RESUMEN
Matairesinol is one of the lignan compounds found in a variety of plant foodstuffs. We investigated the immunomodulatory effects of (-)-matairesinol in vivo and ex vivo by using mice. Although we found no significant differences in the IgG, IgA and IgM levels in the serum, the IgE level was strongly suppressed by the uptake of (-)-matairesinol in both intact and ovalbumin-immunized mice. The immunoglobulin produced by lymphocytes from the spleen was not activated by the intake of (-)-matairesinol. However, lymphocytes in such gut-associated lymphatic tissues as Peyer's patches and mesenteric lymph nodes were activated by the administration of (-)-matairesinol.
Asunto(s)
Furanos/farmacología , Factores Inmunológicos/farmacología , Lignanos/farmacología , Animales , Femenino , Inmunización , Inmunoglobulina E/biosíntesis , Inmunoglobulina E/sangre , Ganglios Linfáticos/citología , Linfocitos/efectos de los fármacos , Linfocitos/inmunología , Linfocitos/metabolismo , Ratones , Ratones Endogámicos BALB C , Tamaño de los Órganos/efectos de los fármacos , Tamaño de los Órganos/inmunología , Ovalbúmina/inmunología , Ganglios Linfáticos Agregados/citología , Bazo/anatomía & histología , Bazo/efectos de los fármacos , Bazo/inmunologíaRESUMEN
The IgE-suppressive activity of (-)-matairesinol is demonstrated, and the structure-activity relationship of (-)-matairesinol clarified. 3',4-Dihydroxy-3,4'-dimethoxylignano-9,9'-lactone showed higher IgE-suppressive activity than (-)-matairesinol without any cytotoxic activity. Some derivatives bearing a longer and more bulky alkoxy group at the 3 or 4 position showed IgE-accelerative activity.
Asunto(s)
Furanos/química , Furanos/farmacología , Inmunoglobulina E/efectos de los fármacos , Lignanos/química , Lignanos/farmacología , Animales , Línea Celular , Supervivencia Celular/efectos de los fármacos , Furanos/toxicidad , Humanos , Lactonas , Lignanos/toxicidad , Relación Estructura-ActividadRESUMEN
It is well known that heavy oil such as pollutant caused serious influences on the marine ecosystem. We may suffer from various disorders in our body via intake of marine foods polluted with heavy oil. However the influences of heavy oil on our immune system have not yet been clarified. Here we show the effects of heavy oil extracts, water-soluble fraction (WSF), methanol-soluble fraction (MSF) and ethanol-soluble fraction (ESF), on immunoglobulin production of mouse splenocytes. All extracts increased IgA productivity of splenocytes. In oral administration, shrinkage of the immune organs such as spleen or thymus was observed in only WSF-administrated mice at least during 7 days. The amount of IgG production level in splenocytes cultured medium and sera were reduced by each extract administration. A flowcytometry method, to monitor splenocytes of WSF-administrated mice, has been set up using double staining with B and T cell-specific surface antibody. The results from cell population analysis indicated that B cells, including plasma cells producing antibody were reduced. The decrease in IgG level in sera was caused by reduction of plasma cells in spleen. Hence, it is suggested that reduction of Ig production was affected by the chemical compounds contained in WSF possibly such as polycyclic aromatic hydrocarbons (PAHs) through the estrogen receptor expressed in lymphocytes.
Asunto(s)
Estrógenos/toxicidad , Aceites Combustibles/toxicidad , Sistema Inmunológico/efectos de los fármacos , Administración Oral , Animales , Fraccionamiento Químico , Femenino , Inmunoglobulina G/sangre , Antígenos Comunes de Leucocito/metabolismo , Ratones , Tamaño de los Órganos/efectos de los fármacos , Bazo/efectos de los fármacos , Bazo/metabolismo , Timo/efectos de los fármacosRESUMEN
The inhibitory effect of (-)-, (+)-matairesinol and (-)-, (+)-secoisolariciresinol on the discoloration of dark muscle (chiai in Japanese) of two-year-old yellowtail (hamachi in Japanese) was evaluated by measuring the X and a(*) values. (-)-Matairesinol was most effective for retaining the red color of dark muscle in this experiment.
