RESUMEN
Ischemia-reperfusion injury (IRI) has brought attention to flap failure in reconstructive surgery. To improve the prognosis of skin transplantation, we performed experimental IRI by surgical obstruction of blood flow and used sodium ferulate (SF) to prevent IRI in rats. After SF treatment, the morphological and histological changes of the skin flaps were observed by H&E and Masson's trichrome staining. We also detected the expression levels of COX-1, HO-1, and Ki67 by immunohistochemical and western blot analysis. Moreover, enzyme-linked immunosorbent assay was used to identify the content of tumor necrosis factor (TNF)-α, myeloperoxidase (MPO), malondialdehyde (MDA), and nitric oxide (NO) in peripheral blood and skin tissue. Compared with the model group, SF treatment significantly improved the recovered flap area (%) and promoted collagen synthesis. Cyclooxygenase-2 (COX-2) expression was significantly inhibited by heme oxygenase-1 (HO-1) induction after SF treatment. Furthermore, SF significantly inhibited the levels of TNF-α in peripheral blood, MPO and MDA in the skin tissue, and the increased synthesis of NO. Our results showed the protective effects of SF on IRI after flap transplantation and we believe that the protective effects of SF was closely related to the alleviation of the inflammatory response and the inhibition of the oxidative stress injury.
Asunto(s)
Estrés Oxidativo , Daño por Reperfusión , Animales , Antiinflamatorios/farmacología , Ácidos Cumáricos/farmacología , Ratas , Daño por Reperfusión/tratamiento farmacológico , Daño por Reperfusión/prevención & controlRESUMEN
Ischemia-reperfusion injury (IRI) has brought attention to flap failure in reconstructive surgery. To improve the prognosis of skin transplantation, we performed experimental IRI by surgical obstruction of blood flow and used sodium ferulate (SF) to prevent IRI in rats. After SF treatment, the morphological and histological changes of the skin flaps were observed by H&E and Masson's trichrome staining. We also detected the expression levels of COX-1, HO-1, and Ki67 by immunohistochemical and western blot analysis. Moreover, enzyme-linked immunosorbent assay was used to identify the content of tumor necrosis factor (TNF)-α, myeloperoxidase (MPO), malondialdehyde (MDA), and nitric oxide (NO) in peripheral blood and skin tissue. Compared with the model group, SF treatment significantly improved the recovered flap area (%) and promoted collagen synthesis. Cyclooxygenase-2 (COX-2) expression was significantly inhibited by heme oxygenase-1 (HO-1) induction after SF treatment. Furthermore, SF significantly inhibited the levels of TNF-α in peripheral blood, MPO and MDA in the skin tissue, and the increased synthesis of NO. Our results showed the protective effects of SF on IRI after flap transplantation and we believe that the protective effects of SF was closely related to the alleviation of the inflammatory response and the inhibition of the oxidative stress injury.
Asunto(s)
Animales , Ratas , Daño por Reperfusión/prevención & control , Daño por Reperfusión/tratamiento farmacológico , Estrés Oxidativo , Ácidos Cumáricos/farmacología , Antiinflamatorios/farmacologíaRESUMEN
The envelope protein (Env) of the Jaagsiekte sheep retrovirus (JSRV) is known to be a unique oncoprotein responsible for inducing ovine pulmonary adenocarcinoma (OPA). The objective of this study was to prepare a specific monoclonal antibody (mAb) against the JSRV Env protein using bioinformatic analysis. According to the structure and epitope prediction results of JSRV Env, the JSRV-Env572-615 antigen was prepared via peptide synthesis (amino acid sequence 572-615, denoted as JSRV-Env572-615). BALB/c mice were immunized to prepare the anti-JSRV-Env572-615 mAb. Spleen cells were fused with SP2/0 myeloma cells after being screened by indirect ELISA and cloned by limiting dilution. The specificity of mAb was evaluated by western blot analysis and immunohistochemistry assays. Western blot results showed that the JSRV Env protein was able to bind to mAb with high specificity. Immunohistochemistry assays demonstrated that the mAb was able to recognize JSRV Env in adenomatous hyperplasia of the lung. Furthermore, JSRV was detected in peripheral blood leukocytes during the pre-clinical period of OPA in 2 of the 25 sheep using this newly synthesized mAb. Therefore, this mAb may be a useful tool for the detection of JSRV in sheep.
