RESUMEN
BACKGROUND AND OBJECTIVES: Hidradenitis suppurativa (HS)/acne inversa is an intractable skin disease that is characterized by destructive lesions - primarily on the flexural areas. Although its etiology is unknown, genetics is considered to be a factor of its pathology - mutations in γ-secretase genes have been identified in certain familial HS patients, and follicular occlusion is widely accepted as the primary cause of HS. But, no relationship between these mutations and the components of hair follicles has been reported. Thus, we examined changes in these components in mice with a mutation in NCSTN (a γ-secretase gene). METHODS: We generated C57BL/6 mice with an NCSTN mutation and examined their expression of hair cortex cytokeratin and trichohyalin by Western blot and immunohistochemistry, in addition to nicastrin, the product of NCSTN, and NICD compared with wild-type mice. The structure of hair follicles was analyzed by hematoxylin-eosin staining and transmission electron microscopy. RESULTS: In mice with an NCSTN mutation, HS-like skin lesions appeared after age 6 months, the pathological manifestations of which were consistent with the features of human HS. The structure of hair follicles was abnormal in mice with an NCSTN mutation versus wild-type mice, and hair cortex cytokeratin, trichohyalin, nicastrin, and NICD were downregulated in these mice. CONCLUSIONS: This NCSTN mutant mouse model could be an improved model to study early lesion development aspects of human HS pathogenesis and could perhaps be a better alternative for evaluating early-acting and preventive therapeutics for HS experimentally before clinical trials in HS patients. NCSTN mutations disrupt the development of hair follicles, leading to abnormal hair follicle structures, perhaps resulting in the onset of HS.
Asunto(s)
Folículo Piloso , Hidradenitis Supurativa , Humanos , Animales , Ratones , Lactante , Folículo Piloso/patología , Glicoproteínas de Membrana/genética , Secretasas de la Proteína Precursora del Amiloide/genética , Ratones Endogámicos C57BL , Mutación , Hidradenitis Supurativa/patología , Queratinas/genéticaRESUMEN
Person re-identification (Re-ID) aims to match identities across non-overlapping camera views. Researchers have proposed many supervised Re-ID models which require quantities of cross-view pairwise labelled data. This limits their scalabilities to many applications where a large amount of data from multiple disjoint camera views is available but unlabelled. Although some unsupervised Re-ID models have been proposed to address the scalability problem, they often suffer from the view-specific bias problem which is caused by dramatic variances across different camera views, e.g., different illumination, viewpoints and occlusion. The dramatic variances induce specific feature distortions in different camera views, which can be very disturbing in finding cross-view discriminative information for Re-ID in the unsupervised scenarios, since no label information is available to help alleviate the bias. We propose to explicitly address this problem by learning an unsupervised asymmetric distance metric based on cross-view clustering. The asymmetric distance metric allows specific feature transformations for each camera view to tackle the specific feature distortions. We then design a novel unsupervised loss function to embed the asymmetric metric into a deep neural network, and therefore develop a novel unsupervised deep framework named the DEep Clustering-based Asymmetric MEtric Learning (DECAMEL). In such a way, DECAMEL jointly learns the feature representation and the unsupervised asymmetric metric. DECAMEL learns a compact cross-view cluster structure of Re-ID data, and thus help alleviate the view-specific bias and facilitate mining the potential cross-view discriminative information for unsupervised Re-ID. Extensive experiments on seven benchmark datasets whose sizes span several orders show the effectiveness of our framework.
