RESUMEN
We studied the effect of tripeptide Leu-Ile-Lys on the course of chronic 16-week oxalate nephrolithiasis in rats modeled by administration of 1% ethylene glycol solution in drinking water for 16 weeks. The tripeptide Leu-Ile-Lys obtained by chemical synthesis (sample purity ≥98%) was administered intragastrically through a probe in a dose of 11.5 mg/kg in 1 ml saline. It was found that during tripeptide Leu-Ile-Lys significantly alleviated the course of experimental pathology, which was confirmed by characteristic biochemical and morphological indicators. We observed a decrease in the concentration of calcium ions by 4.4 times, weakening of oxidative stress in the renal tissue due to a decrease in the total prooxidant activity by 1.2 times, normalization of increased catalase activity, and reduction of superoxide dismutase activity by 2.4 times relative to disease control. Histological signs of nephrolithiasis were recorded in 9% cases (vs. 75% cases in disease control).
Asunto(s)
Nefrolitiasis/tratamiento farmacológico , Péptidos/uso terapéutico , Animales , Calcio/metabolismo , Catalasa/metabolismo , Modelos Animales de Enfermedad , Masculino , Estrés Oxidativo/efectos de los fármacos , Distribución Aleatoria , Ratas , Ratas Wistar , Superóxido Dismutasa/metabolismoRESUMEN
We studied the effect of Leu-Ile-Lys tripeptide on the course of experimental oxalate nephrolithiasis modeled in rats by administration of 1% ethylene glycol solution instead of drinking water for 6 weeks. The Leu-Ile-Lys tripeptide obtained by chemical synthesis (purity ≥98%) was administered through a gastric tube (11.5 mg/kg in 1 ml saline). Administration of Leu-Ile-Lys tripeptide against the background of experimental oxalate nephrolithiasis significantly alleviated the course of experimental pathology, which was confirmed by typical biochemical and morphological changes: decrease in urinary activity of γ-glutamyltransferase (by 2.1 times in comparison with the initial level) and intensity of oxidative stress (the content of TBA-reactive products decreased by 1.3 times in comparison with that in untreated animals) and increase in glutathione peroxidase activity by 1.8 times; no histological signs of nephrolithiasis were found in animals treated with the tripeptide.
Asunto(s)
Antioxidantes/uso terapéutico , Nefrolitiasis/tratamiento farmacológico , Nefrolitiasis/patología , Fragmentos de Péptidos/uso terapéutico , Animales , Antioxidantes/química , Antioxidantes/farmacología , Modelos Animales de Enfermedad , Radicales Libres/metabolismo , Radicales Libres/orina , Pruebas de Función Renal , Masculino , Nefrolitiasis/orina , Fragmentos de Péptidos/farmacología , Ratas , Ratas Wistar , UrinálisisRESUMEN
AIM: to study the pathologic features of chronic oxalate stone disease. MATERIALS AND METHODS: a model of the experimental oxalate stone disease was done in rats that drank the 1% ethylene glycol solution for 16 weeks. The pathologic analysis of the rats kidneys was done. RESULTS: After 16 weeks of oxalate stone disease modeling in all parts of kidneys the large crystals deposits were found, which plugged renal tubules. Moreover, purulent calculous pyelonephritis developed complicated by fibrosis.
Asunto(s)
Cálculos Renales , Cálculos Urinarios , Animales , Oxalato de Calcio , Glicol de Etileno , Riñón , Ratas , Factores de TiempoRESUMEN
Intermittent hypercapnic hypoxia was accompanied by increases in cerebral vascular resistance, decreases in the reactivity of cerebral vessels to hypercapnia, and increases in the collateral reserve and rate of autoregulation of brain blood flow. These changes were an important component of the mechanism increasing the tolerance of the brain to ischemia in response to training with hypercapnic hypoxia.
Asunto(s)
Circulación Cerebrovascular/fisiología , Hipercapnia/fisiopatología , Hipoxia/fisiopatología , Adaptación Fisiológica , Adulto , Velocidad del Flujo Sanguíneo/fisiología , Femenino , Humanos , Arteria Cerebral Media/fisiología , Factores de TiempoRESUMEN
The effectiveness of hypercapnic hypoxic training in the prevention of acute disturbances in cerebral circulation was studied under experimental conditions. Hypercapnic hypoxic training was followed by a significant decrease in the severity of neurological deficit and locomotor and coordination disorders after cerebral ischemic injury.