RESUMEN

Mesenchymal stem cells (MSCs) are one of the most promising cell populations for tissue engineering and regenerative medicine. Of utmost importance to MSC research is identification of MSC sources that are easily obtainable and stable. Several studies have shown that MSCs can be isolated from amniotic fluid. The sheep is one of the main types of farm animal, and it has many biophysical and biochemical similarities to humans. Here, we obtained MSCs from ovine amniotic fluid and determined the expansion capacity, surface and intracellular marker expression, karyotype, and multilineage differentiation ability of these ovine amniotic fluid mesenchymal stem cells (oAF-MSCs). Moreover, expression levels of differentiation markers were measured using reverse transcription-qPCR (RT-qPCR). Our phenotypic analysis shows that the isolated oAF-MSCs are indeed MSCs.

Asunto(s)

Líquido Amniótico/citología , Separación Celular/métodos , Células Madre Mesenquimatosas , Adipogénesis , Animales , Antígenos CD/análisis , Antígenos de Superficie/análisis , Diferenciación Celular/genética , Células Cultivadas , Condrogénesis , Femenino , Humanos , Cariotipo , Células Madre Mesenquimatosas/citología , Células Madre Mesenquimatosas/metabolismo , Osteogénesis , Fenotipo , Embarazo , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Ovinos