RESUMEN

AIM: To explore the mechanisms by which splicing factor SC35 regulates the costimulatory molecule B7-H3 expression in vitro through bioinformatic and molecular biological methods. METHODS: We screened some regulatory proteins which might take part in regulating B7-H3 expression using bioinformatic methods. Then RNA interference (RNAi) and real-time PCR were performed to test if these proteins took part in the regulation. RESULTS: Splicing factor SC35, SRP40 and SF50 might play an important role in the regulation of B7-H3 expression. In PHA-activated T cells, B7-H3 was upregulated obviously and at the same time SC35 was also upregulated. When we suppressed SC35 expression using RNAi, we found B7-H3 was also downregulated. CONCLUSION: Splicing factor SC35 might take part in the regulation of B7-H3 expression, which could help understand B7-H3 biological function.

Asunto(s)

Antígenos B7/genética , Regulación de la Expresión Génica , Proteínas Nucleares/metabolismo , Ribonucleoproteínas/metabolismo , Antígenos B7/metabolismo , Células Cultivadas , Humanos , Activación de Linfocitos/genética , Interferencia de ARN , Proteínas de Unión al ARN/metabolismo , Factores de Empalme Serina-Arginina , Linfocitos T/metabolismo