RESUMEN
BACKGROUND: Peanut storage proteins (Ara h 1, Ara h 2, and Ara h 3) have been described as the major peanut allergens in children, although not all peanut-sensitized individuals have clinical reactivity after exposure. OBJECTIVES: We studied the sensitization profile of peanut-allergic and peanut-tolerant children in a pediatric cohort. METHODS: The clinical features and sensitization profile to the peanut storage proteins Ara h 9 and Pru p 3 were compared between peanut-allergic and peanut-tolerant children using component-resolved diagnostics. RESULTS: Thirty-three peanut-sensitized children were included: 22 allergic and 11 tolerant patients. Seventy-two percent of the peanut-allergic children were sensitized to at least one peanut storage protein. The rates of sensitization to Ara h 1, Ara h 2, and Ara h 3 were 63.6, 68.1, and 68.1%, respectively, among the peanut-allergic children and 27.2, 18.1, and 45.4% among the peanut-tolerant children. IgE from the sera of 18% of the peanut-allergic patients recognized Ara h 9, whereas no sensitization to Ara h 9 was detected in the peanut-tolerant children. A total of 59% of the peanut-allergic and 27% of the peanut-tolerant children were sensitized to Pru p 3. Sensitization to Ara h 1 and Ara h 2 was more frequent among the peanut-allergic children (p < 0.05). Although the levels of specific IgE against peanut storage proteins were higher in peanut allergy, there were not statistically significantly different from the levels in peanut tolerance, probably due to the small number of patients included. CONCLUSIONS: In our population, the peanut-allergic children were mainly sensitized to peanut storage proteins, and Ara h 2 sensitization allows a more accurate diagnosis of clinical reactivity to peanuts. More than half of the peanut-allergic patients were sensitized to peach Pru p 3, and 50% of them had fruit allergy at the time of the study.
Asunto(s)
Tolerancia Inmunológica , Hipersensibilidad al Cacahuete/inmunología , Albuminas 2S de Plantas/inmunología , Adolescente , Antígenos de Plantas/inmunología , Niño , Preescolar , Femenino , Glicoproteínas/inmunología , Humanos , Inmunoglobulina E/sangre , Masculino , Proteínas de Plantas/inmunologíaRESUMEN
UNLABELLED: Contact with pine processionary caterpillar (Thaumetopoea pityocampa) induces dermatitis usually located in exposed areas through a toxic-irritative mechanism. Over the last few years an immediate hypersensitivity mechanism have mainly been demonstrated in adult patients. However, there are few studies carried out in children. OBJECTIVE: To evaluate a group of 16 children who experienced allergic reactions after exposure to pine processionary caterpillar. PATIENTS AND METHODS: All patients underwent allergy testing through skin prick test. Serum specific IgE determination was performed by EAST technique. The molecular mass of the IgE -binding bands was studied by SDS-PAGE Immunoblotting. RESULTS: Skin prick test with the caterpillar extract was positive in all patients. Specific IgE was positive (higher than 0.35 kU/L) in 15 patients' sera. Western blotting showed several IgE-binding bands with molecular mass values ranging from 17.5 to 168 kDa. Electrophoretic mobility of some of the relevant allergens was related to the conditions of sample preparation (reduced or non-reduced). CONCLUSIONS: The results of this study demonstrate the existence of an allergic IgE-mediated mechanism caused by pine processionary caterpillar proteins. Airborne urticating hairs of this animal should be considered as seasonal inhalant allergen, which is able to induce allergic pathologies in children who frequent pine areas.
Asunto(s)
Asma/etiología , Dermatitis Alérgica por Contacto/etiología , Mariposas Nocturnas/inmunología , Urticaria/etiología , Adolescente , Anafilaxia/etiología , Animales , Especificidad de Anticuerpos , Western Blotting , Niño , Conjuntivitis Alérgica/etiología , Femenino , Liberación de Histamina , Humanos , Inmunoglobulina E/sangre , Inmunoglobulina E/inmunología , Larva , Masculino , Mariposas Nocturnas/crecimiento & desarrollo , Prurito/etiología , Rinitis Alérgica Estacional/etiología , Pruebas Cutáneas , Extractos de Tejidos/efectos adversos , Extractos de Tejidos/inmunología , Extractos de Tejidos/aislamiento & purificaciónRESUMEN
Contact with pine processionary caterpillar (Thaumetopoea pityocampa) induces dermatitis usually located in exposed areas through a toxic-irritative mechanism. Over the last few years an immediate hypersensitivity mechanism have mainly been demonstrated in adult patients. However, there are few studies carried out in children. Objective: To evaluate a group of 16 children who experienced allergic reactions after exposure to pine processionary caterpillar. Patients and methods: All patients underwent allergy testing through skin prick test. Serum specific IgE determination was performed by EAST technique. The molecular mass of the IgE binding bands was studied by SDS-PAGE Immunoblotting. Results: Skin prick test with the caterpillar extract was positive in all patients. Specific IgE was positive (higher than 0.35 kU/L) in 15 patients' sera. Western blotting showed several IgE-binding bands with molecular mass values ranging from 17.5 to 168 kDa. Electrophoretic mobility of some of the relevant allergens was related to the conditions of sample preparation (reduced or non-reduced). Conclusions: The results of this study demonstrate the existence of an allergic IgE-mediated mechanism caused by pine processionary caterpillar proteins. Airborne urticating hairs of this animal should be considered as seasonal inhalant allergen, which is able to induce allergic pathologies in children who frequent pine areas
El contacto con la procesionaria del pino (Thaumetopoea pityocampa) produce diversos cuadros cutáneos, localizados, principalmente, en zonas expuestas, mediante un mecanismo tóxixo-irritativo. En los últimos años se ha demostrado un mecanismo de hipersensibilidad inmediata en pacientes generalmente adultos. Sin embargo, los estudios realizados en niños son escasos. Objetivo: Estudiar 16 casos de niños que sufrieron reacciones alérgicas tras exposición a procesionaria del pino. Material y métodos: En todos los pacientes se realizó estudio alergológico mediante pruebas cutáneas en prick y detección de IgE específica en el suero. Se estudió la masa molecular de las proteínas fijadoras de IgE específica mediante la técnica de SDS-PAGE Immunoblotting. Resultados: Las pruebas cutáneas con el extracto de procesionaria del pino fueron positivas en todos los pacientes. La detección de IgE específica fue positiva ( > 0,35 kU/L) en 15 pacientes. En el Immunoblotting se detectaron varias bandas fijadoras de IgE, con masas moleculares comprendidas entre 17.5 y 168 kDa. La movilidad electroforética de algunos de los alérgenos relevantes se modifica por la presencia de un agente reductor (β-mercaptoetanol). Conclusión: Los resultados de este estudio muestran un mecanismo alérgico de hipersensibilidad mediado por IgE en estas reacciones. Las espículas urticantes aerotransportadas de la procesionaria del pino deberían considerarse como potenciales neumoalérgenos estacionales, capaces de desencadenar patologías alérgicas en niños que frecuenten zonas próximas a pinares