Asunto(s)
Adenocarcinoma/diagnóstico , Adenocarcinoma/veterinaria , Anticuerpos Monoclonales/biosíntesis , Anticuerpos Antivirales/biosíntesis , Retrovirus Ovino Jaagsiekte/inmunología , Neoplasias Pulmonares/diagnóstico , Neoplasias Pulmonares/veterinaria , Adenomatosis Pulmonar Ovina/diagnóstico , Adenocarcinoma/inmunología , Adenocarcinoma/virología , Adenocarcinoma del Pulmón , Secuencia de Aminoácidos , Animales , Anticuerpos Monoclonales/química , Anticuerpos Monoclonales/aislamiento & purificación , Anticuerpos Antivirales/química , Anticuerpos Antivirales/aislamiento & purificación , Especificidad de Anticuerpos , Biología Computacional , Diagnóstico Precoz , Epítopos/química , Epítopos/inmunología , Productos del Gen env/química , Productos del Gen env/inmunología , Retrovirus Ovino Jaagsiekte/aislamiento & purificación , Leucocitos Mononucleares/inmunología , Leucocitos Mononucleares/virología , Pulmón/inmunología , Pulmón/virología , Neoplasias Pulmonares/inmunología , Neoplasias Pulmonares/virología , Ratones , Ratones Endogámicos BALB C , Péptidos/administración & dosificación , Péptidos/síntesis química , Péptidos/inmunología , Adenomatosis Pulmonar Ovina/inmunología , Adenomatosis Pulmonar Ovina/virología , Ovinos , Oveja Doméstica , Bazo/citología , Bazo/inmunologíaRESUMEN
Congenital heart disease in children is a type of birth defect. Previous studies have suggested that the transcription factor, TBX20, is involved in the occurrence and development of congenital heart disease in children; however, the specific regulatory mechanisms are yet to be evaluated. Hence, this study aimed to evaluate the relationship between the TBX20 polymorphism and the occurrence and development of congenital heart disease. The TBX20 gene sequence was obtained from the NCBI database and the polymorphic locus candidate was predicted. Thereafter, the specific gene primers were designed for the restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) of DNA extracted from the blood of 80 patients with congenital heart disease and 80 controls. The results of the PCR were subjected to correlation analysis to identify the differences between the amplicons and to determine the relationship between the TBX20 gene polymorphism and congenital heart disease. One of the single nucleotide polymorphic locus was found to be rs3999950: c.774T>C (Ala265Ala). The TC genotype frequency in the patients was higher than that in the controls, similar to that for the C locus. The odds ratio of the TC genotypes was above 1, indicating that the presence of the TC genotype increases the incidence of congenital heart diseases. Thus, rs3999950 may be associated with congenital heart disease, and TBX20 may predispose children to the defect.
Asunto(s)
Cardiopatías Congénitas/genética , Polimorfismo de Nucleótido Simple , Proteínas de Dominio T Box/genética , Adolescente , Estudios de Casos y Controles , Niño , Femenino , Humanos , MasculinoRESUMEN
To investigate the role of T-helper cells/Treg (Th17/Treg) and morbidity factors related to primary nephritic syndrome (PNS) in children, as well as the influence of ox-low density lipoprotein (ox-LDL) on Th17/Treg expression in children with PNS. To clarify the pathogenesis of PNS in children, 50 children with PNS treated in our hospital were enrolled in the study group. Additionally, 20 healthy children who came to our hospital for physical examination during the same period were enrolled in the control group. Th17 and Treg cells in children belonging to the two groups were detected by flow cytometry; the numbers of Th17/Treg cells in peripheral blood mononuclear cells at different concentrations of ox-LDL were detected simultaneously. Ox-LDL can affect the number of Th17/Treg cells in peripheral blood mononuclear cells, and both cell types decreased with increasing concentration of ox-LDL, with the numbers being significantly lower in the control group. However, the decrease in the number of Th17 cells was statistically insignificant (P > 0.05), whereas the decrease in Treg cells was more obvious and statistically significant (P < 0.05). The effect of ox-LDL the number of Treg cells was stronger than that on Th17 cells. We concluded that the imbalance of Th17/Treg cells influenced by high and low ox-LDL concentrations in children with PNS might be the immunological basis of the disease.
