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1.
Vet Res ; 52(1): 119, 2021 Sep 15.
Artículo en Inglés | MEDLINE | ID: mdl-34526112

RESUMEN

Congenital avian leukosis virus subgroup J (ALV-J) infection can induce persistent immunotolerance in chicken, however, the underlying mechanism remains unclear. Here, we demonstrate that congenital ALV-J infection induces the production of high-frequency and activated CD4+CD25+ Tregs that maintain persistent immunotolerance. A model of congenital infection by ALV-J was established in fertilized eggs, and hatched chicks showed persistent immunotolerance characterized by persistent viremia, immune organ dysplasia, severe imbalance of the ratio of CD4+/CD8+ T cells in blood and immune organs, and significant decrease in CD3+ T cells and Bu-1+ B cells in the spleen. Concurrently, the mRNA levels of IL-2, IL-10, and IFN-γ showed significant fluctuations in immune organs. Moreover, the frequency of CD4+CD25+ Tregs in blood and immune organs significantly increased, and the frequency of CD4+CD25+ Tregs was positively correlated with changes in ALV-J load in immune organs. Interestingly, CD4+CD25+ Tregs increased in the marginal zone of splenic nodules in ALV-J-infected chickens and dispersed to the germinal center. In addition, the proliferation and activation of B cells in splenic nodules was inhibited, and the number of IgM+ and IgG+ cells in the marginal zone significantly decreased. We further found that the mRNA levels of TGF- ß and CTLA-4 in CD4+CD25+ Tregs of ALV-J-infected chickens significantly increased. Together, high-frequency and activated CD4+CD25+ Tregs inhibited B cells functions by expressing the inhibitory cytokine TGF-ß and inhibitory surface receptor CTLA-4, thereby maintaining persistent immunotolerance in congenital ALV-J-infected chickens.


Asunto(s)
Virus de la Leucosis Aviar/inmunología , Leucosis Aviar/inmunología , Pollos , Tolerancia Inmunológica , Enfermedades de las Aves de Corral/inmunología , Linfocitos T/inmunología , Animales , Linfocitos T CD4-Positivos , Embrión de Pollo , Organismos Libres de Patógenos Específicos
2.
Vet Microbiol ; 247: 108781, 2020 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-32768227

RESUMEN

Immune tolerance induced by avian leukosis virus subgroup J (ALV-J) is a prerequisite for tumorigenesis. Although we had reported that B cell anergy induced by ALV-J was the main reason for immune tolerance, the molecular mechanism still remains unclear. Here, we found SU protein of ALV-J interacted with tyrosine kinase Lyn (a key protein in BCR signaling pathway) by confocal laser scanning microscopy and co-immunoprecipitation test, which suggested that Lyn might play an important role in B cell anergy induced by ALV-J. Correspondingly, the mRNA and protein level of Lyn was significantly up-regulated in B cells after ALV-J infection. Subsequently, the phosphorylated protein levels of Lyn at Tyr507 site were significantly up-regulated in ALV-J-infected B cells after BCR signal activation, but the phosphorylated protein level of Syk (a direct substrate of Lyn) at Tyr525/526 site, Ca2+ flux, and NF-κB p65 protein level were significantly down-regulated. Interestingly, the phosphorylated protein level of Syk at Tyr525/526 site, Ca2+ flux, and NF-κB p65 protein level were both significantly retrieved after the shLyn treatment in B cells infected by ALV-J. In summary, these results indicated that ALV-J activated the negative regulatory effect of phosphorylated Lyn protein at 507 site in BCR signal transduction pathway and then mediated B cell anergy, which will provide a new insight for revealing the pathogenesis of immune tolerance induced by ALV-J.


