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Chemotherapy ; 51(1): 21-6, 2005 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-15722629

RESUMEN

BACKGROUND: In order to identify intraspecific variations in Trichophyton rubrum and to correlate them to the immunological status of the host, sixty strains isolated from AIDS, HIV-positive and HIV-negative patients were compared for the production of extracellular enzymes and for their susceptibility to several antifungal drugs. METHODS: The isolates were tested for their ability to secrete keratinases, proteinases, phospholipases, lipases and DNases. Likewise, we investigated their susceptibility to amphotericin B, ketoconazole, ciclopiroxolamine, griseofulvin, miconazole and tolnaftate. RESULTS: Variations in the Minimal Inhibitory Concentration (MIC80)) values were observed for all antifungals tested, but they were similarly distributed among the three clinical groups. Griseofulvin showed the most prominent differences among the three groups of isolates. Regarding enzyme secretion, all samples secreted keratinases and DNases, while none secreted phospholipases. Proteinases and lipases were secreted by some of them. CONCLUSIONS: The differences among isolates of the three groups were not statistically significant and therefore could not be ascribed to a given clinical status. Intraspecific variations similarly occurred in each group, irrespective of the immunological status of the patients.


Asunto(s)
Síndrome de Inmunodeficiencia Adquirida/microbiología , Antifúngicos/farmacología , Tiña/microbiología , Trichophyton/efectos de los fármacos , Trichophyton/enzimología , Brasil/epidemiología , Desoxirribonucleasas/metabolismo , Susceptibilidad a Enfermedades , VIH/patogenicidad , Humanos , Lipasa/metabolismo , Pruebas de Sensibilidad Microbiana , Péptido Hidrolasas/metabolismo , Fosfolipasas/metabolismo , Trichophyton/aislamiento & purificación
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