RESUMEN
PURPOSE: To compare the biological behavior of mouse osteoblast-like cells (MC3T3-E1) on hydroxyapatite (HA)-coated nanotube surface of titanium and plasma-sprayed HA (HA-PS)-coated titanium surface. MATERIALS AND METHODS: The HA-coated nanotube surface of titanium were fabricated by anodization coupled with alternative immersion method (AIM). MC3T3-E1 osteoblast cells cultured in vitro were seeded onto these different surfaces; their growth states were examined by a confocal laser scanning microscope; the proliferation behavior, alkaline phosphatase (ALP) activity, osteocalcin (OCN) secretion, and analysis of osteoblastic gene expressions were also compared in detail. RESULTS: Significant increases in ALP activity and OCN production on days 7 and 14 (P < 0.05) were observed for AIM-coated HA (HA-AIM) surfaces. However, cells cultured on HA-AIM-coated surfaces showed a delayed growth pattern. Real-time polymerase chain reaction analyses showed significantly higher relative mRNA expression levels of osteoblastic genes (runt-related protein 2, osterix, osteopontin, OCN) in cells cultured on the HA-AIM-coated nanotube surfaces as compared with cells cultured on the HA-PS and baer Ti surfaces. CONCLUSION: The current research showed that the HA-AIM-coated nanotubular Ti surfaces enhance osteoblast differentiation, which had the potential to further improve osseointegration.
Asunto(s)
Durapatita/uso terapéutico , Nanotubos/química , Osteoblastos/fisiología , Titanio , Fosfatasa Alcalina/metabolismo , Animales , Proliferación Celular , Supervivencia Celular , Células Cultivadas , Durapatita/química , Técnicas In Vitro , Ratones , Microscopía Confocal , Osteoblastos/metabolismo , Osteocalcina/biosíntesis , Reacción en Cadena en Tiempo Real de la Polimerasa , TranscriptomaRESUMEN
The modification on the titanium (Ti) implant surface is an effective method to improve the bioactivity of Ti. In this study, a hydroxyapatite (HA)-deposited nanotubular Ti surface was prepared by anodization coupled with an alternative immersion method (AIM). Surface physicochemical characteristics including morphology, microstructure, composition, roughness were evaluated by field-emission scanning electron microscopy (FE-SEM), X-ray diffraction (XRD), Fourier-transformed infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). Preosteoblast cell (MC3T3-E1) behavior on the HA-deposited nanotube surface, including cell morphology, proliferation, alkaline phosphatase (ALP) activity, osteocalcin secretion, and mineralization, were also investigated. The untreated nanotube surface and bare Ti were used as controls. The results showed that synthetic HA crystals could be efficiently grown on/inside the nanotubes after AIM treatment in saturated Ca(OH)(2) and 0.02M (NH(4))(2) HPO(4). The amount of synthetic HA on nanotube layer was dependent on the number of dipping cycles. Significant increases in ALP activity and osteocalcin production on day 7 and 14 and calcium deposition on day 21 (P < 0.05) were observed for HA-deposited nanotubular Ti surface when compared with untreated nanotube layer and bare Ti. However, the cell proliferation rate on HA-deposited nanotube surfaces was slowed down significantly (P < 0.05). All these results indicated that this HA-deposited nanotube surface might have the potential benefit to enhance implant osseointegration. The synthetic HA TiO(2) nanotube loading might be a highly promising path to improve the bioactivity of Ti-based implants.
Asunto(s)
Materiales Biocompatibles Revestidos/química , Durapatita/química , Ensayo de Materiales , Nanotubos/química , Osteoblastos/metabolismo , Titanio/química , Fosfatasa Alcalina/metabolismo , Animales , Hidróxido de Calcio/química , Línea Celular , Proliferación Celular , Ratones , Osteoblastos/citología , Osteocalcina/metabolismo , Fosfatos/química , Propiedades de Superficie , Factores de TiempoRESUMEN
OBJECTIVE: To study the prevalence of food intolerance among attendants in a general hospital and to learn its related determinants in Beijing, China. METHODS: An cross sectional survey, from August 1st, 2008 to June 30th, 2009, was carried out, including 12 766 adults from Health Sciences Center, Chinese PLA General Hospital. Data for epidemiological analysis were entered (double entry) into computer and organized by EpiData 3.0. Non-conditional logistic regression model was used for odd ratio (OR) and 95%CI calculation, with statistic analysis through SPSS 13.0. RESULTS: The three leading intolerance food items were egg, crab and milk, and the prevalence rates were 28.5%, 24.5% and 24.5% respectively. Prevalence of food intolerance increased along with aging. Levels and the kinds of food intolerance were associated with age, sex and appeared a linear correlation tendency (P < 0.05). Prevalence of food intolerance in females (67.3%) was higher than that in males (56.2%). Data from multiple regression analysis showed that, comparing with age group under 40 and 40 years, the OR (95%CI) of age group 41 - 50, 51 - 60, and above 60 were 1.125 (1.027 - 1.233), 1.307 (1.176 - 1.452) and 1.536 (1.275 - 1.849) respectively. Compared to males, the OR (95%CI) of females was 1.602 (1.475 - 1.741). When compared with normal weights, the OR (95%CI) of people with low weight was 1.772 (1.207 - 2.602). CONCLUSION: Food intolerance was associated with age, sex and body mass index. Health education should be carried out according to the related characteristics of age and sex. Suggesting that reducing the risk of food intolerance, keeping the suitable weight was necessary.