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Objective To observe the protective effects of Bushen Jiedu Recipe (BJR) on radia- tion induced hematopoietic injury. Methods C57BL/10J mice TLR4 gene (TLR4 +/+) and knockout mice (TLR4 -/-) were randomly divided into 3 groups, the blank control group, the radiation model group, the BJR group. Sampling was respectively performed at day 1 , 14, and 30 after radiation. The general condi- tion of mice was observed. White blood cell (WBC) , red blood cell ( RBC) , and platelet ( PLT) were counted in each group. Thigh bone of mice was collected for bone marrow specimen. Bone marrow slice was prepared. Pathomorphological changes were observed under electron microscope. Results Com- pared with the blank control group, WBC, RBC, and PLT all decreased or showed a decreasing tendency after one day radiation. RBC and PLT significantly decreased after 14 days of radiation (P <0. 01 , P < 0. 05). Counts of WBC and RBC were higher in the TLR4 +/+ BJR group than in the rest 2 groups (P < 0. 01 , P <0. 05). No statistical difference in RBC or PLT between mice after 30 days of radiation and mice in the blank control group (P >0. 05). PLT count in the TLR4 +/+ BJR group was most approximate to the normal value. WBC count obviously increased, but still with statistical difference as compared with the blank control group (P <0.01). WBC count recovered most rapidly in the TLR4 +/+ BJR group. Results under light microscope showed the structure of bone marrow was injured to different degrees in all mice except those in the TLR4 +/+ BJR group. Conclusion BJR could attenuate radiation induced hematopoi- etic injury possibly through TLR4 signal pathway, thus playing significant radioprotective roles.

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OBJECTIVE: To investigate the correlation between basic Chinese medicine (CM) syndromes (deficiency and excess syndromes) and intracranial neurotransmitter levels in primary insomnia (PI), to provide objective indicators and syndrome-based medical evidence for the differentiation of PI. METHODS: A total of 158 patients with PI were recruited for CM syndrome differentiation. Another 30 healthy people without sleep disorders were selected as control group. An encephalofluctuograph analyzer was used to test the levels of intracranial neurotransmitters, including γ- aminobutyric acid (GABA), glutamate (Glu), 5-hydroxytryptamine (5-HT), dopamine (DA), etc., and their relevance were analyzed. RESULTS: The neurotransmitter levels in the basic-deficiency group were lower than those in the healthy-control group, while the basic-excess group had higher levels than the healthy-control and basic-deficiency groups. Among the neurotransmitters, the 5-HT level was higher in the basic-excess group than in the basic-deficiency group (24.20±4.07 vs. 21.13±3.23; P<0.05); for the intermingled deficiency-excess group, the level of GABA was higher than that in the basic-deficiency group (9.48±3.07 vs. 7.23±3.67; P<0.05), Glu level was higher than that in the healthy-control group (7.53±4.10 vs. 5.83±0.99, P<0.05), and 5-HT and DA levels were lower than those in the healthy-control group (19.80±5.68 vs. 22.63±3.31, 5.27±3.79 vs. 6.83±1.58, respectively; P<0.05). CONCLUSIONS: There was a correlation between the basic syndromes and intracranial neurotransmitter levels in patients with PI, which could objectively reflect the CM differentiation in PI. This information could be important for improving CM diagnosis and treatment in PI.

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OBJECTIVE: To evaluate the effect mechanism of warm acupuncture combined with auricular point sticking therapy and its efficacy on insomnia by monitoring the level of brain neurotransmitters in the insomnia patients. METHODS: One hundred and thirty patients with insomnia were randomized into an observation group and a control group, 65 cases in each one. In the observation group, based on the treating principle of warming yang and benefiting qi, acupuncture was applied to Xinshu (BL 15), Pishu (BL 20), Shenshu (BL 23), Yaoyangguan (GV 3), Baihui (GV 20), Neiguan (PC 6) and Shenmen (HT 7). Warm acupuncture was supplemented at Xinshu (BL 15), Pishu (BL 20), Shenshu (BL 23) and Yaoyangguan (GV 3). The treatment was given once every day. In the control group, estazolam tablets, 0. 5 to 1 mg were prescribed for oral administration, 30 min before sleep at night. The treatment of 14 days was taken as one session and 2 sessions were required in the two groups. The encephal of luctuograph technology was used to observe the sleep quality and brain neurotransmitters before and after treatment in the two groups and the efficacy was compared between the two groups. RESULTS: The total effective rate was 83.1% (54/65) in the observation group and 87.7% (57/65) in the control group. The efficacy was similar between the two groups (P > 0.05). In the observation group, after treatment, 5-HT and GABA/Glu were all increased compared with those before treatment (P < 0.05, P < 0.01) and norepinephrine (NE) was reduced compared with that before treatment (P < 0.05). The level of each index did not change significantly before and after treatment in the control group (all P > 0.05). The regulations of 5-HT, GABA/Glu in the observation group were superior to those in the control group (all P < 0.05). CONCLUSION: The combined therapy of warm acupuncture and auricular point sticking method for warming yang and benefiting qi effectively improves brain neurotransmitters and essentially improves sleep quality of the patients with insomnia differentiated as yang deficiency pattern.

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