RESUMEN
The number and the size of the Langerhans islets in the pancreata of most of investigated cases with diabetic foetopathy is increased (polynesia and macronesia). The B endocrine cells are immunoreactive for both insulin and IAPP, the reaction for IAPP being weaker in comparison with the controls. In one of the cases (the mother with long history of treated type 1 diabetes, who died during delivery) no presence of insulin-immunoreactive of PAF positive B cells is discovered. The reactivity for glucagon and somatostatin in the pancreata with diabetic foetopathy is found to be similar to the controls. In the vicinity of some islets lymphoid cell infiltrations are observed.
Asunto(s)
Diabetes Mellitus/congénito , Diabetes Mellitus/metabolismo , Enfermedades Fetales/metabolismo , Islotes Pancreáticos/metabolismo , Amiloide/metabolismo , Diabetes Mellitus/patología , Femenino , Enfermedades Fetales/patología , Glucagón/metabolismo , Humanos , Inmunohistoquímica , Recién Nacido , Insulina/metabolismo , Polipéptido Amiloide de los Islotes Pancreáticos , Islotes Pancreáticos/patología , Masculino , Somatostatina/metabolismoRESUMEN
The rabbit pancreatic structure at histological and ultrastructural level, in different intervals after ligature of pancreatic duct, was studied. Changes in the exo- and endocrine parenchyma were observed. Canalicular structures and conjunctive tissue replace the destroyed acinar cells. In some of the islets the B cells are degranulated or necrotic. Some islets contain infiltrations of lymphocytes, macrophages and granulocytes. Cavities formed by necrotic endocrine cells could appear. The A and B cells proliferation is observed in the new-formed clusters of endocrine cells and ductless. The mechanism of this process is similar to that of the islet formation during the embryonic development or in nesidioblastosis, but they are never transformed in the definitive islet of Langerhans.
Asunto(s)
Páncreas/patología , Conductos Pancreáticos/fisiología , Animales , Ligadura , Masculino , Microscopía Electrónica , Necrosis , Conejos , Factores de TiempoRESUMEN
The authors investigated histologically and immunohistochemically 60 cases with diabetes mellitus type 2. By means of the morphometric method of Weibel et al. (1969) they established diminished volume density of the insular tissue in total pancreas. The morphologic and morphometric data revealed evidence that diabetes mellitus most probably presented a heterogeneous group of pancreatic lesions manifested by disturbances of carbohydrate homeostasis.
Asunto(s)
Diabetes Mellitus Tipo 2/patología , Páncreas/patología , Diabetes Mellitus Tipo 2/metabolismo , Femenino , Histocitoquímica , Humanos , Masculino , Persona de Mediana Edad , Páncreas/metabolismoRESUMEN
Peri-insular lymphoid infiltrations were observed in nondiabetic C57BL/6J mice. The type of infiltrating cells was determined by direct immunoperoxidase technique. A larger part of lymphoid cells did not show reaction with a goat antimouse IgG Rh conjugate. Intensive hyperplastic reaction and the presence of many immunoglobulin producing cells were observed in the lymph nodes adjoining the pancreas. Probably the observed infiltrations in the pancreas of nondiabetic C57BL + 6J mice were composed mainly of T lymphocytes, belonging to T helper subpopulation.
Asunto(s)
Linfocitos/citología , Ratones Endogámicos C57BL/anatomía & histología , Páncreas/citología , Animales , Movimiento Celular , Heterocigoto , Técnicas para Inmunoenzimas , Inmunohistoquímica , Linfocitos/inmunología , Ratones , Ratones Endogámicos C57BL/inmunología , Páncreas/inmunologíaRESUMEN
9 surgically removed endocrine tumours of pancreas of female patients aged from 19 to 71 years were studied. The material was stained with hematoxylin and eosin, by Grimelius and Fontana--Masson; PAP method with polyclonal antibodies to insulin, glucagon and somatostatin was also used. Immunoreactivity was weaker in tumours with clinically pronounced hormonal activity as compared to the normal endocrine cells in the Langerhans islets. This is confirmed ultrastructurally: tumour cells contain comparatively low number of neuroendocrine granules. Functionally inactive tumours show the opposite immunohistochemical results. The multipotential properties of cells forming pancreatic endocrine tumours are discussed.
Asunto(s)
Adenoma de Células de los Islotes Pancreáticos/química , Adenoma de Células de los Islotes Pancreáticos/ultraestructura , Neoplasias Pancreáticas/química , Neoplasias Pancreáticas/ultraestructura , Adulto , Anciano , Femenino , Glucagón/análisis , Humanos , Inmunohistoquímica , Insulina/análisis , Persona de Mediana Edad , Somatostatina/análisisRESUMEN
Hypertrophy and hyperplasia of islet tissue, established by morphometry, were proved in the pancreas of rats, treated chronically for a period of 2 months (with urbason-50 mg/kg of body mass). B-cells were granulated. Structures with myelin-like form of membranes were observed in some of them by electron microscope. Mixed islet B-acinar cells were established as well. These data were possibly a morphological expression of stimulating action of urbason on B-cells of Langerhans islets.