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Objective: This current study aimed to validate the Indonesian version of the Inventory of Statements About Self-Injury (ISAS) questionnaire, which provides a better understanding of Non-Suicidal Self-Injury (NSSI) disorders. Method : The study used a cross-sectional design and involved 314 adolescents and young adults in high school or university. A stratified sampling method was used. All participants filled out the ISAS questionnaire. Data were analyzed for content validity, construct validity, item discrimination value, and internal consistency (Croncbach's α). The translation process was carried out using forward and back-translation methods. Results: The ISAS questionnaire consists of section I, the behavioral scale, and section II, the functional scale. For content validity, Aiken's V coefficient obtained for both scale sections I and II is in the range of 0.917 - 1. This result shows that all items on the scale have very good validity. Confirmatory Factor Analyses were carried out using Lisrel 8.80 software on section II, resulting in several goodness of fit values that were not good enough (χ2 = 457.68; P < 0.000; df = 64; χ2 / df = 7.151, RMSEA = 0.130) and several other values that are quite acceptable (CFI = 0.95; SRMS = 0.057; NFI = 0.95; GFI = 0.83). The factor loading from section II ranges from 0.43 - 0.91. The item discrimination value using the corrected item-total correlation of section I is in the range of 0.031 - 0.837 and section II ranges from 0.290 - 0.854. The reliability analysis values in section I and II of the ISAS are α = 0.527 and α = 0.966, respectively. Conclusion: This is the first study to have validated the Indonesian version of the ISAS questionnaire. The Indonesian version of the ISAS questionnaire is considered a valid and reliable instrument to assess NSSI disorders.
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Introduction: Our study aims to observe the effect of mesenchymal stem cells (MSCs) in lowering amyloid accumulation and ryanodine receptor 3 (RYR3) gene expression, further improving cognitive dysfunction in Alzheimer's disease (AD). Methods: Twenty male adult Wistar rats were randomly distributed into three groups of animals (n = 5). The AlCl3 group was given 300 mg/kg body weight (BW) of AlCl3 intraperitoneally for 5 days, further the MSC injection, and their effect after 30 days was observed. Results: MSCs improved amyloid accumulation and Y-maze scores, and expression of the RYR3 gene decreased compared to the control group. Conclusions: MSCs improved amyloid accumulation, Y-maze scores, and RYR3 expression in the AD animal model.
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INTRODUCTION: Metalloproteinases-3 (MMP3) are the main enzymes involved in cartilage degradation. Several genetic and non-genetic factors can increase the expression of MMP3 in patients with osteoarthritis (OA). This study aims to analyze the risk factors associated with the expression of the MMP3 gene rs679620 fluid synovial knee OA patients. METHODS: A cross-sectional study was conducted at the orthopedic polyclinic Arifin Achmad Riau Province and Ibn Sina Hospital in Pekanbaru City. Ninety women who experienced knee OA were taken as samples by consecutive sampling and then signed the informed consent. Data were obtained through interviews using a questionnaire about characteristics, followed by weight and height measurements. Interleukin-1 ß (IL-1ß) and Tumor Necrosis Factor (TNF-α) were examined from the synovial fluid using the enzyme-linked immunosorbent assay (ELISA) method. The Metalloproteinases-3 (MMP3) gene polymorphism rs679620 was obtained from the DNA analysis of joint fluid results in the Biomedical Laboratory of the Faculty of Medicine, Andalas University. The data was processed computerized and then analyzed using the correlation Spearman-Rank, and chi-square tests. The results of statistical analysis are considered significant if the p-value is 0.05. RESULTS: The MMP3 rs679620 gene polymorphism of the mutant type was 88.9%, with the same proportion of AG and GG alleles (44.4%). Subjects aged ≥ 60 years were 53.3%, 85.6% did not work and 84.4% had menopause. The highest degree of OA was grade 2 (53.3%), most of whom had a risky nutritional status (84.4%). The median expression of the MMP3 rs679620 gene was 5.28 copies number. There is a significant relationship between MMP3 gene polymorphism rs679620, age, IL-1ß, and TNF-α with MMP3 gene expression rs679620. There is no significant relationship between BMI, work status, and menopausal status with MMP3 gene expression rs679620. Conclusion. MMP3 gene polymorphism rs679620, age, levels of IL-1ß and TNF-α are risk factors for increased MMP3 gene rs679620 expression in female knee OA.