RESUMEN

BACKGROUND: Bacteriophages (phages) are widespread in the environment and play a crucial role in the evolution of their bacterial hosts and the emergence of new pathogens. RESULTS: LSB-1, a reference coliphage strain, was classified as a member of the Podoviridae family with a cystic form (50 ± 5 nm diameter) and short tail (60 ± 5 nm long). The double stranded DNA was about 30 kilobase pairs in length. We identified its host range and determined the gp17 sequences and protein structure using shotgun analysis and bioinformatics technology. CONCLUSIONS: Coliphage LSB-1 possesses a tailspike protein with endosialidase activity which is probably responsible for its specific enteroinvasive E.coli host range within the laboratory.

Asunto(s)

Colifagos/genética , Colifagos/fisiología , Especificidad del Huésped , Podoviridae/genética , Podoviridae/fisiología , Secuencia de Aminoácidos , Colifagos/clasificación , Colifagos/ultraestructura , Biología Computacional , ADN Viral/química , ADN Viral/genética , Escherichia coli/virología , Modelos Moleculares , Datos de Secuencia Molecular , Filogenia , Podoviridae/clasificación , Podoviridae/ultraestructura , Estructura Secundaria de Proteína , Estructura Terciaria de Proteína , Análisis de Secuencia de ADN , Proteínas Virales/genética , Virión/ultraestructura