RESUMEN
Constitutional delay of growth and puberty (CDGP) is a variant of normal pubertal timing and progress. It is the most common form of delayed puberty in both genders. The genetic director of CDGP is ill-understood despite the positive family history result noted in those patients. The current study aimed at assessing the role of estrogen receptor 1 (ESR1) gene variant (rs827421) in Egyptian adolescents with CDGP. A cross-sectional study with follow-up part was carried out on 6760 children aged 4 to15 years. The study focused generally on children aged 13-15 years in order to evaluate the prevalence of delayed puberty in relation to all ages in general and to their peers in specific. Assessment of serum TSH, FSH, and LH was conducted on all participants, along with the measurement of serum-free testosterone for males and estradiol for females. Genotyping of ESR1 (rs827421) was done to all subjects through the use of TaqMan discrimination assay by real-time PCR. ESR1 (rs827421) GG genotype and G allele were significantly dominant among CDGP adolescents in comparison with controls (OR = 25.67 and 6.90). As regards follow-up of testicular size, AA genotype was significantly associated with increased size in the right and left testis compared to other genotypes (P = 0.021 and 0.006, respectively). Moreover, AA genotype showed significantly higher Tanner stage in both males and females in comparison with other genotypes. Serum estradiol level was significantly higher in AA genotype group than other genotypes groups. ESR1 gene polymorphism can be considered a potential genetic marker for CDGP in both sexes in a sample of Egyptian adolescents.
Asunto(s)
Pubertad , Adolescente , Niño , Estudios Transversales , Egipto , Estradiol , Femenino , Marcadores Genéticos , Humanos , Masculino , Polimorfismo Genético , Pubertad/genética , Testosterona/sangreRESUMEN
COVID-19 is a public Health Emergency of International Concern. The aim of this work was to assess the level of Knowledge, Attitude and Practice (KAP) among Egyptians toward COVID-19. A cross-sectional study was conducted from March 1st to April 1st, on 3712 participants of different ages and sex. An author designed KAP questionnaire toward COVID-19 administered online and personally was delivered. Satisfactory knowledge, positive attitude and good practice were reported among 70.2%, 75.9% and 49.2% of the participants respectively. Middle-aged participants reported high knowledge and attitude levels with poor practice level (p < 0.001). Females reported high knowledge and practice levels and low attitude (p < 0.001 and p = 0.041 respectively). Despite reporting high knowledge and attitude among urban residents (p < 0.001), practice level was high among rural residents (p = 0.001). Post-graduate education reported the highest levels of KAP (p < 0.001). Rural residents, working and non-enough income participants reported lower level of practice (p < 0.001). Logistic regression was carried out. It was found that unsatisfactory knowledge was associated with low education [Odds Ratio (OR) = 1.97, 95% CI: 1.51-2.56], and of rural residency (OR = 1.2, 95% CI: 1.05-1.41). Negative attitude was associated with not working (OR = 1.94, 95% CI: 1.61-2.35) and not enough income (OR = 1.29, 95%CI: 1.10-1.51 respectively). Poor practice is associated with young age (OR = 2.41, 95% CI: 1.94-2.98) and low education (OR = 1.19, 95% CI: 1.03-1.37) and not working (OR = 4.95, 95% CI: 4.07-6.02). Satisfactory knowledge, positive attitude and poor practice were found among the participants. A good knowledge and lower practice level were found among middle-aged, working participants, and participants with insufficient income. The demographic characters associated with KAP could be the cornerstone in directing policy-makers to target the health education campaigns to the suitable target groups.