RESUMEN
In a coded experiment on rabies virus antibody detection in the blood sera of humans immunized with rabies vaccines, coincidence of the results of indirect ELISA, neutralization test and radial hemolysis test (RHT) was observed in 83% and 90% of the cases. The correlation coefficient of antibody titres in ELISA and NT was r = +0.75, ELISA and RHT r = +0.81. Instances of discrepancy of the results were observed with sera of low titres, no more than 1:50 by neutralization test.
Asunto(s)
Anticuerpos Antivirales/análisis , Virus de la Rabia/inmunología , Animales , Estudios de Evaluación como Asunto , Técnica de Placa Hemolítica , Humanos , Técnicas para Inmunoenzimas , Ratones , Pruebas de Neutralización , Vacunas Antirrábicas/inmunología , Factores de TiempoRESUMEN
Electrophoregrams of the products of cleavage of DNA of 4 HSV-1 strains by restrictases Xbal, Hind III, and Sau 18/4 were compared. The sets of fragments obtained by the effect of Xbal restrictase on DNA of all 4 strains were found to be identical. At the same time, analysis of the products of DNA hydrolysis by Hind III and Sau 18/4 revealed significant interstrain differences.
Asunto(s)
Enzimas de Restricción del ADN/farmacología , ADN Viral/análisis , Simplexvirus/análisis , Secuencia de Aminoácidos , Animales , ADN Viral/clasificación , ADN Viral/aislamiento & purificación , Electroforesis en Gel de Agar , Peso Molecular , Cultivo de VirusRESUMEN
A comparatively simple method has been developed and tested for the detection and titration of antibody to Crimean hemorrhagic fever virus (CHF) by indirect immunofluorescence on slides with previously prepared acetone-inactivated virus in drops of cell suspension from infected BHK-21 and 6619 cell cultures. The antibody titers determined by the indirect immunofluorescence procedures were 4- and 8-fold higher than those determined by the complement-fixation test. This opens the possibility of using non-infectious antigens of cell suspensions for detection or titration by the FA procedure of antibody to CHF virus which may be of importance to diagnostic laboratories which have no conditions for work with live virus.