Asunto(s)
Lipoproteínas LDL/sangre , Síndrome Nefrótico/inmunología , Linfocitos T Reguladores/inmunología , Células Th17/inmunología , Recuento de Células , Preescolar , Femenino , Citometría de Flujo , Humanos , Leucocitos Mononucleares/inmunología , Leucocitos Mononucleares/metabolismo , Lipoproteínas LDL/inmunología , Masculino , Síndrome Nefrótico/sangre , Síndrome Nefrótico/patología , Linfocitos T Reguladores/metabolismo , Células Th17/metabolismoRESUMEN
BAK1 is a co-receptor of BRI1 in early signaling pathways mediated by brassinosteroids (BRs) and is thought to play a major role in plant growth and development. As the role of BAK1 has not yet been fully elucidated then further research is required to explore its potential for use in genetic modification to improve crops. In this study, three BAK1 genes from the amphidiploid species Brassica rapa were isolated and their kinase functions were predicted following DNA sequence analysis. A bioinformatic analysis revealed that two genes, BrBAK1-1 and BrBAK1-8, shared a conserved kinase domain and 5 tandem leucine-rich repeats (LRRs) that are characteristic of a BAK1 receptor for BR perception, whereas the third gene, BrBAK1-3, was deficient for a signal peptide, but had 4 leucine zippers and 3 leucine-rich repeats (LRRs) in an extracellular domain. All three BrBAK1 kinases localized on the cellular membrane. Ectopic expression of each BrBAK1 gene in BR-insensitive (bri1-5 mutant) Arabidopsis plants indicated that BrBAK1-1 and BrBAK1-8 were functional homologues of AtBAK1 based on the rescue of growth in the bri1-5 mutant. Overexpression of BrBAK1-3 caused a severe dwarf phenotype resembling the phenotype of null BRI1 alleles. The results here suggest there are significant differences among the three BrBAK1 kinases for their effects on plant architecture. This conclusion has important implications for genetic modification of B. rapa.
Asunto(s)
Proteínas de Arabidopsis/genética , Brassica rapa/genética , Proteínas Serina-Treonina Quinasas/genética , Arabidopsis/genética , Arabidopsis/crecimiento & desarrollo , Proteínas de Arabidopsis/biosíntesis , Brassica rapa/anatomía & histología , Brassica rapa/crecimiento & desarrollo , Regulación de la Expresión Génica de las Plantas , Mutación , Fenotipo , Fosforilación , Plantas Modificadas Genéticamente , Proteínas Serina-Treonina Quinasas/biosíntesis , Transducción de SeñalRESUMEN
The aim of this study was to investigate the expression of miR-21 in esophageal cancer and the impact of miR-21 on apoptosis, invasion, and the expression of target genes in esophageal cancer cells. Fluorescence quantitative polymerase chain reaction analysis was used to detect the expression of miR-21 in human esophageal tissues, adjacent tissues, and an esophageal cancer cell line (TE-13). The antisense miR-21 oligonucleotide was generated commercially using the solid-phase chemical synthesis method. Transient transfection was used to transfect esophageal cancer cells (TE-13 antisense and TE-13 control cells). Flow cytometry and Transwell cell assays were used to detect the apoptosis and invasion of esophageal cancer cells, respectively. The western blot method was used to detect the expression of PTEN, PDCD4, and K-ras proteins. These analyses determined that mir-21 expression significantly increased in esophageal cancer tissues and in TE-13 cells, and that this phenomenon was not associated with staging or lymph node metastasis. The apoptosis rate of TE-13 control cells was lower than that of antisense TE-13 cells indicating an enhanced invasive ability. In tissues adjacent to esophageal cancer and in TE-13 antisense cells, the expression of PTEN and PDCD4 was found to be higher than that in the control group, whereas the expression of K-ras showed the opposite pattern. Together, these results suggest that miR- 21 might be involved in the development and metastasis of esophageal cancer, through interaction with its PDCD4 and K-ras target genes.
Asunto(s)
Carcinoma de Células Escamosas/genética , Neoplasias Esofágicas/genética , MicroARNs/biosíntesis , Anciano , Apoptosis/genética , Proteínas Reguladoras de la Apoptosis/biosíntesis , Carcinoma de Células Escamosas/metabolismo , Carcinoma de Células Escamosas/patología , Línea Celular Tumoral , Proliferación Celular/genética , Regulación hacia Abajo , Neoplasias Esofágicas/metabolismo , Neoplasias Esofágicas/patología , Femenino , Humanos , Masculino , MicroARNs/genética , Persona de Mediana Edad , Invasividad Neoplásica , Oligonucleótidos Antisentido/administración & dosificación , Oligonucleótidos Antisentido/genética , Fosfohidrolasa PTEN/biosíntesis , Proteínas de Unión al ARN/biosíntesis , Transfección , Proteínas ras/biosíntesisRESUMEN
The present study aimed to explore the relationship between miRNA expression and survival in patients with esophageal cancer (EC) using meta-analysis. We searched PubMed, EMBASE, CNKI, Wanfang, and ISI Web of Science databases without time restrictions, and extracted relevant data, such as the name of first author, publication year, age, gender, number of case, etc. from the studies included. We calculated the pooled hazard ratios (HRs) using the RevMan 5.2 software. A total of five studies involving 504 subjects were included in the meta-analysis, with the purpose of analyzing the association of miRNA-21 expression with EC prognosis. The pooled HR of elevated versus decreased miR-21 expression in EC was 1.87 [95% confidence interval (CI): 1.37-2.55, P < 0.001], with elevated miR-21 expression being associated with poorer prognosis for patients with EC. Our results support a prognostic role for miR-21 in EC.
