RESUMEN
Detailed mechanisms on the effect of oxidative stress (OS), an etiological factor involved in photocarcinogenesis, remain to be fully elucidated. We used immunohistochemical methods to study OS in the DNA, protein and lipids of patients with actinic keratosis (AK), Bowen's disease (BD) and squamous cell carcinoma (SCC). Between January 2009 and December 2014, we treated 230 patients; 79 had AK, 61 had (BD) and 90 had cutaneous SCC; 28 healthy subjects served as the normal controls. OS on DNA, protein and lipids was assessed by the expression of 8-hydroxydeoxyguanosine (8-OHdG), dityrosine (DT) and malondialdehyde (MDA), respectively. 8-OHdG was significantly overexpressed in AK and BD lesions compared with surrounding non-lesional tissue, SCC lesions and the healthy controls. DT was more highly expressed in AK, BD and SCC than in the controls. There was no significant difference among AK, BD and SCC. The expression of MDA was higher in AK, BD and SCC lesions than the controls; SCC showed the highest expression. Our observations suggest that DNA oxidation plays an important role in the early stage of carcinogenesis, that protein oxidation is involved in all stages of carcinogenesis and that lipid oxidation is strongly implicated in the late stages of carcinogenesis.
Asunto(s)
Biomarcadores de Tumor/metabolismo , Enfermedad de Bowen/patología , Queratosis Actínica/patología , Estrés Oxidativo , Neoplasias Cutáneas/patología , Adulto , Anciano , Anciano de 80 o más Años , Biopsia , ADN/metabolismo , Femenino , Humanos , Inmunohistoquímica , Metabolismo de los Lípidos , Masculino , Persona de Mediana Edad , Oxidación-Reducción , Piel/patologíaRESUMEN
Autoimmune autonomic ganglionopathy (AAG), clinically characterized by gastrointestinal dysmotility, orthostatic hypotension and tonic pupils, is an idiopathic acquired disorder of the autonomic nervous system elicited by antibodies against ganglionic acetylcholine receptor (gAChR). We encountered a 60-year-old man who presented with severe anhidrosis, difficulty in thermoregulation, orthostatic hypotension, gastrointestinal dysmotility, tonic pupils and ptosis. Histologically, an anhidrotic skin sample was normal. Routine laboratory examinations of blood, urine and cerebrospinal fluid returned no abnormal findings. Serological examination revealed antibodies against α3 and ß4 subunits of gAChR. The diagnosis was AAG. As sudomotor dysfunction reflects ganglionic neuropathy in AAG, we concluded that his anhidrosis was attributable to AAG. Anhidrosis is an important clue for the diagnosis of AAG, a rare neurological disorder.
Asunto(s)
Autoanticuerpos/sangre , Enfermedades Autoinmunes del Sistema Nervioso/complicaciones , Ganglios Autónomos/inmunología , Hipohidrosis/etiología , Proteínas del Tejido Nervioso/inmunología , Receptores Nicotínicos/inmunología , Autoanticuerpos/inmunología , Enfermedades Autoinmunes del Sistema Nervioso/sangre , Enfermedades Autoinmunes del Sistema Nervioso/inmunología , Enfermedades Autoinmunes del Sistema Nervioso/terapia , Blefaroptosis/sangre , Blefaroptosis/etiología , Blefaroptosis/terapia , Ganglios Autónomos/efectos de los fármacos , Ganglios Autónomos/patología , Enfermedades Gastrointestinales/sangre , Enfermedades Gastrointestinales/etiología , Enfermedades Gastrointestinales/terapia , Motilidad Gastrointestinal/efectos de los fármacos , Glucocorticoides/uso terapéutico , Humanos , Hipohidrosis/sangre , Hipohidrosis/terapia , Hipotensión Ortostática/sangre , Hipotensión Ortostática/etiología , Hipotensión Ortostática/terapia , Inmunoglobulinas Intravenosas/uso terapéutico , Masculino , Persona de Mediana Edad , Plasmaféresis , Prednisolona/uso terapéutico , Piel/inervación , Piel/patología , Insuficiencia del TratamientoRESUMEN
Pemphigus vulgaris (PV) is an autoimmune blistering disease elicited by anti-desmoglein (DsG) 3 antibody. Although skin lesions tend to be distributed over the entire body, in some patients, they are confined to a restricted area. We report two patients who presented with long-lasting localized PV without detectable anti-DsG antibodies after suffering antibody-positive systemic PV. Initial treatment with prednisolone (PSL) was successful in both patients, but a local relapse occurred on the cheek or lower lip after a reduction in the PSL dose. Biopsy of the localized lesions showed suprabasal acantholysis; no serum DsG antibodies were found. Local immunosuppression therapy was effective in both patients. Based on our findings, we suggest that localized PV without detectable antibodies can develop after systemic PV.
RESUMEN
We previously reported the clinical effectiveness of transdermal nicotine patches for the treatment of skin disorders with eosinophilic infiltration such as Kimura's disease, erythema nodosum and eosinophilic pustular folliculitis (EPF). We assessed the efficacy and safety of transdermal nicotine patches for EPF. We treated eight patients with EPF with transdermal nicotine patches and evaluated the treatment response by performing overall lesional assessment. Excellent 77and good responses were obtained in five and one patient(s), respectively. In the other two patients, the lesions remained unchanged. No severe adverse effects were observed. Our results suggest that transdermal nicotine patches may be useful and safe in the treatment of EPF.