RESUMEN
AIM: To select maximally HLA compatible donor for hematological patients who need transplantation of bone marrow from non-relative donor. MATERIALS AND METHODS: 75 patients with hematological malignancy were observed. All of them have indications to non-relative transplantation of the bone marrow. Methods of polymerase chain reaction with sequence-specific primers and classic microlymphocytotoxic test were used. RESULTS: Typing of HLA antigens of class I and alleles of class II loci enabled search for non-relative donor for transplantation of bone marrow in accordance with the requirements of the European Federation of Immunogenetics. Most of the patients (86.6%) had at least one potential HLA-A, -B, -DR compatible donor. Half of the patients had potential donors typed at the allele level by class II loci. This diminishes time of HLA compatible donor selection. CONCLUSION: DNA typing enables the search for the non-relative donors meeting modern requirements. This allowed 5 non-relative bone marrow transplantations.
Asunto(s)
Biomarcadores de Tumor/sangre , Antígenos HLA/sangre , Neoplasias Hematológicas/terapia , Trasplante de Células Madre Hematopoyéticas , Donantes de Tejidos , Electroforesis en Gel de Agar , Femenino , Antígenos HLA-DR/sangre , Antígenos HLA-DR/genética , Cadenas HLA-DRB1 , Trasplante de Células Madre Hematopoyéticas/efectos adversos , Prueba de Histocompatibilidad , Humanos , Masculino , Reacción en Cadena de la Polimerasa , Sistema de Registros , Trasplante HomólogoRESUMEN
AIM: To establish for hemoblastoses general and individual associations between HLA genes and predisposition or resistance to malignant transformation of hemopoiesis. MATERIAL AND METHODS: Antigens of class I and class II genes were determined by microlymphocytotoxic test and DNA typing (PCR-SSP) in 30 patients with acute myeloblastic leukemia (AML), 47 patients with acute lymphoblastic leukemia (ALL), 52 patients with chronic myeloblastic leukemia (CML), 58 patients with lymphogranulomatosis (LGM) and 224 donors. RESULTS: HLA specificities were elucidated frequency of which was high in all the above nosological entities: Cw7 (RR 2.5 to 5.22), DRB1*11 (RR 2.3 to 4.88). Frequency of antigen B5 was high in three diseases (RR 2.14-2.6). A number of specificities have distribution deviations typical for only one or two diseases (A19--AML and CML, B27--ALL, DRB1*08--AML). The HLA-gene was detected the frequency of which was low in three diseases--DRB1*07 (RR 0.39 to 0.11). Individual preventive HLA genes for AML, ALL, LGM were A2, B8; A1, B8, B40; DRB1*01, respectively. CONCLUSION: Cw7, DRB1*11, B5 are HLA-specificities marking hemopoiesis predisposition to malignant transformation in general. Development into a certain nosological entity depends on combination of general HLA-genes with markers of individual hemoblastosis. DRB1*07 is a general preventive gene. Preventive HLA genes can be also individual for the above hematological diseases.
