RESUMEN

Oryzias latipes, Danio rerio, Cyprinus carpio, and Zacco platypus are useful indicator species for CYP1A biomarker studies; however, comparative studies have not been performed. To compare susceptibility, dose- and time-dependent CYP1A induction at the mRNA and protein levels in response to benzo(a)pyrene (BaP) exposure was analyzed. At the mRNA level, a statistically significant difference was found among the four species; however, such was not observed at the protein level. C. carpio showed the highest CYP1A induction level and the steepest slope in the dose-response curve. To assess susceptibility, the difference in CYP1A mRNA induction among species must be considered, and C. carpio was the most sensitive species of the four evaluated in terms of CYP1A expression.

Asunto(s)

Benzo(a)pireno/efectos adversos , Cyprinidae/crecimiento & desarrollo , Citocromo P-450 CYP1A1/genética , Citocromo P-450 CYP1A1/metabolismo , Oryzias/crecimiento & desarrollo , Animales , Benzo(a)pireno/farmacología , Carpas/genética , Carpas/crecimiento & desarrollo , Carpas/metabolismo , Cyprinidae/clasificación , Cyprinidae/genética , Cyprinidae/metabolismo , Relación Dosis-Respuesta a Droga , Contaminantes Ambientales/efectos adversos , Contaminantes Ambientales/farmacología , Proteínas de Peces/genética , Proteínas de Peces/metabolismo , Regulación Enzimológica de la Expresión Génica/efectos de los fármacos , Branquias/enzimología , Hígado/enzimología , Oryzias/genética , Oryzias/metabolismo , Filogenia , Pez Cebra/genética , Pez Cebra/crecimiento & desarrollo , Pez Cebra/metabolismo