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Zhonghua Nan Ke Xue ; 16(1): 34-9, 2010 Jan.
Artículo en Chino | MEDLINE | ID: mdl-20180402

RESUMEN

OBJECTIVE: To evaluate the effects of red clover isoflavones on the proliferation and apoptosis of human benign prostatic hyperplasia (BPH) stromal cells. METHODS: We treated human prostate stromal cells with red clover isoflavones at the concentration of 12.5, 25, 50 and 100 microg/ml, and established a PBS blank control, a dimethyl sulphoxide (DMSO) negative control and four finasteride positive control groups (at the concentration of 12.5, 25.0, 50.0 and 100.0 microg/ml). We determined the effects of different concentrations of red clover isoflavones on the proliferation of the cells by MTT assay and on their apoptosis by Annexin V/PI double staining flow cytometry. RESULTS: Red clover isoflavones inhibited the proliferation of the BPH stromal cells by 18.86% at 25.0 microg/ml, compared with 5.17% in the blank control group (P < 0.05), and more obviously at a higher concentration. At 50.0 microg/ml, red clover isoflavones exhibited a weaker inhibitory effect than finasteride (28% vs 69.88% , P < 0.05). Annexin V/PI double staining flow cytometry showed that red clover isoflavones at 25.0 microg/ml induced the apoptosis of the prostate stromal cells by (18.54 +/- 2.5)%, with significant differences from the negative control and blank control (P < 0.01). CONCLUSION: Red clover isoflavones can inhibit the proliferation and promote the apoptosis of human BPH stromal cells.


Asunto(s)
Apoptosis/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Isoflavonas/farmacología , Extractos Vegetales/farmacología , Hiperplasia Prostática/patología , Células Cultivadas , Humanos , Isoflavonas/uso terapéutico , Masculino , Extractos Vegetales/uso terapéutico , Próstata/citología , Próstata/efectos de los fármacos , Hiperplasia Prostática/tratamiento farmacológico , Células del Estroma/efectos de los fármacos , Trifolium/química
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