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1.
Asian J Androl ; 24(6): 601-606, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-35417989

RESUMEN

There is no well-established procedure for the management of small penis syndrome (SPS), especially when psychological interventions fail. This study aimed at systematically evaluating the physical and psychological benefits of penile augmentation (PA) using injectable hyaluronic acid (HA) gel. Thirty-eight patients receiving PA with HA gel from January 2017 to March 2020 were included and followed up for 1 year. Penile size, erectile function, and psychological burden measured by the Index of Male Genital Image (IMGI), Index of International Erectile Function (IIEF), and Beliefs about Penis Size (BPAS), respectively, were assessed at the beginning and at 1, 3, 6, and 12 months postinjection. The volume of HA gel injected was 21.5 ± 3.7 ml. Compared to baseline data, flaccid penile girth and length significantly increased by 3.41 ± 0.95 cm (P < 0.01) and 2.55 ± 0.55 cm (P < 0.01) at the 1st month postinjection, respectively. At the endpoint, despite attenuations, statistically significant improvements in flaccid penis size were still obtained, namely 2.44 ± 1.14 cm in girth (P < 0.01) and 1.65 ± 0.59 cm in length (P < 0.01). Similarly, erectile penile girth statistically increased by 1.32 ± 1.02 cm (P < 0.01) at the 1st month but were only 0.80 ± 0.54 cm bigger than baseline (P < 0.01) at the endpoint. At the 1st month, the average score of IMGI and the mean score of IIEF statistically increased by 46.2 ± 10.5 (P < 0.01) and 7.6 ± 6.2 (P < 0.01), respectively; the score of BAPS significantly decreased by 18.3 ± 4.5 (P < 0.01). These alterations remained steady during follow-up. Considering the significant penile size improvement, lasting psychological benefit, and low complication rate, PA with HA might serve as an appropriate alternative for patients with SPS.


Asunto(s)
Disfunción Eréctil , Ácido Hialurónico , Humanos , Masculino , Ácido Hialurónico/uso terapéutico , Disfunción Eréctil/etiología , Satisfacción del Paciente , Pene , Erección Peniana
2.
Sci Rep ; 7(1): 15322, 2017 11 10.
Artículo en Inglés | MEDLINE | ID: mdl-29127397

RESUMEN

MicroRNAs (miRNAs) regulate biological pathways by inhibiting gene expression. However, most current analytical methods fail to consider miRNAs, when inferring functional or pathway activities. In this study, we developed a model called sPAGM to infer subpathway activities by integrating gene and miRNA expressions. In this model, we reconstructed subpathway graphs by embedding miRNA components, and characterized subpathway activity (sPA) scores by simultaneously considering the expression levels of miRNAs and genes. The results showed that the sPA scores could distinguish different samples across tumor types, as well as samples between tumor and normal conditions. Moreover, the sPAGM model displayed more specificities than the entire pathway-based analyses. This model was applied to melanoma tumors to perform a prognosis analysis, which identified a robust 55-subpathway signature. By using The Cancer Genome Atlas and independently verified data sets, the subpathway-based signature significantly predicted the patients' prognoses, which were independent of clinical variables. In the prognostic performance comparison, the sPAGM model was superior to the gene-only and miRNA-only methods. Finally, we dissected the functional roles and interactions of components within the subpathway signature. Taken together, the sPAGM model provided a framework for inferring subpathway activities and identifying functional signatures for clinical applications.


Asunto(s)
Bases de Datos de Ácidos Nucleicos , Perfilación de la Expresión Génica , Regulación Neoplásica de la Expresión Génica , Melanoma/metabolismo , MicroARNs/biosíntesis , ARN Neoplásico/biosíntesis , Neoplasias Cutáneas/metabolismo , Femenino , Humanos , Masculino , Melanoma/genética , Melanoma/patología , MicroARNs/genética , ARN Neoplásico/genética , Neoplasias Cutáneas/genética , Neoplasias Cutáneas/patología
3.
Mol Med Rep ; 16(6): 8944-8952, 2017 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-28990109

RESUMEN

Cellular senescence is an irreversible growth arrest of cells that maintain their metabolic activities. Premature senescence can be induced by different stress factors and occurs in mouse embryonic fibroblasts (MEFs) derived from Zmpste24 metalloproteinase­deficient mice, a progeria mouse model of Hutchinson­Gilford Progeria Syndrome. Previous studies have shown that miR­342­5p, an intronic microRNA (miRNA/miR) reportedly involved in ageing associated diseases, is downregulated in Zmpste24­/­ MEFs. However, whether miR­342­5p is associated with the premature senescence phenotype of Zmpste24­/­ MEFs remains unclear. Thus, the present study investigated the effects of miR­342­5p on cellular senescence and cell proliferation in Zmpste24­/­ MEFs. The results showed that miR­342­5p overexpression ameliorated the cellular senescence phenotype to a certain extent, promoted cell proliferation and increased the G2+M cell cycle phase in Zmpste24­/­ MEFs. Nonetheless, it was difficult to observe the opposite cell phenotypes in wild­type (WT) MEFs transfected with the miR­342­5p inhibitor. Growth­arrest­specific 2 (GAS2) was identified as a target gene of miR­342­5p in Zmpste24­/­ MEFs. In addition, miR­342­5p was identified to be downregulated in WT MEFs during replicative senescence, while Gas2 was upregulated. Taken together, these findings suggest that downregulated miR­342­5p is involved in regulating cell proliferation and cell cycles in Zmpste24­/­ MEFs by suppressing GAS2 in vitro.


