RESUMEN
OBJECTIVE: Multidrug resistance and toxicity significantly compromise the therapeutic efficacy for sarcomas. We aimed at evaluating the effect of lutein-doxorubicin (DOX) combinatorial therapy on inhibiting S180 (Sarcoma 180) cell proliferation and tumor growth. MATERIALS AND METHODS: S180 cells in logarithmic growth phase were treated with lutein, DOX, or lutein-DOX combinatorial therapy for 48 h. The cell survival rate was determined by MTT assay. Apoptosis was detected by flow cytometry. The expression of PCNA, P53, and NF-κB was assessed by Western blot. Further, mice bearing S180 tumors received lutein, DOX, or lutein-DOX combinatorial therapy by oral gavage. RESULTS: Lutein-DOX combinatorial therapy significantly decreased the proliferation of S180 cells (p<0.01) in vitro. Also, the expression of proliferating cell nuclear antigen (PCNA) (p<0.05) and the apoptosis-relevant gene p53 were decreased, which resulted in increased cell apoptosis (p<0.05). The level of nuclear factor kappa B (NF-κB) was also decreased by the combinatorial therapy. Lutein-DOX combinatorial therapy reduced the cytotoxicity of DOX and reduced the inflammatory response. The inhibitory effect of lutein-DOX combinatorial therapy on cell proliferation was confirmed in vivo. The growth rate and size of the tumor at 30 d after treatment were significantly lower than those of the control group and DOX single therapy. CONCLUSIONS: Lutein and DOX synergistically inhibit sarcoma cell proliferation and tumor growth. This novel therapeutic regimen could potentially improve clinical outcome of sarcoma patients.
Asunto(s)
Doxorrubicina/administración & dosificación , Luteína/administración & dosificación , Sarcoma 180/tratamiento farmacológico , Animales , Apoptosis/efectos de los fármacos , Línea Celular Tumoral , Proliferación Celular/efectos de los fármacos , Quimioterapia Combinada , Femenino , Humanos , Masculino , Ratones , FN-kappa B/fisiología , Sarcoma 180/patologíaRESUMEN
The relation between gall stones and chronic pancreatitis is uncertain; there are differing opinions on this issue. Firstly, gall stones are the most common reason for acute pancreatitis, but it cannot cause chronic pancreatitis. Secondly, a connection between gall stones and chronic inflammation of the pancreas might exist. Numerous studies or investigations have shown that changes associated with chronic pancreatitis are common in gallstone patients. Although it seems that gall stones might be a cause of chronic pancreatitis according to these findings, clinical and experimental studies are still needed for confirmation, and further studies are required to determine the mechanisms involved.
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Cálculos Biliares/complicaciones , Pancreatitis Crónica/etiología , Colelitiasis/complicaciones , Colestasis/complicaciones , HumanosRESUMEN
Various integration events from simple to complex have been found in transgenic elite rice varieties, which were co-transformed with bar and cecropin B gene by particle bombardment after system studies of the structure and expression of the transgene. It was displayed that there were one to seven insertion loci and one to ten copies of transgene in different transformants. No absolute correlation was found between the numbers of transgene copies and the gene expression. Different level expression of transgenes with similar insertion mode was also discovered. The expression cassette integrity and transcription frequency of selectable bar gene were more than non-selectable cecropin B gene, whereas gene silence in plants integrated with intact bar gene was found and higher expression level of cecropin B gene with 0.6 kb fragments degraded in terminator was deduced.
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Oryza/genética , Transformación Genética , Transgenes , Northern Blotting , Southern Blotting , Plantas Modificadas GenéticamenteRESUMEN
OBJECTIVE: We hypothesized that tendency toward psychopathology is associated with lower frontotemporal volumes. BACKGROUND: Although there is considerable evidence for structural abnormalities in patients with major psychiatric disorders and increased recognition that neural substrates may underlie individual differences in personality, there have been no studies in healthy people attempting to relate personality to volumetric measures of brain structure. METHOD: We used magnetic resonance imaging with an advanced method for automated segmentation of cranial compartments to gray matter, white matter, and cerebrospinal fluid. We examined the relation between frontal and temporal lobe volumes and Minnesota Multiphasic Personality Inventory measures of tendency toward psychopathology in 59 healthy individuals. RESULTS: As hypothesized, higher scores on the clinical scales were associated with lower average frontal lobe volume. When the sample was divided according to sex, however, these correlations were significant in men (n = 29) but not in women (n = 30). The highest correlation was observed between lower frontal white matter volume in men and high schizophrenia scale score (r[27] = -0.59, p <0.001). CONCLUSIONS: The findings suggest that personality dimensions in healthy people can be linked to neural substrates, which can potentially serve as endophenotypic markers of disposition to psychopathology. The sexually dimorphic effects are consistent with gender-related differences in the clinical manifestations of psychiatric disorders and may suggest sex hormone modulation of the psychopathologic processes.
Asunto(s)
Lóbulo Frontal/anatomía & histología , MMPI , Imagen por Resonancia Magnética , Personalidad/fisiología , Adulto , Biomarcadores , Femenino , Lóbulo Frontal/patología , Predisposición Genética a la Enfermedad , Humanos , Masculino , Personalidad/genética , Psicopatología , Valores de Referencia , Distribución por SexoRESUMEN
In this work, we developed, implemented, and validated an image-processing system for qualitative and quantitative volumetric analysis of brain images. This system allows the visualization and quantitation of global and regional brain volumes. Global volumes were obtained via an automated adaptive Bayesian segmentation technique that labels the brain into white matter, gray matter, and cerebrospinal fluid. Absolute volumetric errors for these compartments ranged between 1 and 3% as indicated by phantom studies. Quantitation of regional brain volumes was performed through normalization and tessellation of segmented brain images into the Talairach space with a 3D elastic warping model. Retest reliability of regional volumes measured in Talairach space indicated errors of < 1.5% for the frontal, parietal, temporal, and occipital brain regions. Additional regional analysis was performed with an automated hybrid method combining a region-of-interest approach and voxel-based analysis, named Regional Analysis of Volumes Examined in Normalized Space (RAVENS). RAVENS analysis for several subcortical structures showed good agreement with operator-defined volumes. This system has sufficient accuracy for longitudinal imaging data and is currently being used in the analysis of neuroimaging data of the Baltimore Longitudinal Study of Aging.