Asunto(s)
Neoplasias Esofágicas/genética , MicroARNs/biosíntesis , Pronóstico , Bases de Datos Factuales , Neoplasias Esofágicas/patología , Regulación Neoplásica de la Expresión Génica , Humanos , MicroARNs/genética , PubMed , Programas InformáticosRESUMEN
The present study aimed to investigate the effects of the rs3795879 polymorphism of the SERPINE2 gene on the development of chronic obstructive pulmonary disease (COPD) based on a systematic meta-analysis. An extensive literature search was performed to retrieve previously published case-control studies on the polymorphisms of SERPINE2 in COPD patients. Odds ratios (ORs) with 95% confidence intervals (CIs) were used to assess the association between SERPINE2 polymorphisms and risk of developing COPD. A total of 5 studies including 3034 COPD cases and 3068 controls were incorporated in the present meta-analysis. Generally, no significant association was identified between the rs3795879 polymorphism of SERPINE2 and the risk of developing COPD (G allele vs A allele: OR = 1.23, 95%CI = 0.97-1.32; GG vs GA: OR = 1.19, 95%CI = 0.81-1.76; GG vs AA: OR = 1.23, 95%CI = 0.89-1.70; dominant model: OR = 1.18, 95%CI = 0.85- 1.62; recessive model: OR = 1.19, 95%CI = 0.85-1.66). In addition, subgroup analyses according to different ethnicities and the source of controls suggest no statistically significant association between the rs3795879 polymorphism of SERPINE2 and COPD risk. The results suggest that the rs3795879 polymorphism of SERPINE2 is not a risk factor for COPD.
Asunto(s)
Estudios de Asociación Genética , Predisposición Genética a la Enfermedad , Polimorfismo de Nucleótido Simple/genética , Enfermedad Pulmonar Obstructiva Crónica/genética , Serpina E2/genética , Intervalos de Confianza , Etnicidad/genética , Heterogeneidad Genética , Humanos , Oportunidad Relativa , Sesgo de PublicaciónRESUMEN
The purpose of this study was to analyze the therapy of a severe abdominal compartment syndrome (ACS) to elucidate the use of an abdominal advanced flap with other supportive measures for restoration of large defects of the abdominal wall. A patient presented with a large defect of the abdominal wall caused by ACS, which had resulted from multiple injuries after a fall from height. Healing of the defect was achieved by transplantation of an abdominal advanced flap and other supportive strategies. All of the treatment measures are presented to demonstrate complicated treatment procedures for closure of large defects of the abdominal wall. An abdominal advanced flap combined with other supportive measures was successfully applied in the healing process of ACS. This study examined the treatment of a case of ACS caused by severe abdominal trauma. The results demonstrated that a large defect of the abdominal wall caused by ACS should be closed as early as possible, and an abdominal advanced flap combined with complex supportive measures can be a recommended strategy for closing large defects of the abdominal wall.