Asunto(s)
Biomarcadores de Tumor/sangre , Marcadores Genéticos , Antígenos HLA/genética , Enfermedad de Hodgkin/genética , Leucemia Mieloide/genética , Leucemia-Linfoma Linfoblástico de Células Precursoras/genética , Enfermedad Aguda , Predisposición Genética a la Enfermedad , Antígenos HLA/sangre , Antígenos de Histocompatibilidad Clase I/sangre , Antígenos de Histocompatibilidad Clase I/genética , Antígenos de Histocompatibilidad Clase II/sangre , Antígenos de Histocompatibilidad Clase II/genética , Enfermedad de Hodgkin/inmunología , Humanos , Leucemia Mielógena Crónica BCR-ABL Positiva/genética , Leucemia Mielógena Crónica BCR-ABL Positiva/inmunología , Leucemia Mieloide/inmunología , Leucemia-Linfoma Linfoblástico de Células Precursoras/inmunologíaRESUMEN
AIM: To determine genetic factors of predisposition marked HLA with reference to serological and molecular characteristics. MATERIALS AND METHODS: Four groups of patients were included in the study: 51 patients with lymphogranulomatosis (LGM), 33 healthy relatives of these patients, 37 patients with chronic myeloid leukemia (CML), 24 healthy relatives of these CML patients. 224 donors served control. HLA-antigens were identified with the lymphocytoxic test and PSR-MSSR. Results of typing of class II antigens were taken into consideration in coincidence of serological and DNA typing. The significance of the differences was estimated according to the chi-square criterion. RESULTS: Differences in frequency of distribution of HLA-antigens (subloci A and B) were not found. Antigen CW7 was present in 70, DR5 in 60, DR6 in 50% of LGM patients. This frequency was much higher than in control groups. Carriers of CW7 are at 7 times higher risk to develop LGM. Among LGM patients the number of homozygous individuals is higher than in healthy ones (50 and 15.6%) while the number of individuals with a complete set of HLA-A.B antigens is significantly less. CONCLUSION: Genetic predisposition to LGM is predetermined by HLA antigens CW7, DR5, DR6. Genes HLA-DR1 and HLA-DR7 protect carriers from factors provoking LGM. Common HLA genes in the parents predispose their children to LGM. Insufficiency of the phenotype is a factor predisposing to LGM.
Asunto(s)
Enfermedad de Hodgkin/genética , Distribución de Chi-Cuadrado , Susceptibilidad a Enfermedades , Marcadores Genéticos/genética , Antígenos HLA/sangre , Antígenos HLA/genética , Enfermedad de Hodgkin/inmunología , Homocigoto , Humanos , Inmunogenética , Fenotipo , Factores de RiesgoRESUMEN
Immunoreactivity (proliferative capacity) of normal donor peripheral blood mononuclears expressing HLA-DR antigens in MLC test was studied. HLA-DR cellular phenotype was found to influence the intensity of proliferative response. HLA-DR highly reactive (HLA-DR5, HLA-DR7, HLA-DR4) and low-reactive (HLA-DR1, HLA-DR2, HLA-DR6) determinants were distinguished.
Asunto(s)
Reacciones Antígeno-Anticuerpo , Antígenos HLA-DR/sangre , Linfocitos/inmunología , Adulto , Células Cultivadas , Estudios de Evaluación como Asunto , Humanos , Persona de Mediana EdadRESUMEN
Upon the analyses of outcomes of 69 primary allogenic kidney transplantations it was noted that cyclosporin A immunosuppressive potential grew in transplantation of the kidney more compatible by HLA-DR antigens. It follows that it would be valid to perform tissue selection in the above use of the drug cyclosporin A.
Asunto(s)
Ciclosporina/uso terapéutico , Antígenos HLA-DR/sangre , Histocompatibilidad/inmunología , Trasplante de Riñón/inmunología , Donantes de Tejidos , Supervivencia de Injerto/efectos de los fármacos , Supervivencia de Injerto/inmunología , Humanos , Terapia de Inmunosupresión/métodos , Trasplante Homólogo , Resultado del TratamientoRESUMEN
The paper presents a review of the activities of the National Register of Typed Donors in connection with 1.5-year activity of the World Donor of Bone Marrow Association. The search for donors was performed for all European and American countries using myelotransformation from unrelated donor. Basing on I class HLA-antigen phenotyping frequency, split typing and DR-antigens, conclusions are drawn as to the condition of tissue typing by the register as a whole and for separate regions of the country. The composition and contamination with nonmonospecific sera of the National Panel of Typing Sera have been reviewed in comparison with reference panel one lambda.
Asunto(s)
Sistema de Registros , Donantes de Tejidos/clasificación , Transfusión Sanguínea , Trasplante de Médula Ósea , Antígenos HLA/análisis , Prueba de Histocompatibilidad/estadística & datos numéricos , Humanos , Cooperación Internacional , Sistema de Registros/estadística & datos numéricos , Federación de Rusia , Donantes de Tejidos/estadística & datos numéricos , U.R.S.S.RESUMEN
The incidence rate of HLA class I and II antigens has been studied in 168 and 82 patients with diffuse toxic goiter, respectively. Positive association is found for HLA antigens A2, A3, A28, B8, B15, B17, Cw4, DR5; negative one with antigens A24, Bw4, Bw6, Cw1, Cw2, Cw3, Cw5, DR9. Prevalence of antigens B15, B17, Cw4, DR5 is associated with thyrotoxicosis severity, A2 with recurrent toxic goiter. Antigen B8 plays the role of an immunogenetic marker of endocrine ophthalmopathy.
