RESUMEN
The aim of this study was to determine the changes in the pancreas structure after acute and chronic exposure of organism to gravitational overloads (GO). The experiments were conducted in 36 outbred albino male rats, 12 rats formed the control group. GO effect was modeled using the centrifuge; the value of the overload thus created amounted to 4,0-6,0 gravitational units. Pancreas structure was studied using histological, electron microscopic and morphometric methods. It was found that after acute exposure to GO, mainly the reactive changes were demonstrated all the pancreatic structures studied: acinocytes, endocrinocytes, blood vessels and nervous apparatus. Chronic exposure to GO resulted in both reactive modifications, similar to those described after an acute exposure, and various compensatory-adaptive and destructive changes.
Asunto(s)
Hipergravedad/efectos adversos , Vaina de Mielina/ultraestructura , Páncreas/patología , Animales , Masculino , Microscopía Electrónica de Transmisión , Páncreas/irrigación sanguínea , Páncreas/ultraestructura , Conductos Pancreáticos/irrigación sanguínea , Conductos Pancreáticos/patología , RatasRESUMEN
AIM: To evaluate effectiveness of OSMO-adalat in combination of arterial hypertension (AH) with kinked precerebral arteries (KPA). MATERIAL AND METHODS: Before and after a 3-month course of OSMO-Adalat in a dose 30 and 60 mg, 28 patients with AH (degree 2-3) and KPA were examined for arterial pressure (AP), dyscirculatory encephalopathy, orthostatic stability, ischemic heart disease, transitory ischemic attacks, side effects of therapy. RESULTS: A complete normalization of AP was achieved in 9(32.1%) patients, in the rest patients the effect was partial. 24-h AP profile improved in all the cases. OSMO-Adalt relieved coronary insufficiency and dyscirculatory encephalopathy, improved tolerance of orthostatic loads. Syncopes, transitory ischemic attacks and strokes were not observed. Severe head ache was the cause of the treatment discontinuation in one patient. CONCLUSIONS: Therapy with OSMO-Adalat of patients with AH combination with KPA provides a good hypotensive effect and relieves symptoms of encephalopathy, myocardial ischemia and orthostatic insufficiency in low risk of side effects.
Asunto(s)
Antihipertensivos/uso terapéutico , Bloqueadores de los Canales de Calcio/uso terapéutico , Enfermedades de las Arterias Carótidas/etiología , Hipertensión/tratamiento farmacológico , Nifedipino/uso terapéutico , Anciano , Antihipertensivos/efectos adversos , Presión Sanguínea , Bloqueadores de los Canales de Calcio/efectos adversos , Enfermedades de las Arterias Carótidas/patología , Preparaciones de Acción Retardada , Mareo , Femenino , Humanos , Hipertensión/complicaciones , Hipertensión/fisiopatología , Ataque Isquémico Transitorio/etiología , Masculino , Persona de Mediana Edad , Isquemia Miocárdica/etiología , Nifedipino/administración & dosificación , Nifedipino/efectos adversos , SíncopeAsunto(s)
Hormona del Crecimiento/metabolismo , División Celular , Línea Celular , Hormona del Crecimiento/química , Hormona del Crecimiento/genética , Humanos , Mutagénesis Sitio-Dirigida , Estructura Terciaria de Proteína , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismoRESUMEN
In the present study alpha 1-adrenergic receptors have been investigated in liver parenchyma, obtained at the resection of prehepatic portal hypertension children without parenchymal affection (control group, n = 7) and the resection of children in parenchymal affection (group of cirrhosis, n = 8). It has been shown, that the binding of alpha 1-adrenergic antagonist 3H-prasozin (3H-PRZ) in liver parenchyma membranes of both control and cirrhosis groups is saturable and shows a high affinity. The Scatchard analysis of the binding data indicated that the binding site is characterized by Kd and Bmax of 0.6 +/- 0.12 nM, 92.8 +/- 8.0 fmol/mg, respectively, for the control group; and 1.5 +/- 0.4 nM, 254.1 +/- 28.4 fmol/mg, respectively, for the group of cirrhosis; (mean +/- SEM). It has been found that the number of binding sites of 3H-PRZ significantly increases in cirrhosis liver parenchyma in comparison with the control group. The results obtained suggest that alpha 1-adrenergic receptors play an important role in cirrhosis formation in children, showing liver parenchyma affection severity and its regenerative properties.
Asunto(s)
Cirrosis Hepática/metabolismo , Hígado/química , Receptores Adrenérgicos alfa/análisis , Adolescente , Biopsia , Niño , Preescolar , Humanos , Hipertensión Portal/metabolismo , Hipertensión Portal/patología , Hígado/patología , Cirrosis Hepática/etiología , Cirrosis Hepática/patología , Ensayo de Unión RadioliganteRESUMEN
In the present study adrenergic receptors have been investigated in liver parenchyma, obtained at the resection of extrahepatic portal hypertension children without parenchymal affection (control group, n-7) and the resection of children in parenchymal affection (group of chronic hepatitis children, n-6). It has been shown, that the binding of beta-adrenergic radioligand 3H-dihydroalprenolol (3H-DHA) in liver parenchyma membranes of both control and chronic hepatitis groups was saturable and showed high affinity. The Scatchard analysis of the binding data indicated that the binding site was characterized by Kd and Bmax of 1.2 +/- 0.5 nM, 261.2 +/- 50 fmol/mg, respectively, for the control group; and 0.9 +/- 0.15 nM, 68.5 +/- 18.8 fmol/mg, respectively, for the group of chronic hepatitis patients; (mean+SEM). The binding of alpha 1-adrenergic antagonist 3H-prazosin (3H-PRZ) in liver parenchyma was also saturable and showed high affinity. The binding site is characterized by Kd = 0.6 +/- 0.12 nM, Bmax = 92.8 +/- 8.0 fmol/mg, for the control group; and Kd = 0.8 +/- 0.15 nM, Bmax = 195.0 +/- 22.0 fmol/mg, for the group of chronic hepatitis. It has been found that the number of binding sites of 3H-DHA significantly decreased and the number of binding sites of 3H-PRZ did not change in chronic hepatitis liver parenchyma in comparison with the control group. The results obtained suggest the important role of beta-adrenergic receptors in the pathogenesis of chronic hepatitis and in liver regeneration in children.
Asunto(s)
Hepatitis/metabolismo , Hígado/química , Receptores Adrenérgicos/análisis , Adolescente , Sitios de Unión , Niño , Preescolar , Enfermedad Crónica , Dihidroalprenolol/metabolismo , Humanos , Prazosina/metabolismo , Ensayo de Unión Radioligante , Receptores Adrenérgicos/metabolismo , Receptores Adrenérgicos alfa/análisis , Receptores Adrenérgicos alfa/metabolismo , Receptores Adrenérgicos beta/análisis , Receptores Adrenérgicos beta/metabolismoRESUMEN
A clinico-psychological study covered 28 children aged 4-13 years with protracted hypomanic states (2-8 years) in 3-10-year-long schizophrenia. The authors investigated peculiarities of the development of cognitive activity (thought and perception). Comparison was made between groups of schizophrenics and control groups (the syndrome of motor disinhibition, schizoid psychopathy). The findings of a clinico-psychological examination have helped to elucidate differences in the development of cognitive activity in the above groups of children which may be used for the differential diagnosis and prognosis of the disease course.