RESUMEN
OBJECTIVE: To study the correlation of characteristic spectra of Vinegar Corydalis Rhizoma decoction pieces, water decoction and formula granules by HPLC, and to investigate the transfer of the main chemical constituents between three different forms. METHODS: The analysis was carried out by a Phenomenex Gemini C18 column (250 mm x 4.6 mm,5 µm) with acetonitrile-1% acetic acid and ammonium acetate buffer solution (pH 6.0) as the mobile phase in a gradient elution mode. The detection wavelength was 280 nm with a flow rate of 0.8 mL /min. The column temperature was 30 degrees C. The characteristic spectra from 11 batches of Vinegar Corydalis Rhizoma decoction pieces, 11 batches of water decoction and 11 batches of formula granules were established respectively. RESULTS: Ten peaks in the HPLC characteristic spectra from 11 batches of formula granules could be tracked in the water decoction, nine peaks in the HPLC characteristic spectra could be tracked in the decoction pieces. In the ten common peaks, four components such as protopine, palnatine chloride, berberine hydrochloride and tetrahydropalmatine were verified. CONCLUSION: The main chemical components of Vinegar Corydalis Rhizoma decoction pieces, water decoction and formula granules are basically the same, the common component contents have similar proportion.
Asunto(s)
Corydalis/química , Medicamentos Herbarios Chinos/química , Rizoma/química , Ácido Acético , Benzofenantridinas , Berberina , Alcaloides de Berberina , Cromatografía Líquida de Alta Presión , Temperatura , AguaRESUMEN
OBJECTIVE: To establish an HPLC fingerprint analysis method for Atractylodis Macrocephalae Rhizoma (warm dried). METHODS: Took methanol extract of Atractylodis Macrocephalae Rhizoma as sample by ultrasonic processing, HPLC analysis was carried out on Sinochrom ODS-BP (4.6 mm x 250 mm, 5 microm) chromatographic column with mobile phase of acetonitrile (A)-water (B), gradient elution with a flow at 1.0 mL/min, ultraviolet detection wavelength at 242 nm and column temperature at 30 degrees C. RESULTS: An HPLC fingerprint analysis method for Atractylodis Macrocephalae Rhizoma was developed. Eight common peaks were obtained and three peaks were identified. CONCLUSION: The method is accurate and credible which can be used for quality control of Atractylodis Macrocephalae Rhizoma (warm dried).