Asunto(s)
Identificación Biométrica/métodos , Aprendizaje Profundo , Procesamiento de Imagen Asistido por Computador/métodos , Aprendizaje Automático no Supervisado , Algoritmos , Análisis por Conglomerados , Humanos , Grabación en VideoRESUMEN
BACKGROUND: Ketoconazole cream and adapalene gel are effective drugs against pityriasis versicolor. However, there are no reports on combination treatment with both compounds in pityriasis versicolor. OBJECTIVE: To evaluate the efficacy and safety of combination therapy with adapalene 0.1% gel and ketoconazole 2% cream against pityriasis versicolor. METHODS: Participants with pityriasis versicolor were randomly assigned to two groups: the combination group was treated with adapalene 0.1% gel and ketoconazole 2% cream once daily, and the monotherapy group received ketoconazole 2% cream twice daily. The treatment lasted 2 weeks in both groups. Outcomes were assessed at baseline and 1, 2 and 4 weeks after the initiation of treatment. RESULTS: We noted clinically significant differences in total improvement rates between groups Weeks 1 and 2. A statistically significant difference was obtained Week 4. The treatment was well tolerated by all participants. CONCLUSIONS: The combination of adapalene 0.1% gel and ketoconazole 2% cream is effective and safe in the treatment of pityriasis versicolor. This therapeutic regimen was rapid, providing a valuable option for patients with pityriasis versicolor.
Asunto(s)
Adapaleno/administración & dosificación , Cetoconazol/administración & dosificación , Tiña Versicolor/tratamiento farmacológico , Adulto , Quimioterapia Combinada , Femenino , Humanos , Masculino , Adulto JovenRESUMEN
Hyperkeratotic-type tinea pedis is chronic and recalcitrant to topical antifungal agents. Some topical antifungal agents are effective; however, long duration of therapy is required, which often reduce the treatment compliance of patients. To seek for short period therapy of hyperkeratotic type tinea pedis, in this study, we observed the efficacy and safety of treatment of topical terbinafine and 10% urea ointment combined oral terbinafine. Participants with hyperkeratotic type tinea pedis were randomly assigned to two groups. Patients in group I were treated with oral terbinafine for 2 weeks and topical terbinafine and 10% urea ointment for 4 weeks, whereas in group II, only the above topical agents were applied for 12 weeks. Clinical improvement rates and fungal eradication rates were compared between the two groups at 24 weeks after the initiation of treatment. The group I had stopped the topical therapy 8 weeks earlier than group II. There were no significant differences in mycological eradication rates and clinical improvement rates between the two groups, besides, no major side effects were noted in both groups. The short combination therapy with oral terbinafine was effective and safe; it should be a valuable option for patients with hyperkeratotic type tinea pedis.
Asunto(s)
Antifúngicos/administración & dosificación , Naftalenos/administración & dosificación , Tiña del Pie/tratamiento farmacológico , Urea/administración & dosificación , Administración Oral , Administración Tópica , Adulto , Anciano , Antifúngicos/efectos adversos , Quimioterapia Combinada/efectos adversos , Quimioterapia Combinada/métodos , Femenino , Humanos , Masculino , Persona de Mediana Edad , Naftalenos/efectos adversos , Pomadas/administración & dosificación , Estudios Prospectivos , Terbinafina , Resultado del Tratamiento , Urea/efectos adversos , Adulto JovenRESUMEN
BACKGROUND: Ketoconazole is a typical treatment available for pityriasis versicolor; tretinoin cream is effective, too. Adapalene gel is a tretinoin derivative and has a lower incidence of irritation compared with other topical retinoid products. However, there are no reports on adapalene gel for the treatment of pityriasis versicolor. OBJECTIVE: To study the effect of adapalene gel comparing the treatment with adapalene gel and 2% ketoconazole cream in pityriasis versicolor. METHODS: Eighty patients suffering from pityriasis versicolor were randomly divided into two groups; one group were treated with 2% ketoconazole cream topically twice daily for 2 weeks, adapalene gel was used for the other group in a similar fashion. RESULTS: There were no significant differences in efficacy between the two groups. No major side effects were noted in any of the groups either. CONCLUSION: Adapalene was the favorable option in the treatment of pityriasis versicolor. The probable therapeutic mechanism of adapalene is also discussed.