RESUMEN

OBJECTIVE: To determine the effect of nylestriol and levonorgestrel on mRNA expression of OPG/OPGL in MG-63 cell lines, and to explore whether the paracrine effect is involved in the regulation course. Methods Semi-quantitive RT-PCR was conducted to analyze mRNA expression of OPG/OPGL in MG-63 cell lines treated with nylestriol and levonorgestrel. Results Both medicines could stimulate MG-63 cell lines to express OPG, and the best action levels of the two medicines were 10(-6) mol/L. Both medicines could decrease mRNA expression levels of OPGL dramatically. In NYL 10(-10) mol/L treatment group, medium renewal has negative effect on mRNA expression of OPG. Conclusion Both the two medicines can promote mRNA expression of OPG in MG-63 cell lines, while decrease mRNA expression of OPGL. The paracrine effect of MG-63 cell lines may be involved in the regulation of mRNA expression of OPG by NYL.

Asunto(s)

Proteínas Portadoras/biosíntesis , Glicoproteínas/biosíntesis , Levonorgestrel/farmacología , Glicoproteínas de Membrana/biosíntesis , Osteosarcoma/patología , Quinestrol/análogos & derivados , Receptores Citoplasmáticos y Nucleares/biosíntesis , Receptores del Factor de Necrosis Tumoral/biosíntesis , Proteínas Portadoras/genética , Femenino , Glicoproteínas/genética , Humanos , Glicoproteínas de Membrana/genética , Osteoprotegerina , Quinestrol/farmacología , Ligando RANK , ARN Mensajero/biosíntesis , ARN Mensajero/genética , Receptor Activador del Factor Nuclear kappa-B , Receptores Citoplasmáticos y Nucleares/genética , Receptores del Factor de Necrosis Tumoral/genética , Células Tumorales Cultivadas