RESUMEN

Circulating immune complexes (CIC) from 15 paracoccidioidomycosis (PCM) patient sera and from 20 healthy control sera were analysed. After CIC precipitation, solubilization and acid treatment, only a little reactivity to P. brasiliensis antigens was found in the free antibodies from PCM-CIC. This result has suggested that there were antibodies with a high affinity bound to fungus components. Dissociated CIC were fractionated in a column of Sephacryl S300 and the fractions that probably contained antigens were pooled and applied to an affinity column, prepared with mouse anti-gp43 monoclonal antibody. Using ECL-Western blotting assay two polypeptide with apparent mass of 43 and 62 kDa were found.

Asunto(s)

Complejo Antígeno-Anticuerpo/sangre , Paracoccidioidomicosis/inmunología , Adulto , Anciano , Animales , Anticuerpos Antifúngicos/sangre , Anticuerpos Monoclonales , Complejo Antígeno-Anticuerpo/aislamiento & purificación , Antígenos Fúngicos/sangre , Humanos , Inmunoglobulina G/sangre , Inmunoglobulina M/sangre , Ratones , Persona de Mediana Edad , Paracoccidioidomicosis/sangre , Valores de Referencia

Asunto(s)

Streptococcus mutans , Inmunoglobulina A , Caries Dental , Placa Dental