Asunto(s)
Traumatismos Abdominales/cirugía , Hipertensión Intraabdominal/cirugía , Colgajos Quirúrgicos , Drenaje , Humanos , Masculino , Persona de Mediana Edad , Cicatrización de HeridasRESUMEN
The aim of the current study was to investigate the effects of Yes-associated protein 1 (YAP1) gene expression after birth on the development of muscle and the relationship between YAP1 and myostatin (MSTN) and myogenin (MyoG). Reverse transcription polymerase chain reaction was used to analyze the trends in YAP1, MSTN, and MyoG temporal and spatial expression levels in various skeletal muscles (i.e., longissimus dorsi muscle, soleus muscle, gastrocnemius muscle, and extensor digitorum longus) and across 3 different growth stages (i.e., 2 days old, 2 and 6 months old) of Hu Sheep. The results showed that YAP1 expression was significantly different in the skeletal muscles of sheep; the expression level gradually increased with age; it was highly expressed in the gastrocnemius muscle and minimally expressed in the longissimus dorsi muscle. MSTN, a negative regulator of skeletal muscle development, was minimally expressed in the soleus muscle and might be related to the enlargement of muscle fiber diameter. MyoG, an important factor in regulating skeletal muscle development, was minimally expressed in the longissimus dorsi muscle and extensor digitorum longus, and highly expressed in the gastrocnemius and soleus muscles; it might inhibit the enlargement of muscle fiber diameter after birth. YAP1 expression was significantly (P < 0.05) or extremely significantly (P < 0.01) and positively correlated with MSTN and MyoG at 2 days old, 2 and 6 months old. YAP1 expression was related to muscle fiber development after birth and might be a candidate gene for the regulation of muscle growth.
Asunto(s)
Regulación del Desarrollo de la Expresión Génica , Miogenina/genética , Miostatina/genética , Ovinos/genética , Animales , Animales Recién Nacidos , Músculo Esquelético/crecimiento & desarrollo , Músculo Esquelético/metabolismo , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Ovinos/crecimiento & desarrollo , Factores de TiempoRESUMEN
The aim of the present study was to detect delta-like 1 ho-molog (DLK1) and insulin-like growth factor-I (IGF-I) gene expression in the longissimus dorsi of Hu sheep at different growth stages and study the association between these genes and meat quality. The diameter and density of muscle fibers and tenderness of the longissimus dorsi were measured. Growth stage, but not sex, significantly affected DLK1 and IGF-I expression. DLK1 and IGF-I expression in the sheep longissimus dorsi gradually increased with growth, but also decreased during some periods. These results suggest that different growth stages significantly affect DLK1 and IGF-I gene expression in sheep muscle tissue. The ex-pression of DLK1 and IGF-I genes were positively and significantly (P < 0.01) correlated with muscle fiber diameter and muscle fiber shear stress, and negatively and significantly (P < 0.01) correlated with muscle fiber density. Muscle fiber diameter was positively and significantly (P < 0.01) correlated with muscle fiber shear stress, and negatively and significantly (P < 0.01) correlated with muscle fiber density. In addition, DLK-1 expression was significantly (P < 0.01) and positively correlated with IGF-I expression.
Asunto(s)
Factor I del Crecimiento Similar a la Insulina/biosíntesis , Péptidos y Proteínas de Señalización Intercelular/biosíntesis , Carne , Proteínas de la Membrana/biosíntesis , Fibras Musculares de Contracción Lenta/metabolismo , Oveja Doméstica/genética , Animales , Expresión Génica , Factor I del Crecimiento Similar a la Insulina/genética , Músculo Esquelético/metabolismo , Oveja Doméstica/crecimiento & desarrolloRESUMEN
In the present study, real time-polymerase chain reaction was applied to analyze the expression of IGF-I and MyoG genes in Hu sheep longissimus dorsi at different growth stages and their association with meat traits. Expression of the IGF-I gene in Hu sheep differed significantly between males and females at the two day-old (0.01 < P < 0.05), one-month old (0.01 < P < 0.05), and three month-old (P < 0.01) stages. IGF-I gene expression in male longissimus muscles was higher than that of females at all growth stages, except for the three month-old stage. There was no significant difference (P > 0.05) between males and females at any growth stage in expression of the MyoG gene. MyoG gene expression in male longissimus muscles tended to be higher than that of females at all growth stages, except for the six month-old stage. IGF-I gene expression was significantly and positively correlated with live weight (P < 0.01) and carcass weight (0.01< P < 0.05), and was non-significantly positively correlated with net meat weight (P > 0.05). In contrast, MyoG gene expression was non-significantly and positively correlated with live weight, carcass, and net meat weight (P > 0.05). Carcass traits showed highly significant positive correlations (P < 0.01). Furthermore, expressions of IGF-I and MyoG genes showed highly significant positive correlations (P < 0.01). We conclude that the expressions of IGF-I and MyoG genes are significantly and positively correlated with early muscle traits of Hu sheep.