Asunto(s)
Enfermedades Autoinmunes/inmunología , Enfermedad de Graves/inmunología , Antígenos de Histocompatibilidad Clase II/sangre , Antígenos de Histocompatibilidad Clase I/sangre , Adulto , Enfermedades Autoinmunes/epidemiología , Biomarcadores/sangre , Donantes de Sangre , Distribución de Chi-Cuadrado , Enfermedad Crónica , Femenino , Enfermedad de Graves/epidemiología , Haplotipos , Humanos , Incidencia , Masculino , Moscú/epidemiología , Tirotoxicosis/epidemiología , Tirotoxicosis/inmunologíaAsunto(s)
Síndrome de Cushing/genética , Corteza Suprarrenal/fisiopatología , Animales , Enfermedades Autoinmunes/clasificación , Enfermedades Autoinmunes/diagnóstico , Enfermedades Autoinmunes/etiología , Enfermedades Autoinmunes/genética , Síndrome de Cushing/clasificación , Síndrome de Cushing/diagnóstico , Síndrome de Cushing/etiología , Antígenos HLA/genética , Antígenos HLA/inmunología , Humanos , Hipófisis/fisiopatologíaRESUMEN
Ninety-seven patients suffering from Itsenko [correction of Icenko]-Cushing disease were examined. Analysis was made first of all of the clinical and laboratory signs pointing to the presence of other endocrine diseases to distinguish two groups of patients. The first group included subjects who did not manifest any concomitant diseases. The number of men and women was the same. The second group was made up of patients with associated Itsenko [correction of Icenko]-Cushing disease and other endocrine diseases. In this particular group, women were predominant. The first group patients demonstrated the predominance of the DR7 incidence, the second group that of the DR5. The method of estimating HLA associations identified is proposed. It allows distinguishing specific and nonspecific associations. In Itsenko [correction of Icenko]-Cushing disease, DR7 association is specific, whereas B8 and DR5 associations are unspecific. A specific polyendocrine autoimmune syndrome associated with Itsenko [correction of Icenko]-Cushing disease was revealed. A concept of the pathogenesis of Itsenko [correction of Icenko]-Cushing disease is advanced.
Asunto(s)
Síndrome de Cushing/inmunología , Enfermedades del Sistema Endocrino/inmunología , Adulto , Enfermedades Autoinmunes/genética , Enfermedades Autoinmunes/inmunología , Enfermedad Crónica , Síndrome de Cushing/genética , Enfermedades del Sistema Endocrino/genética , Femenino , Antígenos HLA/sangre , Antígenos HLA/genética , Humanos , Inmunogenética , Síndromes de Inmunodeficiencia/genética , Síndromes de Inmunodeficiencia/inmunología , MasculinoRESUMEN
Two hundred and thirty Russian Moscovities (108 healthy individuals and 122 cadaver kidney donors) were typed for antigens HLA-DR1-DRw10, HLA-DRw52-DRw53, and HLA-DQw1 and DQw3. HLA-DR1, 2, 3, 4, 5 and 7 were investigated in all individuals, the other having been only studied in portions of the material. The HLA class 2 antigens' polymorphism in Russian population has been shown to have features common to all Caucasians. Most widely distributed antigen is DR2 (phenotype frequency is 36%). Antigens DRw8, DR9 and DRw10 are rare. DR allele distribution demonstrated perfect fits to Hardy--Weinberg expectations. Antigens DR5 and DR4 are considered most difficult to define at early stage of the study.
