusion and late gadolinium enhancement imaging. Forty-seven patients with HCM and twenty-one healthy volunteers underwent CMR at rest. Imaging protocols included short axis cine, first-pass myocardial perfusion, and late gadolinium enhancement (LGE). Left ventricular end-diastolic wall thickness (EDTH), LGE, time to peak (Tpeak), maximal up-slope (Slopemax), and peak signal intensity (SIpeak) were assessed for each myocardial segment. The HCM myocardial segments were grouped by the degree of LGE and hypertrophy. Tpeak, SIpeak, Slopemax and EDTH in multiple groups were assessed and compared by ANOVA test/Kruskal-Wallis test. The Spearman correlation test was used to determine the relationships between EDTH, LGE and perfusion parameters (Tpeak, Slopemax and SIpeak). Compared to control group segments, Tpeak increased while Slopemax and SIpeak decreased in non-LGE/non-hypertrophic segments and LGE/hypertrophic segments in the HCM group, while Tpeak increased more significantly in LGE/hypertrophic segments (all p < 0.05). Tpeak statistically increased with increasing degrees of myocardial LGE (p < 0.01). Differences in Tpeak, SIpeak and EDTH were observed between segments with and without hypertrophy (p < 0.05). EDTH and LGE were positively correlated with Tpeak (r = 0.279, p = 0.031 and r = 0.237, p < 0.001). 3.0 T magnetic resonance myocardial perfusion imaging identifies abnormal perfusion in non-LGE and non-hypertrophic segments of HCM patients, and it may be helpful in the early diagnosis of coronary microvascular dysfunction in HCM. This abnormal perfusion is associated with the severity of myocardial fibrosis and the degree of hypertrophy.
Asunto(s)
Cardiomiopatía Hipertrófica/diagnóstico por imagen , Circulación Coronaria , Vasos Coronarios/diagnóstico por imagen , Imagen por Resonancia Cinemagnética , Microcirculación , Microvasos/diagnóstico por imagen , Imagen de Perfusión Miocárdica/métodos , Adulto , Anciano , Cardiomiopatía Hipertrófica/fisiopatología , Estudios de Casos y Controles , Medios de Contraste/administración & dosificación , Vasos Coronarios/fisiopatología , Femenino , Fibrosis , Gadolinio DTPA/administración & dosificación , Humanos , Interpretación de Imagen Asistida por Computador , Masculino , Microvasos/fisiopatología , Persona de Mediana Edad , Miocardio/patología , Valor Predictivo de las Pruebas , Pronóstico , Reproducibilidad de los Resultados , Índice de Severidad de la Enfermedad , Función Ventricular Izquierda , Remodelación VentricularRESUMEN
BACKGROUND: Associations between the NQO1 C609T polymorphism and hepatocellular carcinoma (HCC) risk are a subject of debate. We therefore performed the present meta-analysis to evaluate links with HCC susceptibility. MATERIALS AND METHODS: Several major databases (PubMed, EBSCO), the Chinese national knowledge infrastructure (CNKI) and the Wanfang database were searched for eligible studies. Crude odds ratios (ORs) with 95% confidence intervals (CIs) were used to measure the strength of associations. RESULTS: A total of 4 studies including 1,325 patients and 1,367 controls were identified. There was a significant association between NQO1 C609T polymorphism and HCC for all genetic models (allelic model: OR=1.45, 95%CI=1.23-1.72, p<0.01; additive model: OR=1.96, 95%CI=1.57-2.43, p<0.01; dominant model: OR=1.62, 95%CI=1.38-1.91, p<0.01; and recessive model: OR=1.53, 95%CI=1.26-1.84, p<0.01). On subgroup analysis, similarly results were identified in Asians. For Asians, the combined ORs and 95% CIs were (allelic model: OR=1.50, 95%CI=1.24-1.82, p<0.01; additive model: OR=2.11, 95%CI=1.48-3.01, p<0.01; dominant model: OR=1.69, 95%CI=1.42-2.02, p<0.01; and recessive model: OR=1.59, 95%CI=1.16-2.19, p<0.01). CONCLUSIONS: The current meta-analysis suggested that the NQO1 C609T polymorphism could be a risk factor for developing HCC, particularly in the Chinese population.
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Carcinoma Hepatocelular/genética , Predisposición Genética a la Enfermedad , Neoplasias Hepáticas/genética , NAD(P)H Deshidrogenasa (Quinona)/genética , Pueblo Asiatico/genética , Carcinoma Hepatocelular/epidemiología , China/epidemiología , Estudios de Asociación Genética , Humanos , Neoplasias Hepáticas/epidemiología , Polimorfismo de Nucleótido Simple , RiesgoRESUMEN
OBJECTIVE: To assess the value of 64-slice spiral computed tomography (MSCT) coronary angiography in patients with coronary heart disease. METHODS: Fifty-eight patients were performed 64-slice spiral CT in coronary artery. Intravenously contrast enhanced 64-slice spiral computed tomography coronary angiography was achieved during a single breath hold, and ECG images were reconstructed retrospectively. All axial images were transferred to an external workstation. The reconstructed images were processed into volume rendering (VR) and multiplanar reconstruction (MPR) and curved planner reconstruction (CPR) and maximum intensity projection (MIP), 24 of the 58 patients underwent selective coronary artery angiography, the outcomes of the two kinds of examination were compared. RESULTS: The main branches and of the sub-branches of the coronary artery were clearly displayed. 115 coronary artery branches of 38 patients were found coronary plaques, plaque morphology and stenosis extent could be clearly differentiated. In the segments with coronary stents and bypass graft, the position and distal blood flow were well showed. CONCLUSION: 64-slice MSCT coronary angiography is a reliable diagnostic method for coronary heart disease and promising technique for noninvasive screening and diagnosis of coronary artery disease.
Asunto(s)
Angiografía Coronaria/métodos , Enfermedad Coronaria/diagnóstico por imagen , Tomografía Computarizada Espiral/métodos , Angioplastia Coronaria con Balón , Enfermedad Coronaria/terapia , Humanos , Persona de Mediana EdadRESUMEN
OBJECTIVE: To establish the 3T3 mouse fibroblast neutral red uptake (NRU-PT) phototoxicity test method, and evaluate the practicality of the method in detecting potential phototoxicity of the cosmetic products. METHODS: Fifteen phototoxic and 9 non-phototoxic chemicals were tested in our laboratories, the phototoxic potential of the test chemicals was evaluated in a prediction model in which either the photo irritation factor (PIF) or the mean photo effect (MPE) was compared with the coherence and sensitivity of the method. 20 kinds of functional cosmetics were detected and the results were analyzed by the 3T3 NRU-PT in vitro and Guinea pig skin phototoxicity test (in vivo). RESULTS: Both PIF and MPE of the chemicals were highly reproduced, and the correlation between in vitro and in vivo data was almost perfect. All the non-phototoxic provided a negative result, while 14 of the 15 phototoxic tested chemicals gave clear positive results. For cosmetics, the correlation between in vitro and in vivo data was consistent. CONCLUSION: The 3T3 NRU PT test was established successfully, it should be used as a good alternative method for assessing the phototoxic potential of the chemicals and cosmetics in China.