Asunto(s)
Butileno Glicoles/farmacología , Color/normas , Conservación de Alimentos/métodos , Furanos/farmacología , Lignanos/farmacología , Carne , Músculos/efectos de los fármacos , Perciformes , Animales , Antioxidantes/farmacología , Antioxidantes/toxicidad , Peso Corporal/efectos de los fármacos , Butileno Glicoles/toxicidad , Femenino , Furanos/toxicidad , Lignanos/toxicidad , Reacción de Maillard/efectos de los fármacos , Ratones , Ratones Endogámicos BALB C , SolucionesRESUMEN
It is well known that heavy oil pollution results in various negative impacts on the marine environment. Although there is a low possibility of direct exposure to heavy oil, the chemical substances contained in heavy oil may be released into the environment and accumulated by marine organisms which in turn can be taken by humans via the food chain. In this study, we examined the biological risk of heavy oil extract using the common mouse, whose genetic backgrounds and immune system are well known and relatively homologous to humans. Water-soluble fraction (WSF) was extracted from heavy oil with water and the extract orally administrated to female or male mice for 7 days. In the WSF administrated group, cystoma-like formation was observed in the ovary in approximately 80% of female mice. On the other hand, we found that the prostate gland size in male mice was markedly reduced in comparison with male control mice. Continuous administration of WSF for 28 days resulted in continued hypertrophy of the cystoma around the ovary and atrophy in the prostate gland. In addition, it was revealed that chemical substances within WSF have estrogenic activity. A major component of heavy oil, polycyclic aromatic hydrocarbons (PAHs), is known to present estrogenic activity. It is likely that the cystoma-like formation in female mice and atrophy of prostate gland in male resulted of estrogenic substances present in the WSF which might be the PAHs.
Asunto(s)
Mezclas Complejas/toxicidad , Aceites Combustibles/toxicidad , Ovario/efectos de los fármacos , Próstata/efectos de los fármacos , Contaminantes Químicos del Agua/toxicidad , Administración Oral , Animales , Línea Celular Tumoral , Fraccionamiento Químico , Mezclas Complejas/análisis , Estrógenos no Esteroides/análisis , Estrógenos no Esteroides/toxicidad , Femenino , Aceites Combustibles/análisis , Genes Reporteros/efectos de los fármacos , Luciferasas/genética , Luciferasas/metabolismo , Masculino , Ratones , Ovario/patología , Próstata/patología , Organismos Libres de Patógenos Específicos , Agua/químicaRESUMEN
As we have reported, flaxseed lignan, (+)-secoisolariciresinol (SECO), (-)-SECO, and meso-SECO were stereoselectively synthesized and their biological functions were evaluated. In the present study, we focused on the effects of SECOs on the regulation of 3T3-L1 adipocytes, and identified the structure-activity relationships. Optically active SECO and meso-SECO were tested for their effects on lipid metabolism in 3T3-L1 adipocytes. (-)-SECO accelerated adiponectin production of 3T3-L1 adipocytes. On the other hand, (+)- and meso-SECO suppressed the production of adiponectin. In addition, triglyceride (TG) accumulation in 3T3-L1 adipocytes was significantly suppressed by all three SECOs tested here, as was 17beta-estradiol, when the SECOs were added to the medium during induction of 3T3-L1 preadipocytes to adipocytes. Especially, (-)-SECO strongly reduced TG accumulation. It is well-known that SECO has estrogen-like activity. Hence the estrogen-like activity of each SECO compound was assessed. Only (-)-SECO had estrogen-like activity.
Asunto(s)
Adipocitos/efectos de los fármacos , Butileno Glicoles/farmacología , Lignanos/farmacología , Células 3T3-L1 , Adipocitos/citología , Adiponectina/biosíntesis , Animales , Estrógenos , Metabolismo de los Lípidos/efectos de los fármacos , Ratones , Estereoisomerismo , Relación Estructura-Actividad , Triglicéridos/biosíntesisRESUMEN
The antioxidant activity of butane-type lignans was evaluated. Secoisolariciresinol (SECO) and dihydroguaiaretic acid (DGA) showed higher radical scavenging activity than that of 7,7'-dioxodihydroguaiaretic acid (ODGA). SECO and DGA inhibited the oxidation of unsaturated fatty acid. Both enantiomers of DGA were also lipoxygenase inhibitors, but neither enantiomer of SECO inhibited the lipoxygenase activity.