RESUMEN
In order to assess the constituent ratios of energy expenditure in human, the deductive process of establishing a mathematical model for assessing the constituent ratios of the three major nutrients in energy expenditure in human was described in this paper. Total oxygen consumption, total carbon dioxide output and total nitrogen (urine nitrogen + skin nitrogen) excretion are related to the oxidation of the three major nutrients and the physiological combustion values of the three nutrients are already known. Based on these measurable and known parameters, the mathematical model established by the authors will help understand the characteristics of the tested individual's energy expenditure.
Asunto(s)
Metabolismo Energético/fisiología , Modelos Teóricos , Dióxido de Carbono/análisis , Humanos , Fenómenos Fisiológicos de la Nutrición/fisiología , Consumo de OxígenoRESUMEN
OBJECTIVE: To investigate the estrogenic activity of para-nonylphenol in immature SD rats and explore the mechanism and sensitive indicators of its action in uterotrophic assay. METHODS: The vehicle control (peanut oil), positive control(estradiol benzoate, E2B, 0.4 mg/kg) and p-NP(60 mg/kg, 90 mg/kg and 120 mg/kg) were given orally (by gavage) q.d. on the 21st, 22nd, 23rd postnatal days. Then the rats were sacrificed 24 hours after the last administration. By using ABC immunohistochemistry, the progesterone receptor (PR), estrogen receptor (ER), and proliferating cell nuclear antigen (PCNA) of the rat uterine were analysed. RESULTS: Uterine weight, uterine/body weight significantly increased in E2B 0.4 mg/kg, p-NP 90 mg/kg and 120 mg/kg groups as compared with those of vehicle control group (P < 0.01), and a dose-response relationship was observed. The expressions of PR, ER and PCNA in the nuclei of stromal and myometrial cells of uterus were detected in all the p-NP groups, and a dose-effect relationship was noted. CONCLUSION: Expressions of PR, ER and PCNA as indicators tested by immunohistochemical technique are more sensitive than uterine weight in uterotrophic assay. Hyperplasia of stromal and myometrial cells of uterus is the reason why the uterine weight of the rat increased.