RESUMEN
The feasibility of applying bio-augmentation tactics to remit the influence of transient oxytetracycline (OTC) shock on the anaerobic ammonium oxidation (ANAMMOX) process was evaluated. The bio-augmentation was applied together with shock test, with OTC shock concentration of 518 mg L(-1) and 1-h duration. 0.655-2.62 g volatile suspended solid (VSS) sludges were varied to optimize bio-augmentation dosage (BAD), and appropriate bio-augmentation time (BAT) was determined. The validity of the bio-augmentation was indicated by recovery performance and sludge characteristics. The restoring time of 38 h for bio-augmented reactor was shorter than that of non-bio-augmented reactor (45 h), and heme c content was increased respectively from 0.195 ± 0.001, 0.267 ± 0.047, 0.301 ± 0.049, to 0.340 ± 0.053 µmol g(-1) VSS with the BAD of 0.655, 1.31, 1.97, 2.62 g-VSS. The results suggest that bio-augmentation enhances the recovery of ANAMMOX performance following OTC shock and BAT and BAD are key operational factors.
Asunto(s)
Compuestos de Amonio/metabolismo , Oxitetraciclina/metabolismo , Anaerobiosis , Reactores Biológicos , Hemo/análogos & derivados , Hemo/metabolismo , Nitrógeno/aislamiento & purificación , Oxidación-Reducción , Polímeros/metabolismo , Aguas del AlcantarilladoRESUMEN
The effect of transient oxytetracycline (OTC) shock on the stability of anaerobic ammonium oxidation (ANAMMOX) process was evaluated in the present study. The shock test was implemented with 155-1731mgL(-1) OTC, lasting for 1 to 3-fold hydraulic retention times, under the nitrogen loading rate (NLR) of 6.72 and 13.4kgm(-3)d(-1). The response of the process was divided into shock and recovery stage and the performance under the stress was indicated by stability index and granule characteristic. In the shock period, nitrogen removal rate (NRR) was ranged from 12.1 to 12.1-4.04kgm(-3)d(-1). The specific ANAMMOX activity (SAA) and heme c content, were respectively reduced by 1.4% and 17.6-29.4%. Foremost, the OTC shock was restorable and the recovery lasted for 4-353h. The robustness of ANAMMOX process was dependent on OTC level, duration of shock and NLR applied.