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ABSTRACT Shark-skin surfaces show the non-smoothness characteristics due to the presence of riblet structures. In this work, biomimetic shark-skins were prepared by means of different bio-replicated forming techniques. These techniques include the PDMS elastomeric stamping method (e.g., PES method) and the PDMS embedded-elastomeric stamping method (e.g., PEES method). The study characterized the fabricated biomimetic surfaces through the use of scanning electron microscopy (SEM) as well as 3D microscope. The accuracy of the two replication routes were compared, which included measuring the surface integrity and dimensional parameters ( s, h ) of the riblet-groove structure. The results show that fresh shark-skin without chemical treatments should be used as a replication template whenever possible to attain a satisfactory replication accuracy of the riblet structure. The PES and PEES methods proposed here are effective bio-replicated forming routes in simulating the microstructures of a shark-skin surface. Compared with the PES method, the PEES method has greater precision in simulating the microstructures of a shark-skin surface.

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Objective To investigate the direct economic loss caused by oral infection in patients with acute leuke-mia.Methods Acute leukemia patients with oral infection in a tertiary first-class hospital in Shandong Province be-tween January 2011 and December 2013 were investigated,the 1 :1 matched case-control method was used for com-paring hospitalization expense and length of hospital stay between oral infected (case group)and uninfected patients (control group ).Results A total of 994 patients with acute leukemia were monitored,277 had healthcare-associated infection,17 (5.56%)of whom were with oral infection.The median hospitalization expense of patients in case group and control group was￥37 327 and￥13 176 respectively,the total hospitalization expense of patients in case group was 2.83 times more than control group,difference was statistically significant (Z = -3.621 ,P <0.001).Each hospitalization expense of case group was higher than control group,especially expense for medicine, blood transfusion,laboratory examination,and therapy.The median length of hospital stay in case group and con-trol group were 17 days and 11 days respectively,rank sum test showed that difference in median length of hospital stay between two groups was statistically significant (Z =-3.627,P < 0.001 ).Conclusion Acute leukemia pa-tients with oral infection have increased hospitalization expense,prolonged length of hospital stay,and increased the financial burden.

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The major source of endogenous hydrogen peroxide is generally thought to be the respiratory chain of bacteria and mitochondria. In our previous works, mesosome structure was induced in cells during rifampicin effect, and the mesosome formation is always accompanied by excess hydrogen peroxide accumulation in bacterial cells. However, the underlying mechanisms of hydrogen peroxide production and the rationale behind it remain still unknown. Here we report that hydrogen peroxide can specifically accumulate in the mesosome in vitro. Mesosomes were interpreted earlier as artifacts of specific cells under stress through TEM preparation, while, in the current study, mesosomes were shown as intracellular compartments with specific roles and features by using quickly freezing preparation of TEM. Formation of hydrogen peroxide was observed in suspension of mesosomal vesicles by using either a fluorescence-based reporter assay or a histochemical method, respectively. Our investigation provides experimental evidence that mesosomes can be a novel site of hydrogen peroxide accumulation.

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Escherichia coli/metabolismo , Peróxido de Hidrógeno/metabolismo , Orgánulos/metabolismo , Antibacterianos/farmacología , Escherichia coli/efectos de los fármacos , Escherichia coli/ultraestructura , Microscopía Electrónica de Transmisión , Orgánulos/ultraestructura , Rifampin/farmacología

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Objective To investigate the economic losses in kidney transplant patients with healthcare-associated lower respiratory tract infection(LRTI).Methods All transplant patients in a hospital from 2008 to 2012 were in-vestigated,patients with LRTI were in infection group(n=45),and patients without LRTI were in control group(n=266),hospitalization expenses and hospitalization days between two groups were compared.Results A total of 383 kidney transplant patients were included in the study,the incidence of LRTI was 11 .75% (n=45),median ex-pense of infection group and control group was ￥79 291 .82 and ￥72 185.14 respectively,the difference was not statistically significant (P >0.05).The largest increased expense in patients with LRTI was medicine (increased by￥5 429.82),medicine and examination expense in infection group were significantly higher than control group (￥39 123.17 vs ￥33 693.35;￥702.52 vs ￥593.73;P <0.05 ).The median hospitalization days in infection group and control group was 28.38 days and 21 .47 days respectively,there was significant difference between two groups(P <0.05 ).Conclusion Kidney transplant patients with LRTI suffer from a heavy financial burden,and their hospitalization days are prolonged,so measures should be taken to prevent the occurrence of LRTI and save limited medical resources.

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Objetive To study the medical value of the combined treatment of laparoscopy and duodenoscopy treatment for calculus of extrahepatic bile duct.Methods Retrospectivly analyzed the clinical data of 72 cases with combination of laparoscopy and duodenoscopy treatment for calculus of extrahepatic bile duct between March 2006 and October 2011.There were 11 cases of choledocholithiasis and 61 cases of choledocholithiasis and cholecystolithiasis.Results Three cases of severe abdominal adhesion converted to laparotomy and other 69 cases were cured.The cure rate was 95.83％.There were no complications such as infection,bile duct injury,bile leakage,intraabdominal hemorrhage,bile reflux cholangitis,pancreatitis and cicatrical stenosis of papilary.The examination after operation showed that there weren't any residual bile duct stones.Conclusions The combination of laparoscopy and duodenoscopy treatment for calculus of extrabepatic bile duct has less traumatic,few intercurrent disease,and was safe and reliable with well postoperative recovery.It can be regarded as the preference treatment for calculus of extrahepatic bile duct.

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Objective To increase externally-assigned response, improve energy response of CR39 and develop positive fast/thermal neutron personal dosimeter applicable for occupational exposure in oil and gas field logging by using pre-recoil layer.Methods The externally-assigned response of CR39 detector was improved through increasing the track density by using the α particle induced by the reaction of 10B(n,α) 7Li with the BN as pre-recoil layer, and the increase was vilified by using both Monte-Carlo simulation and experiment exposed by standard neutron source.Results Fast/thermal neutron personal dosimeter's neutron flux sensitivity and neutron dose equivalent sensitivity were 3.46 × 10-4 track per 0.013 and 52.8 mSv.According to theoretical derivation and experiment of standard 241 Am-Be neutron source, detecting efficiency and energy response of CR39 were effectively improved, and quantitative measurement of dose contributed by thermal neutron was realized.Conclusions CR39 fast/thermal neutron personal dosimeter of high sensitivity is applicable to oil and gas field logging environment and of potential development.