Asunto(s)
Fibroblastos/metabolismo , Regulación de la Expresión Génica , Proteínas de la Membrana/deficiencia , Metaloendopeptidasas/deficiencia , MicroARNs/genética , Proteínas de Microfilamentos/genética , Interferencia de ARN , Regiones no Traducidas 3' , Animales , Proliferación Celular , Supervivencia Celular/genética , Senescencia Celular , Puntos de Control de la Fase G2 del Ciclo Celular/genética , Ratones , Fenotipo
4.
Mutat Res ; 777: 69-78, 2015 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-25983189

RESUMEN

Zmpste24 is a metalloproteinase responsible for the posttranslational processing and cleavage of prelamin A into mature laminA. Zmpste24(-/-) mice display a range of progeroid phenotypes overlapping with mice expressing progerin, an altered version of lamin A associated with Hutchinson-Gilford progeria syndrome (HGPS). Increasing evidence has demonstrated that miRNAs contribute to the regulation of normal aging process, but their roles in progeroid disorders remain poorly understood. Here we report the miRNA transcriptomes of mouse embryonic fibroblasts (MEFs) established from wild type (WT) and Zmpste24(-/-) progeroid mice using a massively parallel sequencing technology. With data from 19.5 × 10(6) reads from WT MEFs and 16.5 × 10(6) reads from Zmpste24(-/-) MEFs, we discovered a total of 306 known miRNAs expressed in MEFs with a wide dynamic range of read counts ranging from 10 to over 1 million. A total of 8 miRNAs were found to be significantly down-regulated, with only 2 miRNAs upregulated, in Zmpste24(-/-) MEFs as compared to WT MEFs. Functional studies revealed that miR-365, a significantly down-regulated miRNA in Zmpste24(-/-) MEFs, modulates cellular growth phenotypes in MEFs. Overexpression of miR-365 in Zmpste24(-/-) MEFs increased cellular proliferation and decreased the percentage of SA-ß-gal-positive cells, while inhibition of miR-365 function led to an increase of SA-ß-gal-positive cells in WT MEFs. Furthermore, we identified Rasd1, a member of the Ras superfamily of small GTPases, as a functional target of miR-365. While expression of miR-365 suppressed Rasd1 3' UTR luciferase-reporter activity, this effect was lost with mutations in the putative 3' UTR target-site. Consistently, expression levels of miR-365 were found to inversely correlate with endogenous Rasd1 levels. These findings suggest that miR-365 is down-regulated in Zmpste24(-/-) MEFs and acts as a novel negative regulator of Rasd1. Our comprehensive miRNA data provide a resource to study gene regulatory networks in MEFs.


Asunto(s)
Proliferación Celular/genética , Fibroblastos/metabolismo , Perfilación de la Expresión Génica , Proteínas de la Membrana/metabolismo , Metaloendopeptidasas/metabolismo , MicroARNs/metabolismo , Secuencia de Aminoácidos , Animales , Senescencia Celular/genética , Regulación hacia Abajo , Fibroblastos/citología , Biblioteca de Genes , Secuenciación de Nucleótidos de Alto Rendimiento , Proteínas de la Membrana/genética , Metaloendopeptidasas/genética , Ratones , Ratones Noqueados , MicroARNs/genética , Datos de Secuencia Molecular , Proteínas ras/genética , Proteínas ras/metabolismo
5.
Bing Du Xue Bao ; 23(1): 22-7, 2007 Jan.
Artículo en Chino | MEDLINE | ID: mdl-17886716

RESUMEN

To explore the anti-HSV-1 effect of silencing gD gene expression by RNA interference, five 21-nucleotide duplex small interfering RNAs(siRNAs) targeting the HSV1 gD sequence were designed and the gD-EGFP fusion gene expression vector was constructed, then co-transfected into Vero cell, and screened the effective siRNA through analyzing the intensity of the EGFP fluorescence. Finally, the anti-HSV1 effect was confirmed by plaque reduction assay, real-time PCR and daughter virus titration of HSV1 infected Vero cells transfected with siRNAs. The study demonstrated that siRNAs could effectively and specifically inhibit gD gene expression in HSV1-infected cells, but only had a little effect on HSV1 infection, so taking gD as the target of siRNA against HSV1 needs further study.


Asunto(s)
Herpesvirus Humano 1/genética , Interferencia de ARN , Proteínas del Envoltorio Viral/genética , Animales , Chlorocebus aethiops , Vectores Genéticos/genética , Proteínas Fluorescentes Verdes/genética , Proteínas Fluorescentes Verdes/metabolismo , Humanos , Reacción en Cadena de la Polimerasa , ARN Interferente Pequeño/genética , Proteínas Recombinantes de Fusión/genética , Proteínas Recombinantes de Fusión/metabolismo , Transfección , Células Vero , Proteínas del Envoltorio Viral/metabolismo
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