RESUMEN
A virus, designated Sikhote-Alin, was isolated in 1970 from Ixodes persulcatus ticks collected from a wild boar in the Primorie region (U.S.S.R.) Sikhote-Alin virus showed no haemagglutinating activity and no antigenic relationships with arboviruses of 12 antigenic groups, 17 ungrouped tick-borne arboviruses, porcine enteroviruses and coxsackie A (types 1-18) viruses. An one-way antigenic relationship was demonstrated by complement fixation with cardioviruses (Mengo and Columbia-SK strains). The virus contains RNA, is resistant to lipid solvents, highly thermostable in the presence of 1 M MgCl2 and its size is over 20 nm but less than 25 nm. All these properties make it possible to consider it as a new member of the cardiovirus group (genus Enterovirus; Picornaviridae).
Asunto(s)
Grupos de Población Animal/parasitología , Animales Salvajes/parasitología , Picornaviridae/clasificación , Porcinos/parasitología , Garrapatas/microbiología , Animales , Femenino , Ratones , Picornaviridae/aislamiento & purificación , Picornaviridae/fisiología , U.R.S.S.RESUMEN
A virus, designated Razdan, was isolated from Dermacentor marginatus ticks in the Armenian S.S.R. in 1973. The complement fixation tests revealed no antigenic relationships to 74 tick-borne arboviruses. The size of the virus is about 100 nm; it agglutinates goose erythrocytes at pH 5.5--7.0, is pathogenic for newborn, 14-day-old and adult white mice and multiplies in primary and continuous cell cultures. Morphological properties of the virus permit its classification as a member of the family Bunyaviridae.
Asunto(s)
Arbovirus/clasificación , Virus Bunyamwera/clasificación , Dermacentor/microbiología , Ovinos/parasitología , Garrapatas/microbiología , Animales , Antígenos Virales/análisis , Armenia , Virus Bunyamwera/aislamiento & purificación , Virus Bunyamwera/fisiología , Pruebas de Fijación del Complemento , Terminología como AsuntoRESUMEN
Strain LEIV-776P, possessing no haemagglutinating activity was isolated in 1971 in the Primorie region (U.S.S.R.) from Haemaphysalis longicornis Neumann 1091 tick. Complement fixation (CF) tests revealed no antigenic relationship with 24 antigenic groups of arboviruses or 21 ungrouped viruses isolated from ticks. The virus isolate contains RNA and is relatively sensitive to ether and sodium deoxycholate; it is pathogenic for suckling mice and two-week-old white mice by the intracerebral route, and replicates in primary cultures of chick and duck fibroblasts and green monkey kidneys and in a continuous line of pig embryo kidney cells without any cytopathic effect. According to electron microscopy, its size is 90-110 nm. Its morphological properties permit to classify it as a member of the family Bunyaviridae.
Asunto(s)
Arbovirus , Virus Bunyamwera , Garrapatas/microbiología , Animales , Antígenos Virales/análisis , Vectores Arácnidos , Arbovirus/clasificación , Virus Bunyamwera/clasificación , Virus Bunyamwera/crecimiento & desarrollo , Virus Bunyamwera/inmunología , Reservorios de Enfermedades , Especificidad de la Especie , U.R.S.S.RESUMEN
Duck embryo cell (DEC) cultures were used for the study of arbovirus reproduction. High sensitivity of DEC cultures to Japanese encephalitis, West Nile, Tyuleniy, Sakhalin and Baku viruses was established. DEC cultures are recommended for investigation of arbovirus reproduction.