RESUMEN
BOX, ERIC and REP--genomic fingerprints of 12 isolated and 10 typical pathogenic for rape bacterial strains Pseudomonas, Xanthomonas and Pectobacterium genera have been analyzed. The affinity of isolated strains with representatives of P. marginalis pv. marginalis, Pseudomonas fluorescens and Xanthomonas campestris pv. campestris species has been determined.
Asunto(s)
Brassica rapa/microbiología , ADN Bacteriano/genética , Pectobacterium/aislamiento & purificación , Enfermedades de las Plantas/microbiología , Pseudomonas/aislamiento & purificación , Xanthomonas/aislamiento & purificación , Electroforesis en Gel de Agar/métodos , Pectobacterium/genética , Reacción en Cadena de la Polimerasa/métodos , Pseudomonas/genética , Xanthomonas/genéticaRESUMEN
The fatty acid composition of cell lipids of 15 strains isolated from the affected plants of rape and five collection strains has been studied. According to the results of chemotaxonomic analysis it has been found that 9 isolated strains are similar to representatives of species Pseudomonas marginalis and Pseudomonas fluorescens, and 6 - to those of Xanthomonas campestris pv. campestris. The authors have established the efficiency of certain methods for the extraction of fatty acids used for the identification of bacteria pathogenic for rape which belong to the genera Pseudomonas and Xanthomonas.
Asunto(s)
Brassica rapa/microbiología , Ácidos Grasos/aislamiento & purificación , Enfermedades de las Plantas/microbiología , Pseudomonas fluorescens/química , Pseudomonas/química , Xanthomonas campestris/química , Cromatografía Liquida , Ácidos Grasos/química , Espectrometría de Masas , Metilación , Filogenia , Pseudomonas/clasificación , Pseudomonas/aislamiento & purificación , Pseudomonas fluorescens/clasificación , Pseudomonas fluorescens/aislamiento & purificación , Xanthomonas campestris/clasificación , Xanthomonas campestris/aislamiento & purificaciónRESUMEN
Bacterial destruction of the culture was described and its agents identified in the spring and winter rape crops. Typical symptoms are the following: browning of stem tissue and its mucilagization, chlorosis of leaves, yellowing and beginning of soft rot in the place of leaf stalks affixion to stems, loss of pigmentation (violet). Pathogenic properties of the collection strains and morphological, cultural, physiological, and biochemical properties of the agents of rape's bacterial diseases isolated by the authors have been investigated. It was found that all the isolates selected by the authors are highly or moderately aggressive towards different varieties of rape. According to the complex of phenotypic properties 44% of the total number of isolates selected by the authors are related to representatives of the genus Pseudomonas, 37% - to Xanthomonas and 19% - to Pectobacterium.
Asunto(s)
Brassica napus/microbiología , Pectobacterium/patogenicidad , Pseudomonas/patogenicidad , Xanthomonas/patogenicidad , Enfermedades de las Plantas/microbiología , Hojas de la Planta/microbiología , Tallos de la Planta/microbiologíaRESUMEN
The technique of fluorescence recovery after photobleaching was used to measure the lateral mobility of membrane integral proteins in reticulocyte plasma membranes which were treated to modify the 'fluid' lipid or immobilized protein fractions, hence increasing the relative prevalence of obstacles to protein lateral motion. This was achieved by either: (1) treating the plasma membranes with phospholipase A2 followed by extraction of the hydrolysis products using fatty-acid-free bovine serum albumin, resulting in a decrease in the membrane 'fluid' lipid portion; or (2) preincubating the plasma membranes with polylysines, resulting in plasma membrane protein aggregation and immobilization. As the prevalence of obstacles to lateral motion increased in plasma membranes through the treatments described above, the mobility of the membrane integral proteins diminished. Experimental results for the dependence of protein mobility on the prevalence of obstacles to lateral motion were compared to theoretical data in order to verify the applicability of the percolation theory to reticulocyte plasma membranes. The influence of a decrease in the 'fluid' lipid and an increase in the immobilized membrane protein fractions upon the hormone-stimulated adenylate cyclase activity has been studied as well. As the 'solid' lipid and immobilized membrane protein fractions decreased, both the hormone-stimulated adenylate cyclase activity and the fraction of beta-adrenergic receptors with high affinity to hormone diminished. It was shown that this correlation can be caused by a decrease in membrane fraction accessible to the movement of the interacting proteins of the adenylate cyclase complex. Hormonal stimulation of adenylate cyclase is discussed in terms of the percolation theory.