Asunto(s)
Virus de la Leucosis Aviar/inmunología , Linfocitos B/inmunología , Anergia Clonal , Transducción de Señal/inmunología , Familia-src Quinasas/genética , Animales , Leucosis Aviar/inmunología , Leucosis Aviar/virología , Virus de la Leucosis Aviar/clasificación , Linfocitos B/virología , Pollos/inmunología , Pollos/virología , Regulación de la Expresión Génica , Fosforilación , Enfermedades de las Aves de Corral/virología , Organismos Libres de Patógenos Específicos , Regulación hacia Arriba
3.
Virus Res ; 264: 32-39, 2019 04 15.
Artículo en Inglés | MEDLINE | ID: mdl-30797826

RESUMEN

The lifecycle of avian leukosis virus subgroup J (ALV-J), a typical tumorigenic retrovirus, is highly dependent upon host cellular proteins. However, there have been few studies directed at uncovering the host proteins responsible for ALV-J replication, which could provide insights into new strategies for ALV-J prevention and control. Here, we used proteomics to identify the association of differential levels of collagen triple helix-repeat-containing 1 (CTHRC1) and with viral replication. Our results revealed that CTHRC1 was significantly upregulated in ALV-J-infected cells in vitro, and these findings were confirmed in vivo. Additionally, CTHRC1 overexpression facilitated ALV-J replication, whereas CTHRC1 knockdown suppressed this activity. Moreover, we found that ALV-J drove CTHRC1 translocation from the nucleus to the cytosol through interactions with the ALV-J envelope glycoprotein. These results revealed CTHRC1 as a shutting protein is recruited by ALV-J to facilitate viral replication.


Asunto(s)
Virus de la Leucosis Aviar/fisiología , Proteínas de la Matriz Extracelular/genética , Interacciones Microbiota-Huesped , Proteínas del Envoltorio Viral/genética , Replicación Viral , Animales , Línea Celular , Embrión de Pollo , Pollos/virología , Proteínas de la Matriz Extracelular/metabolismo , Fibroblastos/virología , Proteómica , Proteínas del Envoltorio Viral/metabolismo
4.
Retrovirology ; 16(1): 1, 2019 01 03.
Artículo en Inglés | MEDLINE | ID: mdl-30602379

RESUMEN

BACKGROUND: The pathogenesis of immunological tolerance caused by avian leukosis virus subgroup J (ALV-J), an oncogenic retrovirus, is largely unknown. RESULTS: In this study, the development, differentiation, and immunological capability of B cells and their progenitors infected with ALV-J were studied both morphologically and functionally by using a model of ALV-J congenital infection. Compared with posthatch infection, congenital infection of ALV-J resulted in severe immunological tolerance, which was identified as the absence of detectable specific antivirus antibodies. In congenitally infected chickens, immune organs, particularly the bursa of Fabricius, were poorly developed. Moreover, IgM-and IgG-positive cells and total immunoglobulin levels were significantly decreased in these chickens. Large numbers of bursa follicles with no differentiation into cortex and medulla indicated that B cell development was arrested at the early stage. Flow cytometry analysis further confirmed that ALV-J blocked the differentiation of CD117+chB6+ B cell progenitors in the bursa of Fabricius. Furthermore, both the humoral immunity and the immunological capability of B cells and their progenitors were significantly suppressed, as assessed by (a) the antibody titres against sheep red blood cells and the Marek's disease virus attenuated serotype 1 vaccine; (b) the proliferative response of B cells against thymus-independent antigen lipopolysaccharide (LPS) in the spleen germinal centres; and (c) the capacities for proliferation, differentiation and immunoglobulin gene class-switch recombination of B cell progenitors in response to LPS and interleukin-4(IL-4) in vitro. CONCLUSIONS: These findings suggested that the anergy of B cells in congenitally infected chickens is caused by the developmental arrest and dysfunction of B cell progenitors, which is an important factor for the immunological tolerance induced by ALV-J.


Asunto(s)
Virus de la Leucosis Aviar/inmunología , Leucosis Aviar/congénito , Subgrupos de Linfocitos B/patología , Anergia Clonal , Enfermedades de las Aves de Corral/congénito , Células Madre/patología , Animales , Anticuerpos Antivirales/sangre , Leucosis Aviar/patología , Virus de la Leucosis Aviar/patogenicidad , Subgrupos de Linfocitos B/química , Subgrupos de Linfocitos B/inmunología , Subgrupos de Linfocitos B/virología , Bolsa de Fabricio/patología , Diferenciación Celular , Proliferación Celular , Pollos , Citometría de Flujo , Inmunoglobulina G/sangre , Inmunoglobulina M/sangre , Enfermedades de las Aves de Corral/patología , Proteínas Proto-Oncogénicas c-kit/análisis , Células Madre/química , Células Madre/inmunología , Células Madre/virología
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