RESUMEN
Using developed scheme, complex study of protective properties of avirulent recombinant strain Vibrio cholerae El Tor Inaba KM 184 was performed. Necessity for broadening of standard procedure of testing of cholera vaccines protective properties by using of quantitative methods of assessment of morphological changes and state of biomodel's functional systems, which could increase the information value of assessment of studied vaccines, was experimentally substantiated.
Asunto(s)
Vacunas contra el Cólera/administración & dosificación , Cólera/prevención & control , Cólera/fisiopatología , Estudios de Evaluación como Asunto , Vacunación , Vibrio cholerae O1/inmunología , Administración Oral , Animales , Toxina del Cólera/biosíntesis , Toxina del Cólera/genética , Fragmentos de Péptidos/biosíntesis , Fragmentos de Péptidos/genética , Conejos , Vacunas Atenuadas/administración & dosificación , Vacunas Sintéticas/administración & dosificación , Vibrio cholerae O1/metabolismo , Vísceras/fisiopatologíaAsunto(s)
Antígenos Bacterianos/inmunología , Ratones Endogámicos/inmunología , Yersinia pestis/inmunología , Animales , Femenino , Genotipo , Inmunidad , Inmunización , Ratones , Peste/inmunología , Peste/prevención & control , Vacuna contra la Peste/inmunología , Virulencia , Yersinia pestis/patogenicidadRESUMEN
The influence of Y. pestis phospholipase D on the physiological state of leukocytes in the blood of guinea pigs was studied in vivo by flow impulse fluorometry with the use of fluorochrome acridine orange. During the first hours of observation the intensity of leukocyte fluorescence increased due to a rise in the number of polymorphonuclear leukocytes and changes in the permeability of cell membranes. Further changes in the intensity of the fluorescence of the material under study after 24 hours of observation occurred due to the appearance of activated lymphocytes in the blood stream. The processes normalized by day 21. The reaction of blood leukocytes to phospholipase D was specific in comparison with the reaction to capsular antigen, "mouse" toxin, lipopolysaccharide and the main somatic antigen.
Asunto(s)
Antígenos Bacterianos/toxicidad , Leucocitos/efectos de los fármacos , Leucocitos/inmunología , Fosfolipasa D/toxicidad , Yersinia pestis/enzimología , Yersinia pestis/inmunología , Animales , Citometría de Flujo/instrumentación , Citometría de Flujo/métodos , Cobayas , Leucocitos/química , Fosfolipasa D/aislamiento & purificación , Peste/sangre , Peste/etiología , Peste/inmunología , Factores de Tiempo , Yersinia pestis/patogenicidadRESUMEN
Immunization with live plague vaccine has been shown to give no protection to thymectomized mice from subcutaneous challenge with Y. pestis virulent strain. Under the action of the vaccine or individual Y. pestis antigens (fraction I) the functional and morphological activation of thymocytes and macrophages is observed, more pronounced in C57BL/6 mice and less pronounced in CBA mice. Y. pestis antigenic preparations (fractions I and II, pesticin) act as T-cell mitogens and are thus capable of inducing the in vitro proliferation of thymocytes. At the same time the in vivo action of fraction II induces a decrease in the level of lymphocytes in the peripheral blood of mice and the destruction of lymphocytes in their thymus and spleen.
Asunto(s)
Antígenos Bacterianos/inmunología , Peste/inmunología , Yersinia pestis/inmunología , Animales , Antígenos Bacterianos/toxicidad , Vacunas Bacterianas/inmunología , Vacunas Bacterianas/toxicidad , Haplotipos/inmunología , Inmunidad Celular/inmunología , Inmunización , Ratones , Ratones Endogámicos , Fagocitos/inmunología , Linfocitos T/inmunología , Timo/inmunologíaRESUMEN
Cyclophosphamide injection causes amyloidosis development in the spleen of great gerbils both intact and plague-infected after immunization. The amyloid deposits are observed irrespective of acquired antiplague resistance level. Alterations observed may be considered as acquired (secondary) AA-amyloidosis. Results indicate the significance of immunodepression in amyloidogenesis and a possibility to use the cyclophosphamide-treated great gerbils as a convenient experimental model of amyloidosis.