Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Más filtros











Base de datos
Intervalo de año de publicación
1.
J Agric Food Chem ; 59(13): 6856-63, 2011 Jul 13.
Artículo en Inglés | MEDLINE | ID: mdl-21604714

RESUMEN

Because of the increasing use of maize hybrids with genetically modified (GM) stacked events, the established and commonly used bulk sample methods for PCR quantification of GM maize in non-GM maize are prone to overestimate the GM organism (GMO) content, compared to the actual weight/weight percentage of GM maize in the grain sample. As an alternative method, we designed and assessed a group testing strategy in which the GMO content is statistically evaluated based on qualitative analyses of multiple small pools, consisting of 20 maize kernels each. This approach enables the GMO content evaluation on a weight/weight basis, irrespective of the presence of stacked-event kernels. To enhance the method's user-friendliness in routine application, we devised an easy-to-use PCR-based qualitative analytical method comprising a sample preparation step in which 20 maize kernels are ground in a lysis buffer and a subsequent PCR assay in which the lysate is directly used as a DNA template. This method was validated in a multilaboratory collaborative trial.


Asunto(s)
ADN de Plantas/análisis , Plantas Modificadas Genéticamente/genética , Reacción en Cadena de la Polimerasa/métodos , Semillas/genética , Zea mays/genética , Reproducibilidad de los Resultados , Zea mays/clasificación
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA