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Biol. Res ; 49: 1-11, 2016. ilus, graf
Artículo en Inglés | LILACS | ID: biblio-950867

RESUMEN

BACKGROUND: Asthma is an increasing global health problem, and novel strategies to prevent or ameliorate the condition are needed. Here, the effects of 80 % ethanol extracts of Salvia plebeia R. Br. (SE) on an induced inflammatory response were investigated RESULTS: Salvia plebeia R. Br. inhibited production of pro-inflammatory cytokines, such as TNF-α and IL-6, as well as nitric oxide (NO) in LPS-stimulated RAW 264.7 cells. NO and pro-inflammatory cytokine production was suppressed more effectively by SE of the aerial parts (SE-A) than of the roots (SE-R) of S. plebeia. In BEAS-2B cells, both SE-A and SE-R inhibited the increase in production of the inflammatory cytokines IL-6 and IL-8. We also investigated the antiasthmatic effects of SE in an ovalbumin (OVA)-induced BALB/c mouse model. SE-A treatment significantly reduced the number of airway eosinophils, IL-4 and IL-13 levels, mucus production, and inflammatory infiltration, as compared with the corresponding levels in the untreated, OVA-induced mice, and had similar effects to dexamethasone CONCLUSIONS: Salvia plebeia ethanol extract ameliorated the induced inflammatory response in RAW 264.7 and BEAS-2B cells, with more effective inhibition noted for SE-A than for SE-R. SE-A treatment was effective in improving the histopathological changes in the lungs of asthma model mice via modulation of eosinophils and Th2 cytokines. These results suggest that SE-A can be considered as a therapeutic agent that can potentially relieve asthma


Asunto(s)
Animales , Femenino , Ratones , Asma/tratamiento farmacológico , Medicamentos Herbarios Chinos/farmacología , Antiasmáticos/farmacología , Antiinflamatorios/farmacología , Asma/inducido químicamente , Ensayo de Inmunoadsorción Enzimática , Células Cultivadas , Ovalbúmina , Lipopolisacáridos/farmacología , Reproducibilidad de los Resultados , Citocinas/análisis , Citocinas/efectos de los fármacos , Componentes Aéreos de las Plantas/química , Modelos Animales de Enfermedad , Etanol/farmacología , Reacción en Cadena en Tiempo Real de la Polimerasa , Células RAW 264.7 , Pulmón/efectos de los fármacos , Pulmón/fisiología , Ratones Endogámicos BALB C , Óxido Nítrico/análisis
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