Asunto(s)
Factor I del Crecimiento Similar a la Insulina/biosíntesis , Carne , Miogenina/biosíntesis , Oveja Doméstica/genética , Animales , Peso Corporal/genética , Regulación del Desarrollo de la Expresión Génica , Factor I del Crecimiento Similar a la Insulina/genética , Músculo Esquelético/crecimiento & desarrollo , Miogenina/genética , FenotipoRESUMEN
Recent evidence suggests that genetic variations in the insulin-like growth factor (IGF)-IGF receptor (IGFR)-IGF binding proteins (IGFBP) axis may impact an individual's susceptibility to lung and esophageal cancer, but individually published results are inconclusive. Our meta-analysis aimed at providing a more precise estimation of these associations. An extensive literature search was conducted for appropriate articles published before May 15th, 2013. This meta-analysis was performed using the STATA 12.0 software. The crude odds ratios (ORs) with 95% confidence intervals (CIs) were calculated for each study and then pooled using a random effect model. Twelve case-control studies were included with a total of 2686 lung cancer patients, 771 esophageal cancer patients, and 5918 healthy controls. Our meta-analysis indicated that genetic variations in the IGF-IGFR-IGFBP axis may be associated with increased risk of lung and esophageal cancer, especially among Asian populations. Further subgroup analysis by gene type indicated that common polymorphisms in the IGF1/2, IGF-1R, and IGFBP-3/5 genes may be the main determinants for lung cancer risk, while IGF-1, IGF-1R, and IGFBP-1 genetic polymorphisms may increase the risk of esophageal cancer. The current meta-analysis suggests that genetic variations in the IGF-IGFR-IGFBP axis confer susceptibility to lung and esophageal cancer, especially among Asian populations. Common polymorphisms in the IGF-IGFR-IGFBP axis may serve as useful biomarkers for predicting the risk of lung and esophageal cancer.
Asunto(s)
Neoplasias Esofágicas/genética , Predisposición Genética a la Enfermedad , Variación Genética , Proteínas de Unión a Factor de Crecimiento Similar a la Insulina/genética , Neoplasias Pulmonares/genética , Receptores de Somatomedina/genética , Somatomedinas/genética , Estudios de Casos y Controles , Estudios de Asociación Genética , Humanos , Oportunidad Relativa , Sesgo de Publicación , RiesgoRESUMEN
Metallothionein (MT)-3 has cell growth inhibitory activity, and is the only currently known MT subtype with unique physiological functions. The expression levels of MT-1E, a subtype of MT-1, were positively correlated with the degree of esophageal cancer malignancy. The present study aimed to investigate the effects of MT-3 and MT-1E gene transfection on the proliferation, cell cycle, and apoptosis of esophageal cancer cells. The cationic liposome method was used to transfect the esophageal cancer strains Eca-109 and TE13. Reverse transcription-polymerase chain reaction was used to detect target gene expression, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide reduction was applied to detect cell proliferation, and flow cytometry was used for cell cycle and apoptosis detection. Esophageal cancer cells with MT-3 and MT-1E gene transfection showed high expression of the foreign target gene and mRNA. Cells with MT-3 gene transfection showed markedly inhibited proliferation (P < 0.05), a significantly higher proportion of cells in the G0/G1 phase (P < 0.05), a significantly lower proportion of cells in the S phase (P < 0.05), and a significantly increased apoptosis rate (P < 0.05). Cells with MT-1E gene transfection did not show significant changes in proliferation, cell cycle, or apoptosis rate (P > 0.05). Therefore, the upregulation of MT-3 gene expression can inhibit esophageal cancer cell proliferation and induce apoptosis, which may be achieved by blocking the tumor cell growth cycle, whereas effects of the MT-1E gene on the proliferation of esophageal cancer cells were not evident.
Asunto(s)
Apoptosis , Proliferación Celular , Neoplasias Esofágicas/metabolismo , Metalotioneína/genética , Proteínas del Tejido Nervioso/genética , Puntos de Control del Ciclo Celular , Línea Celular Tumoral , Neoplasias Esofágicas/patología , Expresión Génica , Humanos , Metalotioneína/biosíntesis , Metalotioneína 3 , Proteínas del Tejido Nervioso/biosíntesis , TransfecciónRESUMEN
Intracranial aneurysm is a balloon or sac-like dilatation of blood vessels inside the brain. Despite their importance, the biological mechanisms of intracranial aneurysms are not totally understood. We used public genome-wide gene expression profile data to identify potential genes that are involved in intracranial aneurysm in order to construct a regulation network. Some of the transcription factors and target genes that we identified in this network had been identified as related to intracranial aneurysm in previous studies. We found additional transcription factors and target genes that are apparently related to intracranial aneurysm with this method. The confirmation of previously identified genes and transcription factors supports the usefulness of this transcriptome network analysis for the identification of candidate genes involved in intracranial aneurysm.