Asunto(s)
Antígenos de Histocompatibilidad Clase II/genética , Polimorfismo Genético/genética , Alelos , Humanos , Moscú/etnología , Pruebas SerológicasRESUMEN
Incidence of class I and II HLA antigens was studied in 69 patients suffering from Icenko--Cushing syndrome. Positive association was reported for antigens DRw53, DQw3, DR4, DR5, negative for antigen DR2. Antigen combinations DR5, DR7 and DR4, DR5 proved more prevalent. The risk to develop the disease rose 2-3-fold in case of HLA-DR5 phenotype occurrence with antigen DR4 or DR7. Association with DRw53 and DQw3 antigens showing wide specificity seemed most significant.
Asunto(s)
Síndrome de Cushing/genética , Antígenos de Histocompatibilidad Clase II/genética , Antígenos de Histocompatibilidad Clase I/genética , Adulto , Síndrome de Cushing/etiología , Síndrome de Cushing/inmunología , Epítopos/análisis , Epítopos/genética , Epítopos/inmunología , Femenino , Antígenos de Histocompatibilidad Clase I/análisis , Antígenos de Histocompatibilidad Clase I/inmunología , Antígenos de Histocompatibilidad Clase II/análisis , Antígenos de Histocompatibilidad Clase II/inmunología , Humanos , Masculino , Persona de Mediana Edad , Fenotipo , Factores de RiesgoRESUMEN
The changes in tactic approaches may be recorded in clinical transplantation of the bone marrow (TBM). The countries of Europe and America realize TBM from the HLA-compatible unrelated donor. The first stage in such TBM is the establishment of the All-Union Register of typified donors which includes about 8,000 persons and is the member of the World Donor International Association. The authors describe the scientific and organizational principles of the work in the international regime; provide the ethnic and genogeographic characteristics of the All-Union Register. Give brief data on the activity of the All-Union Register during April 15 -- December 1, 1990.
Asunto(s)
Trasplante de Médula Ósea/inmunología , Prueba de Histocompatibilidad , Sistema de Registros , Donantes de Tejidos , Obtención de Tejidos y Órganos/organización & administración , Genética de Población , Antígenos HLA/sangre , Humanos , Trasplante Homólogo , U.R.S.S.RESUMEN
A programme including prophylactic, diagnostic aspects and reasons for the therapeutic tactics has been proposed for narrowing leukemia consequences related to the catastrophe at Chernobyl NPS. The state of the All-Union Register of typed donors and All-Union standard of typing sera has been considered. It is necessary to make reserves of blood containing no antibodies to cytomegalovirus (CMV-negative blood).
Asunto(s)
Accidentes , Transfusión Sanguínea , Trasplante de Médula Ósea/inmunología , Antígenos HLA/efectos de la radiación , Síndromes de Inmunodeficiencia/prevención & control , Leucemia Inducida por Radiación/prevención & control , Reactores Nucleares , Centrales Eléctricas , Ceniza Radiactiva/efectos adversos , Antígenos HLA/inmunología , Humanos , Síndromes de Inmunodeficiencia/etiología , Síndromes de Inmunodeficiencia/terapia , Leucemia Inducida por Radiación/inmunología , Leucemia Inducida por Radiación/terapia , UcraniaRESUMEN
Serological polymorphism of HLA 1 class genes and antigens (A, B, C) was studied in native West-Siberian population of Khants. Genes HLA-A2, A23, A24, B7, B35, B49, Cw4 and Cw7 appeared to be most frequent in this population. HLA-A23/B49 was the most wide-spread haplotype with strong linkage disequilibrium. The main features of the HLA polymorphism indicate profound mongoloid roots of Khants, whereas strong linkage disequilibrium of some HLA alleles confirms the idea of the origin of Khants as a result of ancient mixing of mongoloids and caucasoids.
Asunto(s)
Pueblo Asiatico/genética , Etnicidad , Antígenos HLA/genética , Polimorfismo Genético , Frecuencia de los Genes , Haplotipos , Humanos , SiberiaRESUMEN
Three functional models relating the integral index of transplantation outcome to a combination of immunogenetic status determinants in the members of the donor-recipient pair were constructed using statusometric techniques. These models for the first time make it possible to perform a quantitative assessment of the contribution of each of the 24 A and B locus HLA antigens and four blood groups of the recipient, of the role of coincidence and discrepancy among the determinants making up the combination, and to automate selection of the most suitable recipient for every donor kidney from waiting lists.