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In the outbreak of COVID-19,triage procedures based on epidemiology were implemented in a local hospital in Changsha to control the transmission of SARS-CoV-2 and avoid healthcare-associated infection.This re-trospective study analyzed the data collected during the triage period and found that COVID-19 patients were en-riched 7 folds into the Section A designated for patients with obvious epidemiological history.On the other side,nearly triple amounts of visits were received at the Section B for patients without obvious epidemiological history.8 COVID-19 cases were spotted out of 247 suspected patients.More than 50％of the suspected patients were submi-tted to multiple rounds of nucleic acid analysis for SARS-CoV-2 infection.Of the 239 patients who were diagnosed as negative of the virus infection,188 were successfully revisited and none was reported as COVID-19 case.Of the 8 COVID-19 patients,3 were confirmed only after multiple rounds of nucleic acid analysis.Besides comorbidities,delayed sharing of epidemiological history added complexity to the diagnosis in practice.The triaging experience and strategy will be helpful for the control of infectious diseases in the future.
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Objective To establish the animal model of diffuse cortical dysplasia in Sprague-Dawley rats. Methods Pregnant rats were given intraperitoneal injections of BCNU on embryonic day 17 (E17). Cresyl-violet staining was applied to observe the histological changes in the cortex, hippocampus and neurons of the resulted pups at P60. EEG recordings were detected, and daily activities were observed. Hot water bath was used to induce seizures, the latency to seizure onset and duration of SE (epileptic status) were compared. Learning and memory abilities were estimated with Y maze at different time points. Results The mean wet brain weights in the BCNU-exposed pups were lower than those of controls on P0 (P＜0.01). Cresyl-violet staining revealed disruption of cortical lamination and heterotopic cell clusters within the hippocampus. Daily activities were poor in BCNU-exposed pups. No obvious epilepsia discharges were detected. After being induced seizures, adult rats with cortical dysplasia had shorter latency to seizures(P＜0.01). The frequency of attempting learning and memory of rats in model group was increased than that in normal group (P＜0.05). Conclusions Intraperitoneal injections of BCNU on embryonic day 17 (E 17) can establish the animal model of diffuse cortical dysplasia, and this model has increased seizure susceptibilities and cognitive functional impairments.
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<p><b>OBJECTIVE</b>To study the clinical and genetic characteristics of a Chinese family with benign familial convulsions (BFNC).</p><p><b>METHODS</b>The clinical data of this family was analyzed. The blood samples were collected from 13 members of this family. By four microsatellite markers which are located in the gene loci of both K+ channel KCNQ2 and KCNQ3, the linkage analysis was performed in the family. With DNA direct sequencing and restriction endonuclease cutting analysis, the mutation analysis of KCNQ3 gene was made for the proband, other 12 family members and 76 unrelated normal individuals.</p><p><b>RESULTS</b>There were 7 patients with BFNC observed in the three generation of family. The BFNC seizures of all patients disappeared during one month and no recurrence of seizures was found. The linkage analysis suggested the disease gene linked to KCNQ3 gene locus in the family. The mutation 988(C to T) of KCNQ3 gene was found in the proband by DNA-direct sequencing. Cosegregation of this mutation with BFNC was confirmed by restriction endonuclease cutting analysis.</p><p><b>CONCLUSION</b>Chinese patients with BFNC can be caused by KCNQ3 gene mutation.</p>